Long-Term Primary Culture of Human Papillary Thyroid Carcinoma Cells_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

BIAN Xuehai ¹ , ZHAO Jisheng ² , ZHANG Xiuying ³ , ZHANG Chunhai ¹ , FU Yantao ¹ , ZHAO Lina ¹ , SUN Hui ¹ , ZHENG Zelin. ²

1.Department of Thyroid Surgery， ChinaJapan Union Hospital of Jilin University, Changchun 130033， Jilin Province, China;;
2.Department of Gastrointestinal Surgery， ChinaJapan Union Hospital of Jilin University, Changchun 130033， Jilin Province, China;;
3. School of Nursing of Jilin University, Changchun 130033， Jilin Province, China;

Corresponding author：

Keywords：

Papillary thyroid carcinoma; Primary culture; Thyroglobulin; Thyroid peroxidase; Immunohistochemistry

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ObjectiveTo investigate the primary culture method of human papillary thyroid carcinoma (PTC) cells for a long term and establish a monitoring and verification measures. MethodsPTC cells were isolated following routine procedures and cultured in the DMEM supplemented with 10% fetal bovine serum, glutamine, and 20 ng/ml epidermal growth factor (EGF). Thyroglobulin (Tg) and thyroperoxidase (TPO) in nutrient solution and specific antigen Tg expression of PTC cells cultured for different days were observed. ResultsThe PTC cells grew satisfactorily up to 45 days of incubation. Tg content in nutrient solution expressed the training period of a linear singular parabolic, achieved peak value (985.2 μg/L) at about 14 d. TPO had not been detected in nutrient solution. The Tg expressed positively by immunization fluorescent dyeing. ConclusionsPTC cells cultured in the present method can survive to over 45 days. A brief monitoring and evaluation systems of PTC cells has been established. This report prompts that cultured cells within 14 days maybe more suitable to gene research and provide alternative to the basic research of PTC events and features.

Citation： BIAN Xuehai,ZHAO Jisheng,ZHANG Xiuying,ZHANG Chunhai,FU Yantao,ZHAO Lina,SUN Hui,ZHENG Zelin.. Long-Term Primary Culture of Human Papillary Thyroid Carcinoma Cells. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2011, 18(8): 840-843. doi: Copy

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14.	刘坤，罗军，娄繁，等．大鼠骨髓基质细胞的体外培养和鉴定 [J]．中国普外基础与临床杂志， 2009， 16（11）： 875879．.
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16.	黄颖泓，杨立勇．人甲状腺细胞体外培养的研究进展 [J]．医学综述， 2009， 15（10）： 14551458．.
17.	Visser TJ. The elemental importance of sufficient iodine intake: a trace is not enough [J]. Endocrinology, 2006, 147(5): 20952097.
18.	Mazzaferri EL, Robbins RJ, Spencer CA, et al. A consensus report of the role of serum thyroglobulin as a monitoring method for lowrisk patients with papillary thyroid carcinoma [J]. J Clin Endocrinol Metab, 2003, 88(4): 14331441.
19.	Miyauchi A, Kudo T, Miya A, et al. Prognostic impact of serum thyroglobulin doublingtime under thyrotropin suppression in patients with papillary thyroid carcinoma who underwent total thyroidectomy [J]. Thyroid, 2011, 21(7): 707716.
20.	陈佳瑞，王家东. 桥本甲状腺炎合并甲状腺乳头状癌外科诊治(附51例临床分析) [J]. 临床耳鼻咽喉头颈外科杂志， 2010, 24(6): 257260.
21.	Hartl DM, Al GA, Chami L, et al. High rate of multifocality and occult lymph node metastases in papillary thyroid carcinoma arising in thyroglossal duct cysts [J]. Ann Surg Oncol, 2009, 16(9): 25952601.
22.	莫祥兰，黄振录，周祥祯，等．高分子量角蛋白和TPO在甲状腺乳头状癌诊断中的价值 [J]．中国实验诊断学， 2006， 10（6）： 585587．.
23.	Müller K, Krohn K, Eszlinger M, et al. Effect of iodine on early stage thyroid autonomy [J]. Genomics, 2011, 97(2): 94100.
24.	Faggiano A, Severino R, Ramundo V, et al. Thyroid function in fabry disease before and after enzyme replacement therapy [J]. Minerva Endocrinol, 2011, 36(1): 15.
25.	Ott J, Promberger R, Kober F, et al. Hashimoto’s thyroiditis affects symptom load and quality of life unrelated to hypothyroidism: a prospective casecontrol study in women undergoing thyroidectomy for benign goiter [J]. Thyroid, 2011, 21(2): 161167.

1. Woodruff SL, Arowolo OA, Akute OO, et al. Global variation in the pattern of differentiated thyroid cancer [J]. Am J Surg, 2010, 200(4): 462466.
2. Pedersen IB, Laurberg P, Knudsen N, et al. An increased incidence of overt hypothyroidism after iodine fortification of salt in denmark:a prospective population study [J]. J Clin Endocrinol Metab, 2007, 92(8): 31223127.
3. 边学海，张广，张纯海，等．甲状腺乳头状癌组织中Shh和Gli1的表达及其临床意义研究 [J]．国际外科学杂志， 2011， 38（2）： 7881．.
4. Delellis RA. Pathology and genetics of thyroid carcinoma [J]. J Surg Oncol, 2006, 94(8): 662669.
5. Troviseo V, Soares P, Preto A, et al. Molecular genetics of papillary thyroid carcinoma: great expectations [J]. Arq Bras Endocrinol Metabol, 2007, 51(5): 643653.
6. 任立军，杨延芳，张成雷．甲状腺乳头状癌淋巴结转移规律的临床研究 [J]．中国普外基础与临床杂志， 2011， 18（4）： 419422．.
7. 于金明，徐忠法．肿瘤临床通鉴 [M]．济南：山东科学技术出版社， 2004： 369375．.
8. Mazzaferri EL, Jhiang SM. Longterm impact of initial surgical and medical therapy on papillary and follicular thyroid cancer [J]. Am J Med, 1994, 97(5): 418428.
9. 吴立刚，刘奇伦，胡志强． MMP2和VEGFC在甲状腺乳头状癌中的表达及临床意义 [J]．中国普外基础与临床杂志， 2009， 16（11）： 906910．.
10. 袁振芳，高研，王薇，等． γ干扰素对培养的人甲状腺细胞HLADR抗原基因表达的影响 [J]．中华内科杂志， 2002， 41（4）： 262263．.
11. 刘东方，邓华聪，许改平，等．人甲状腺原代细胞的长期培养 [J]．中华内分泌代谢杂志， 2005， 21（2）： 181183．.
12. Jeker LT, Mehrdad H, Lynne BC, et al. Mouse thyroid primary culture [J]. Biochem Biophys Res Commun, 1999, 257(2): 511515.
13. Antonelli A, Ferrari SM, Fallahi P, et al. Primary cell cultures from anaplastic thyroid cancer obtained by fineneedle aspiration used for chemosensitivity tests [J]. Clin Endocrinol (Oxf), 2008, 69(1): 148152.
14. 刘坤，罗军，娄繁，等．大鼠骨髓基质细胞的体外培养和鉴定 [J]．中国普外基础与临床杂志， 2009， 16（11）： 875879．.
15. Di FM, Coleoni AH, Pellizas CG, et al. Triiodothyronine induces proliferation in cultured bovine thyroid cells: evidence for the involvement of epidermal growth factorassociated tyrosine kinase activity [J]. J Endocrinol, 2000, 166(1): 173182.
16. 黄颖泓，杨立勇．人甲状腺细胞体外培养的研究进展 [J]．医学综述， 2009， 15（10）： 14551458．.
17. Visser TJ. The elemental importance of sufficient iodine intake: a trace is not enough [J]. Endocrinology, 2006, 147(5): 20952097.
18. Mazzaferri EL, Robbins RJ, Spencer CA, et al. A consensus report of the role of serum thyroglobulin as a monitoring method for lowrisk patients with papillary thyroid carcinoma [J]. J Clin Endocrinol Metab, 2003, 88(4): 14331441.
19. Miyauchi A, Kudo T, Miya A, et al. Prognostic impact of serum thyroglobulin doublingtime under thyrotropin suppression in patients with papillary thyroid carcinoma who underwent total thyroidectomy [J]. Thyroid, 2011, 21(7): 707716.
20. 陈佳瑞，王家东. 桥本甲状腺炎合并甲状腺乳头状癌外科诊治(附51例临床分析) [J]. 临床耳鼻咽喉头颈外科杂志， 2010, 24(6): 257260.
21. Hartl DM, Al GA, Chami L, et al. High rate of multifocality and occult lymph node metastases in papillary thyroid carcinoma arising in thyroglossal duct cysts [J]. Ann Surg Oncol, 2009, 16(9): 25952601.
22. 莫祥兰，黄振录，周祥祯，等．高分子量角蛋白和TPO在甲状腺乳头状癌诊断中的价值 [J]．中国实验诊断学， 2006， 10（6）： 585587．.
23. Müller K, Krohn K, Eszlinger M, et al. Effect of iodine on early stage thyroid autonomy [J]. Genomics, 2011, 97(2): 94100.
24. Faggiano A, Severino R, Ramundo V, et al. Thyroid function in fabry disease before and after enzyme replacement therapy [J]. Minerva Endocrinol, 2011, 36(1): 15.
25. Ott J, Promberger R, Kober F, et al. Hashimoto’s thyroiditis affects symptom load and quality of life unrelated to hypothyroidism: a prospective casecontrol study in women undergoing thyroidectomy for benign goiter [J]. Thyroid, 2011, 21(2): 161167.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Long-Term Primary Culture of Human Papillary Thyroid Carcinoma Cells

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