Experimental Study about Inhibitory Effect of Iodine-125 on Moderately Differentiated Adenocarcinoma of Colon_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

LI Yingxu , LUO Kaiyuan , CHEN Yi , GUO Jin , TANG Jing.

Department of General Surgery, The Fourth Affiliated Hospital, Kunming Medical College, Kunming 650021, ChinaCorresponding Author: LUO Kai-yuan, E-mail: ynluoky@yahoo.com.cn;

Corresponding author：

Keywords：

Iodine-125 particle; Moderately differentiated adenocarcinoma of colon; Proliferation; Apoptosis

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Objective 　To approach the inhibitory effect of Iodine-125 (125I) on moderately differentiated adenocarcinoma of colon by establishing the nude mice model bearing subcutaneous tumor of SW480 cell.
Methods　 The moderately differentiated adenocarcinoma of colon cells (SW480) were implanted subcutaneously to the nude mice. The bearing tumor nude mice were randomly divided into study group (n=24) and control group (n=24) by using method of random sampling. One blank particle was implanted into the mouse of the control group, a 1.48×107 Bq dosage 　125I particle was implanted into the mouse of the study group, then the growth of tumor was observed after implantation. Six bearing tumor nude mice were sacrificed and the tumors were obtained on day 7, 14, 21, and 28 after implantation, respectively. The expression of proliferating cell nuclear antigen (PCNA) was detected by immunohistochemistry SP method. The cell apoptosis was determined by TUNEL method.
Results　 As the accumulation of radiation time, the volume of tumor in the study group was smaller than that in the control group on day 10 after implantation (P lt;0.05). The PCNA labeling index in the study group was lower than that in the control group on day 14 after implantation (P lt;0.05). The apoptotic index in the study group was higher than that in the control group on day 21 after implantation (P lt;0.05).
Conclusion 　Persistent low dose 　125I radiation could down-regulate the expression of PCNA, and induce the apoptosis of moderately differentiated adenocarcinoma of colon cell, which might be a mechanism of inhibiting the proliferation of moderately differentiated adenocarcinoma of colon.

Citation： LI Yingxu,LUO Kaiyuan,CHEN Yi,GUO Jin,TANG Jing.. Experimental Study about Inhibitory Effect of Iodine-125 on Moderately Differentiated Adenocarcinoma of Colon. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2010, 17(10): 1062-1066. doi: Copy

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13.	Lee CH, Lee MK, Kang CD, et al. Differential expression of hypoxia inducible factor-1 alpha and tumor cell proliferation between squamous cell carcinomas and adenocarcinomas 　among operable non-small cell lung carcinomas [J]. J Korean Med Sci, 2003; 18(2): 196-203．.
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1. 　Kemp Z, Thirlwell C, Sieber O, et al. An update on the genetics of colorectal cancer [J]. Hum Mol Genet, 2004; 13(2)： R177-R185．.
2. 　郁宝铭. 结直肠癌外科治疗的现状和展望 [J]. 中国普外基础与临床杂志, 2008; 15(9)： 631-636.
3. 　Chedid AD, Villwock Mde M, Chedid MF, et al. Prognostic factors following liver resection for hepatic metastases from colorectal cancer [J]. Arq Gastroenterol, 2003; 40(3): 159-165.
4. 　王宁, 黄志勇． PCNA和CEA在胃粘膜上皮异型增生和胃癌中的表达 [J]. 诊断病理学杂志, 1994; 1(2): 94-96．.
5. 　戴林, 张学庸．增殖细胞核抗原与胃癌 [J]．国外医学消化系疾病分册, 1996; 16(1): 3-5．.
6. 　Nasif WA, El-Emshaty HM, Lotfy M, et al. Apoptosis dysregulation in human gastric carcinomas: relationship to anti- and pro-apoptotic protein expression [J]. Asian Pac J Cancer Prev, 2007; 8(1): 45-50．.
7. 　柴树德, 郑广钧．粒子植入治疗胸部肿瘤　[M]．天津: 天津科技出版社, 2007: 260-265．.
8. 　付改发, 雷光焰, 赵竹莲, 等. 　125I新型粒子永久植入治疗恶性肿瘤的应用与观察 [J]．现代肿瘤医学, 2004; 12(2): 108-109．.
9. 　王俊杰, 修典荣, 冉维强．粒子组织间治疗肿瘤　[M]．第2版．北京：北京大学医学出版社， 2004： 49-56．.
10. 　Hartwell LH, Kastan MB. Cell cycle control and cancer [J]. Science, 1994; 266(5192): 1821-1828．.
11. 　Nasmyth K. Viewpoint: putting the cell cycle in order [J]. Science, 1996; 274(5293): 1643-1645．.
12. 　Rizzo MG, Ottavio L, Travali S, et al. The promoter of the human proliferating cell nuclear antigen (PCNA) gene is bidirectional [J]. Exp Cell Res, 1990; 188(2): 286-293.
13. 　Lee CH, Lee MK, Kang CD, et al. Differential expression of hypoxia inducible factor-1 alpha and tumor cell proliferation between squamous cell carcinomas and adenocarcinomas 　among operable non-small cell lung carcinomas [J]. J Korean Med Sci, 2003; 18(2): 196-203．.
14. 　Israels ED, Israels LG. The cell cycle [J]. Stem Cells, 2001; 19(1): 88-91．.
15. 　魏道严, 金敖兴, 黄帼, 等．小剂量辐射对小鼠肿瘤的抑制作用 [J]. 中华放射肿瘤学杂志, 1995; 4(4)： 261.
16. 　Kerr JF, Wyllie AH, Currie AR. Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics [J]. Br J Cancer, 1972; 26(4): 239-257．.
17. 　Zou JP, Shimizu J, Ikegame K, et al. Tumor-immunotherapy with the use of tumor-antigen-pulsed antigen-presenting cells [J]. Cancer Immunol Immunother, 1992; 35(1): 1-6.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Experimental Study about Inhibitory Effect of Iodine-125 on Moderately Differentiated Adenocarcinoma of Colon

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