Experimental Study of Serum Proteomics Patterns Detected by SELDI-TOF MS in Early Diagnosis of Hepatic Fibrosis_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

DONG Yongjie ¹ , PENG Yanhui ² , ZHAO Xiaoyun ³

1.Department of General Surgery， The Central Hospital of Handan， Handan 056001， Hebei Province, China;;
2.Department of General Surgery, The People’s Hospital of Hebei Province, Shijiazhuang 050051, China;;
3.Laboratory of Geriatric Medicine of Hebei Province, Shijiazhuang 050051, China;

Corresponding author：

Keywords：

Hepatic fibrosis; Distinct protein; Protein finger printing technology; Proteomics; Protein chip

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Objective To find a new specific marker that can be used to early diagnose hepatic fibrosis by detecting the change of serum protein in patients with hepatic fibrosis.
Methods This research adopted 50 SD rats (25 males and 25 females), and from which 6 rats were selected randomly (3 males and 3 females) as control group, last 44 rates were divided into four groups according to four pathological stages as hepatic fibrosis model group (experimental group). Distinct proteins in serum were detected by surface-enhanced laser desorption/ionization-time of flight-mass spectra (SELDI-TOF-MS). Radioimmunoassay was used to measure four parameters of hepatic fibrosis which were hyaluronidase (HA), precollagen Ⅲ (PCⅢ), laminin (LN) and collagen Ⅳ (Ⅳ-C).
Results Distinct proteins in serum were detected in 8 cases of stage Ⅰ of hapatic fibrosis, 5 cases of stage Ⅱ, 5 cases of stage Ⅲ, 6 cases of stage Ⅳ, and 5 cases of control by SELDI-TOF-MS. Three protein peaks were found (M/Z: 4 203, 4 658, and 7 400). The peaks of M/Z 4 658 and 4 700 proteins were obviously increased in the stage Ⅰ of hepatic fibrosis （P lt;0.05）, while the changes of hepatic fibrosis four parameters appeared in stage Ⅳ of hepatic fibrosis.
Conclusion This method shows great potential for early diagnosing of hepatic fibrosis and finding better biomarkers to hepatic fibrosis.

Citation： DONG Yongjie ,PENG Yanhui,ZHAO Xiaoyun. Experimental Study of Serum Proteomics Patterns Detected by SELDI-TOF MS in Early Diagnosis of Hepatic Fibrosis. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2010, 17(5): 459-462. doi: Copy

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10.	Fields S. Proteomics. Proteomics in genomeland [J]. Scinece, 2001; 291(5507): 1221-1224.
11.	Poon TC, Yip TT, Chan AT, et al. Comprehensive proteomic profiling identifies serum proteomic signatures for detection of hepatocellular carcinoma and its subtypes [J]. Clin Chem,　 2003； 49(5): 752-760.
12.	Zhukov TA， Johnson RA, Cantor AB, et al. Discovery of distinct protein profiles specificfor lung tumor and pre-malignant lung lesions by SELDI mass spectrometry [J]. Lung Cancer, 2003； 40（3）: 267-279.
13.	Cazares LH, Adam BL, Ward MD, et al. Normal, benign, preneoplastic, and malignant prostate cells have distinct protein expression profiles resolved by surface enhanced laser desorption/ionization mass spectrometry [J]. Clin Cancer Res, 2002； 8(8): 2541-2552.

1. 韩经寰. 肝纤维化的研究进展 [J]. 天津医药, 1980； 3（3）: 182-188.
2. Tsukmoto H， Gaal K, French SW. Insights into the pathogenesis of alcoholic liver necrosis and fibrosis: status report [J]. Hepatology, 1990； 12 (3 Pt 1) : 599-608.
3. Petricoin EF, Ardekani AM, Hitt BA, et al. Use of proteomic patterns in serum to identify ovarian cancer [J]. Lancet, 2002； 359（9306）: 572-577.
4. Petricoin EF 3rd, Omstein DK, Paweletz CP, et al. Serum proteomic patterns for detection of prostate cancer [J]. J Natl Cancer Inst, 2002； 94（20）: 1576-1578.
5. Adam BL, Qu Y, Davis JW, et al. Serum protein fingerprinting coupled with a pattern-matching algorithm distinguishes prostate cancer from benign prostate hyperplasia and healthy men. [J]. Cancer Res, 2002； 62（13）: 3609-3614.
6. 许建明, 徐叔云, 张运芳，等. 四氯化碳诱导小鼠肝纤维化模型的建立 [J]. 中国药理学通报, 2000；16(3): 339-341.
7. 苗明三. 实验动物和动物实验技术 [M]. 北京: 中国中医药出版社, 1996： 225-226.
8. 中华医学会传染病与寄生虫病学分会、肝病学分会. 病毒性肝炎防治方案 [J]. 中华内科杂志, 2001； 40(1): 62-68.
9. 柴明胜. 肝病诊断新标志-血清Ⅳ型胶原 [J]. 上海医学检验杂志， 1995; 10(3): 182-183.
10. Fields S. Proteomics. Proteomics in genomeland [J]. Scinece, 2001; 291(5507): 1221-1224.
11. Poon TC, Yip TT, Chan AT, et al. Comprehensive proteomic profiling identifies serum proteomic signatures for detection of hepatocellular carcinoma and its subtypes [J]. Clin Chem,　 2003； 49(5): 752-760.
12. Zhukov TA， Johnson RA, Cantor AB, et al. Discovery of distinct protein profiles specificfor lung tumor and pre-malignant lung lesions by SELDI mass spectrometry [J]. Lung Cancer, 2003； 40（3）: 267-279.
13. Cazares LH, Adam BL, Ward MD, et al. Normal, benign, preneoplastic, and malignant prostate cells have distinct protein expression profiles resolved by surface enhanced laser desorption/ionization mass spectrometry [J]. Clin Cancer Res, 2002； 8(8): 2541-2552.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Experimental Study of Serum Proteomics Patterns Detected by SELDI-TOF MS in Early Diagnosis of Hepatic Fibrosis

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