Expression of Fascin-1 Protein in Colorectal Adenocarcinoma and Relation with Its Clinicopathologic Characteristics_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

LI Hongwei , WANG Tieshan , HUANG Yong , LI Yanhong , HU Haixia.

Department of Pathology, The 251st Hospital of PLA, Zhangjiakou 075000, Hebei Province, ChinaCorresponding Author: LI Hong-wei, E-mail: lhwei194@163.com;

Corresponding author：

Keywords：

Colorectal adenocarcinoma; Invasion; Metastasis; Fascin-1 protein; Immunohistochemistry

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　Objective 　To investigate the expression of Fascin-1 protein in colorectal adenocarcinoma, and the relationship with its clinicopathologic features.
　Methods 　The expressions of Fascin-1 protein in colorectal adenocarcinoma tissues of 60 cases, colorectal adenoma tissues of 30 cases and normal mucosa tissues (4 cm distance to neoplasm) of 30 cases were detected by Microwave-EliVisionTM immunohistochemistry method, and the relationship between the expression of Fascin-1 protein in colorectal adenocarcinoma tissues and its clinicopathologic characteristics was analyzed.
　Results 　The expression of Fascin-1 protein was located in cytoplasm. The positive expression rates of Facsin-1 protein were 3.3% (1/30), 30.0% (9/30) and 53.3% (32/60) in normal mucosa tissues, colorectal adenoma tissues and colorectal adenocarcinoma tissues, respectively. The expression of Fascin-1 was gradually increased in these three tissues, and there was statistical difference among the three tissues (P lt;0.05). The expessions of Fascin-1 protein in patients with serous membrane invasion, lymph node metastasis and TNM Ⅲ+Ⅳ were higher than those of non-serous membrane invasion, non-lymph node metastasis and TNM Ⅰ+Ⅱ (P lt;0.05), but there was no significant difference among different differentiation degrees (P gt;0.05).
　Conclusion 　The high expression of Fascin-1 protein is correlated to high invasion ability and lymph node metastasis, which can play as a sensitive index in predicting the invasion and metastasis of colorectal adenocarcinoma.

Citation： LI Hongwei,WANG Tieshan,HUANG Yong,LI Yanhong,HU Haixia.. Expression of Fascin-1 Protein in Colorectal Adenocarcinoma and Relation with Its Clinicopathologic Characteristics. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2010, 17(4): 362-364. doi: Copy

1.	熊正文. 免疫组织化学技术中抗原修复的研究进展 [J].中华病理学杂志, 1997; 26(2): 124-126.
2.	许良中, 杨文涛. 免疫组织化学反应结果的判断标准 [J]. 中国癌症杂志, 1996; 6(4): 229-231.
3.	Bryan J, Kane RE. Separation and interaction of themajor components of sea urchin actin gel [J]. J Mol Biol, 1978; 125(2): 207-224.
4.	Guo W, Giancotti FG. Integrin signalling during tumour progresssion [J]. Nat Rev Mol Cell Biol, 2004; 5(10): 816-826.
5.	Ono S, Yamakita Y, Yamashim S, et al. Dentification of an actin binding region and a protein kinase C phoep horylation site on human fascin [J]. J Biol Chem, 1997; 272(4): 2527-2533.
6.	Adams JC, Clelland JD, Collett GD, et al. Cell-matrix adhesions diff-erenttially regulate fascin phosphorylation [J]. Mol Biol Cell, 1999; 10(12): 4177-4190.
7.	荣举, 许丽艳, 蔡唯佳, 等. Fasin-1基因在永生化食管上皮细胞癌变中的表达 [J]. 癌症, 2004; 23(3): 243-248.
8.	Hashimoto Y, Ito T, Inoue H, et al. Prognostic significance of fascin overexpression in human esophageal squamouscell carcinoma [J]. Clin Cancer Res, 2005; 11(7): 2597-2605.
9.	Yoder BJ, Tso E, Skacel M, et al. The expression of fascin, an actin-bunding motility protein, correlates with homone receptor-negative breast cancer and amore aggressi clinical course [J]. Clin Cancer Res, 2005; 11(1): 186-192.
10.	Xie JJ, Xu LY, Zhang HH, et al. Role of fascin in the proliferation and invasiveness of esophagea carcinoma cells [J]. Biochem Biophys Res Commun, 2005; 337(1): 355-362.
11.	Pelosi G, Pastorino U, Pasini F, et al. Independent prognostic value of fascin immunoreactivity in stage Ⅰ nonsmall cell lung cancer [J]. Br J Cancer, 2003; 24(88): 537-547.
12.	Hashimoto Y, Shimada Y, Kawamura J, et al. The prognostic relevance of Fascin expression in human gastric carcinoma [J]. Oncology, 2004; 67(3-4): 262-270.
13.	杨强, 卢朝辉, 罗玉凤, 等. fascin 蛋白在结、直肠腺瘤和癌中的表达及意义 [J]. 诊断病理学杂志, 2006； 13(5): 359-361.
14.	高翔, 吴德华. FSCN1蛋白在人上皮性肿瘤组织中的表达及其临床意义 [J]. 南方医科大学学报, 2008; 28(6): 953-955.
15.	Jawhari AU, Buda A, Jenkins M, et al. Fascin, an actin-bundling protein, modulates colonic epithelial cell invasiveness and differentiation in vitro [J]. Am J Pathol, 2003; 162(1): 69-80.
16.	Iguchi T, Aishima S, Umeda K, et al. Fascin expression in progression and prognosis of hepatocellular carcinoma [J]. J Surg Oncol, 2009; 100(7): 575-579.
17.	Kefeli M, Yildiz L, Kaya FC, et al. Fascin expression in uterine smooth muscle tumors [J]. Int J Gynecol Pathol, 2009; 28(4): 328-333.
18.	Qualtrough D, Singh K, Banu N. The actin-bundling protein fascin is overexpressed in colorectal adenomas and promotes motility in adenoma cells in vitro [J]. Br J Cancer, 2009; 101(7): 1124-1129.
19.	Tao YS, Edwards RA, Tubb B, et al. beta-Catenin associates with the actin-bunding protein fascin in a noncadherin complex [J]. J Cell Biol, 1996; 134(5): 1271-1281.
20.	Vignjevic D, Schoumacher M, Gavert N, et al. Fascin, a novel target of beta-catenin-TCF signaling, is expressed at the invasive front of human colon cancer [J]. Cancer Res, 2007; 67(14): 6844-6853.
21.	Oving IM, Clevers HC. Molecular causes of colon cancer [J]. Eur J Clin Invest, 2002; 32(6): 448-457.

1. 熊正文. 免疫组织化学技术中抗原修复的研究进展 [J].中华病理学杂志, 1997; 26(2): 124-126.
2. 　许良中, 杨文涛. 免疫组织化学反应结果的判断标准 [J]. 中国癌症杂志, 1996; 6(4): 229-231.
3. 　Bryan J, Kane RE. Separation and interaction of themajor components of sea urchin actin gel [J]. J Mol Biol, 1978; 125(2): 207-224.
4. 　Guo W, Giancotti FG. Integrin signalling during tumour progresssion [J]. Nat Rev Mol Cell Biol, 2004; 5(10): 816-826.
5. 　Ono S, Yamakita Y, Yamashim S, et al. Dentification of an actin binding region and a protein kinase C phoep horylation site on human fascin [J]. J Biol Chem, 1997; 272(4): 2527-2533.
6. 　Adams JC, Clelland JD, Collett GD, et al. Cell-matrix adhesions diff-erenttially regulate fascin phosphorylation [J]. Mol Biol Cell, 1999; 10(12): 4177-4190.
7. 　荣举, 许丽艳, 蔡唯佳, 等. Fasin-1基因在永生化食管上皮细胞癌变中的表达 [J]. 癌症, 2004; 23(3): 243-248.
8. 　Hashimoto Y, Ito T, Inoue H, et al. Prognostic significance of fascin overexpression in human esophageal squamouscell carcinoma [J]. Clin Cancer Res, 2005; 11(7): 2597-2605.
9. 　Yoder BJ, Tso E, Skacel M, et al. The expression of fascin, an actin-bunding motility protein, correlates with homone receptor-negative breast cancer and amore aggressi clinical course [J]. Clin Cancer Res, 2005; 11(1): 186-192.
10. 　Xie JJ, Xu LY, Zhang HH, et al. Role of fascin in the proliferation and invasiveness of esophagea carcinoma cells [J]. Biochem Biophys Res Commun, 2005; 337(1): 355-362.
11. 　Pelosi G, Pastorino U, Pasini F, et al. Independent prognostic value of fascin immunoreactivity in stage Ⅰ nonsmall cell lung cancer [J]. Br J Cancer, 2003; 24(88): 537-547.
12. 　Hashimoto Y, Shimada Y, Kawamura J, et al. The prognostic relevance of Fascin expression in human gastric carcinoma [J]. Oncology, 2004; 67(3-4): 262-270.
13. 　杨强, 卢朝辉, 罗玉凤, 等. fascin 蛋白在结、直肠腺瘤和癌中的表达及意义 [J]. 诊断病理学杂志, 2006； 13(5): 359-361.
14. 　高翔, 吴德华. FSCN1蛋白在人上皮性肿瘤组织中的表达及其临床意义 [J]. 南方医科大学学报, 2008; 28(6): 953-955.
15. 　Jawhari AU, Buda A, Jenkins M, et al. Fascin, an actin-bundling protein, modulates colonic epithelial cell invasiveness and differentiation in vitro [J]. Am J Pathol, 2003; 162(1): 69-80.
16. 　Iguchi T, Aishima S, Umeda K, et al. Fascin expression in progression and prognosis of hepatocellular carcinoma [J]. J Surg Oncol, 2009; 100(7): 575-579.
17. 　Kefeli M, Yildiz L, Kaya FC, et al. Fascin expression in uterine smooth muscle tumors [J]. Int J Gynecol Pathol, 2009; 28(4): 328-333.
18. 　Qualtrough D, Singh K, Banu N. The actin-bundling protein fascin is overexpressed in colorectal adenomas and promotes motility in adenoma cells in vitro [J]. Br J Cancer, 2009; 101(7): 1124-1129.
19. 　Tao YS, Edwards RA, Tubb B, et al. beta-Catenin associates with the actin-bunding protein fascin in a noncadherin complex [J]. J Cell Biol, 1996; 134(5): 1271-1281.
20. 　Vignjevic D, Schoumacher M, Gavert N, et al. Fascin, a novel target of beta-catenin-TCF signaling, is expressed at the invasive front of human colon cancer [J]. Cancer Res, 2007; 67(14): 6844-6853.
21. 　Oving IM, Clevers HC. Molecular causes of colon cancer [J]. Eur J Clin Invest, 2002; 32(6): 448-457.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Expression of Fascin-1 Protein in Colorectal Adenocarcinoma and Relation with Its Clinicopathologic Characteristics

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