Influence of Different Pressures and Time of CO2 Pneumoperitoneum on Adhesive and Invasive Ability of Gastric Cancer Cells_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

LIU Hua ¹ , ZHOU Min ² , ZHAO Wenyi ¹ , CAO Hui ¹

1.Department of General Surgery, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200127, China;;
2.Department of Surgery, The Second Hospital Affiliated to Zhejiang University School of Medicine, Zhejiang 310003, China;

Corresponding author：

Keywords：

Gastric cancer cells; CO2 pneumoperitoneum; Adhesive molecule; Invasive molecule; Neoplasm metastasis

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Objective To investigate the influence of different pressures and duration of CO2 pneumoperitoneum on the adhesive and invasive ability of gastric cancer cells based on the expressions of adhesive and invasive molecules. Methods With an artificial CO2 pneumoperitoneum model in vitro, human gastric cancer cell lines including MKN-45, SGC-7901, and MKN-28 were exposed to CO2 in different environments: 0 mm Hg （1 mm Hg=0.133 kPa）, 9 mm Hg （2 h, 4 h）, and 15 mm Hg （2 h, 4 h）. The expressions of mRNA of E-cadherin, intercellular adhesion molecule-1 (ICAM-1), matrix metalloproteinase-2 (MMP-2), and vascular endothelial growth factor-A (VEGF-A) in the different environments were measured by RT-PCR. The expressions of protein of E-cadherin and ICAM-1 in the environments of 0 mm Hg and 15 mm Hg （4 h） were measured by FCM. Results With the increase of duration or pressure, RT-PCR showed that there was a downward trend in the expression of E-cadherin mRNA as well as there were upward trends in the expressions of ICAM-1, MMP-2, and VEGF-A mRNA; FCM showed that there was a downward trend in the expression of E-cadherin protein while the expression of ICAM-1 protein showed the opposite change. But there were no obvious differences under different environment (P＞0.05). Conclusions Under low pressure (≤15 mm Hg) and short time (≤4 h) of CO2 pneumoperitoneum, the adhesive and invasive ability of gastric cancer cells could not be affected, which means that under this environment, CO2 pneumoperitoneum will not increase the possibility of neoplasm metastasis.

Citation： LIU Hua,ZHOU Min,ZHAO Wenyi,CAO Hui. Influence of Different Pressures and Time of CO2 Pneumoperitoneum on Adhesive and Invasive Ability of Gastric Cancer Cells. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2010, 17(6): 557-561. doi: Copy

1.	Metzelder M, Kuebler JF, Shimotakahara A, et al. CO2 pneumoperitoneum increases survival in mice with polymicrobial peritonitis [J]. Eur J Pediatr Surg， 2008; 18(3): 171-175.
2.	余佩武，郝迎学. CO2气腹对胃肠道肿瘤侵袭转移影响的研究现状与进展 [J]. 中国普外基础与临床杂志, 2009; 16(10): 777-780.
3.	Memon MA, Khan S, Yunus RM, et al. Meta-analysis of laparoscopic and open distal gastrectomy for gastric carcinoma [J]. Surg Endosc, 2008; 22(8): 1781-1789.
4.	Topal B, Leys E, Ectors N, et al. Determinants of complications and adequacy of surgical resection in laparoscopic versus open total gastrectomy for adenocarcinoma [J]. Surg Endosc, 2008; 22(4): 980-984.
5.	Person B, Cera SM. Prolonged postlaparoscopy carbon dioxide pneumoperitoneum [J]. Surg Laparosc Endosc Percutan Tech, 2008; 18(1): 114-117.
6.	Fuganti PE, Rodrigues Júnior AJ, Rodrigues CJ, et al. A comparison of the effects of pneumoperitoneum and laparotomy on natural killer cell mediated cytotoxicity and Walker tumor growth in Wistar rats [J]. Surg Endosc, 2006; 20(12): 1858-1861.
7.	Kosugi C, Ono M, Saito N, et al. Port site recurrence diagnosed by positron emission tomography after laparoscopic surgery for colon cancer [J]. Hepatogastroenterology, 2005; 52(65): 1440-1443.
8.	Hirabayashi Y, Yamaguchi K, Shiraishi N, et al. Port-site metastasis after CO2 pneumoperitoneum: role of adhesion molecules and prevention with antiadhesion molecules [J]. Surg Endosc, 2004; 18(7): 1113-1117.
9.	田永刚，许军，代文杰，等. 人工气腹对结肠癌细胞增殖影响的实验研究 [J]. 中华普通外科杂志, 2007; 22(8): 623-626.
10.	马君俊，冯波，张轶，等. CO2气腹对结肠癌细胞侵袭能力影响的体外研究 [J]. 中国普外基础与临床杂志, 2007; 14(5): 512-515.
11.	Hao YX, Zhong H, Zhang C, et al. Effects of simulated carbon dioxide and helium peumoperitoneum on proliferation and apoptosis of gastric cancer cells [J]. World J Gastroenterol, 2008; 14(14): 2241-2245.
12.	Tan BJ, Dy JS, Chiu PY, et al. Effects of pneumoperitoneal gases and pressures on transitional cell carcinoma adhesion, growth, apoptosis and necrosis: an in vitro study [J]. J Urol, 2005; 174(4 Pt 1): 1463-1467.
13.	Peng Y, Zheng M, Feng B, et al. Hyperthermic CO2 pneumoperitoneum induces apoptosis in human colon cancer cells through Bax-associated mitochondrial pathway [J]. Oncol Rep, 2008; 19(1): 73-79.
14.	王广义，孟宪瑛，顾建华，等. CO2气腹对肿瘤细胞种植与生长的影响 [J]. 中国普外基础与临床杂志, 2004; 11(2): 133-135.
15.	Ordemann J, Hflich C, Braumann C, et al. Impact of pneumoperitoneum on expression of E-cadherin, CD44v6 and CD54 (ICAM-1) on HT-29 colon-carcinoma cells [J]. Zentralbl Chir, 2005; 130(5): 405-409.
16.	Yavuz Y, Rnning K, Lyng O, et al. Effect of carbon dioxide pneumoperitoneum on tissue blood flow in the peritoneum, rectus abdominis, and diaphragm muscles [J]. Surg Endosc, 2003; 17(10): 1632-1635.
17.	Schmidt AI, Reismann M, Kübler JF, et al. Exposure to carbon dioxide and helium reduces in vitro proliferation of pediatric tumor cells [J]. Pediatr Surg Int, 2006; 22(1): 72-77.
18.	Ishida H, Hashimoto D, Nakada H, et al. Increased insufflation pressure enhances the development of liver metastasis in a mouse laparoscopy model: possible mechanisms [J]. Surg Endosc, 2002; 16(2): 331-335.
19.	张浩波，王灏，郑民华. 持续性CO2气腹影响腹腔镜手术切口肿瘤细胞种植的实验研究 [J]. 中国普外基础与临床杂志, 2002; 9(1): 16-18.
20.	石彦，郝迎学，雷晓，等. 模拟CO2气腹对人胃癌MKN-45细胞增殖和细胞周期的影响 [J]. 第三军医大学学报, 2007; 29(23): 2274-2276.
21.	Tosun M, Samli H, Arikan Y, et al. The effects of CO2 pneumoperitoneum on the apoptotic index in the peritoneum [J]. Adv Ther, 2007; 24(4): 883-889.
22.	Nesek-Adam V, Mrsic＇ V, Smiljanic＇ A, et al. Pathophysiologic effects of CO2-pneumoperitoneum in laparoscopic surgery [J]. Acta Med Croatica, 2007; 61(2): 165-170.
23.	Wildbrett P, Oh A, Naundorf D, et al. Impact of laparoscopic gases on peritoneal microenvironment and essential parameters of cell function [J]. Surg Endosc, 2003; 17(1): 78-82.
24.	Kim ZG, Mehl C, Lorenz M, et al. Impact of laparoscopic CO2-insufflation on tumor-associated molecules in cultured colorectal cancer cells [J]. Surg Endosc, 2002; 16(8): 1182-1186.
25.	Braga M, Frasson M, Vignali A, et al. Laparoscopic vs. open colectomy in cancer patients: long-term complications, quality of life, and survival [J]. Dis Colon Rectum, 2005; 48(12): 2217-2223.
26.	Kitano S, Shiraishi N, Uyama I, et al. A multicenter study on oncologic outcome of laparoscopic gastrectomy for early cancer in Japan [J]. Ann Surg, 2007; 245(1): 68-72.

1. Metzelder M, Kuebler JF, Shimotakahara A, et al. CO2 pneumoperitoneum increases survival in mice with polymicrobial peritonitis [J]. Eur J Pediatr Surg， 2008; 18(3): 171-175.
2. 余佩武，郝迎学. CO2气腹对胃肠道肿瘤侵袭转移影响的研究现状与进展 [J]. 中国普外基础与临床杂志, 2009; 16(10): 777-780.
3. Memon MA, Khan S, Yunus RM, et al. Meta-analysis of laparoscopic and open distal gastrectomy for gastric carcinoma [J]. Surg Endosc, 2008; 22(8): 1781-1789.
4. Topal B, Leys E, Ectors N, et al. Determinants of complications and adequacy of surgical resection in laparoscopic versus open total gastrectomy for adenocarcinoma [J]. Surg Endosc, 2008; 22(4): 980-984.
5. Person B, Cera SM. Prolonged postlaparoscopy carbon dioxide pneumoperitoneum [J]. Surg Laparosc Endosc Percutan Tech, 2008; 18(1): 114-117.
6. Fuganti PE, Rodrigues Júnior AJ, Rodrigues CJ, et al. A comparison of the effects of pneumoperitoneum and laparotomy on natural killer cell mediated cytotoxicity and Walker tumor growth in Wistar rats [J]. Surg Endosc, 2006; 20(12): 1858-1861.
7. Kosugi C, Ono M, Saito N, et al. Port site recurrence diagnosed by positron emission tomography after laparoscopic surgery for colon cancer [J]. Hepatogastroenterology, 2005; 52(65): 1440-1443.
8. Hirabayashi Y, Yamaguchi K, Shiraishi N, et al. Port-site metastasis after CO2 pneumoperitoneum: role of adhesion molecules and prevention with antiadhesion molecules [J]. Surg Endosc, 2004; 18(7): 1113-1117.
9. 田永刚，许军，代文杰，等. 人工气腹对结肠癌细胞增殖影响的实验研究 [J]. 中华普通外科杂志, 2007; 22(8): 623-626.
10. 马君俊，冯波，张轶，等. CO2气腹对结肠癌细胞侵袭能力影响的体外研究 [J]. 中国普外基础与临床杂志, 2007; 14(5): 512-515.
11. Hao YX, Zhong H, Zhang C, et al. Effects of simulated carbon dioxide and helium peumoperitoneum on proliferation and apoptosis of gastric cancer cells [J]. World J Gastroenterol, 2008; 14(14): 2241-2245.
12. Tan BJ, Dy JS, Chiu PY, et al. Effects of pneumoperitoneal gases and pressures on transitional cell carcinoma adhesion, growth, apoptosis and necrosis: an in vitro study [J]. J Urol, 2005; 174(4 Pt 1): 1463-1467.
13. Peng Y, Zheng M, Feng B, et al. Hyperthermic CO2 pneumoperitoneum induces apoptosis in human colon cancer cells through Bax-associated mitochondrial pathway [J]. Oncol Rep, 2008; 19(1): 73-79.
14. 王广义，孟宪瑛，顾建华，等. CO2气腹对肿瘤细胞种植与生长的影响 [J]. 中国普外基础与临床杂志, 2004; 11(2): 133-135.
15. Ordemann J, Hflich C, Braumann C, et al. Impact of pneumoperitoneum on expression of E-cadherin, CD44v6 and CD54 (ICAM-1) on HT-29 colon-carcinoma cells [J]. Zentralbl Chir, 2005; 130(5): 405-409.
16. Yavuz Y, Rnning K, Lyng O, et al. Effect of carbon dioxide pneumoperitoneum on tissue blood flow in the peritoneum, rectus abdominis, and diaphragm muscles [J]. Surg Endosc, 2003; 17(10): 1632-1635.
17. Schmidt AI, Reismann M, Kübler JF, et al. Exposure to carbon dioxide and helium reduces in vitro proliferation of pediatric tumor cells [J]. Pediatr Surg Int, 2006; 22(1): 72-77.
18. Ishida H, Hashimoto D, Nakada H, et al. Increased insufflation pressure enhances the development of liver metastasis in a mouse laparoscopy model: possible mechanisms [J]. Surg Endosc, 2002; 16(2): 331-335.
19. 张浩波，王灏，郑民华. 持续性CO2气腹影响腹腔镜手术切口肿瘤细胞种植的实验研究 [J]. 中国普外基础与临床杂志, 2002; 9(1): 16-18.
20. 石彦，郝迎学，雷晓，等. 模拟CO2气腹对人胃癌MKN-45细胞增殖和细胞周期的影响 [J]. 第三军医大学学报, 2007; 29(23): 2274-2276.
21. Tosun M, Samli H, Arikan Y, et al. The effects of CO2 pneumoperitoneum on the apoptotic index in the peritoneum [J]. Adv Ther, 2007; 24(4): 883-889.
22. Nesek-Adam V, Mrsic＇ V, Smiljanic＇ A, et al. Pathophysiologic effects of CO2-pneumoperitoneum in laparoscopic surgery [J]. Acta Med Croatica, 2007; 61(2): 165-170.
23. Wildbrett P, Oh A, Naundorf D, et al. Impact of laparoscopic gases on peritoneal microenvironment and essential parameters of cell function [J]. Surg Endosc, 2003; 17(1): 78-82.
24. Kim ZG, Mehl C, Lorenz M, et al. Impact of laparoscopic CO2-insufflation on tumor-associated molecules in cultured colorectal cancer cells [J]. Surg Endosc, 2002; 16(8): 1182-1186.
25. Braga M, Frasson M, Vignali A, et al. Laparoscopic vs. open colectomy in cancer patients: long-term complications, quality of life, and survival [J]. Dis Colon Rectum, 2005; 48(12): 2217-2223.
26. Kitano S, Shiraishi N, Uyama I, et al. A multicenter study on oncologic outcome of laparoscopic gastrectomy for early cancer in Japan [J]. Ann Surg, 2007; 245(1): 68-72.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Influence of Different Pressures and Time of CO2 Pneumoperitoneum on Adhesive and Invasive Ability of Gastric Cancer Cells

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