Study on Significance and Expression of Transforming Growth Factor α and β1 after 90% Portal Vein Ligation in Rats_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

YAO Yutong ¹ , LI Kezhou ¹ , WU Xiaoling ² , LUO Le ¹ , LUO Zhulin ¹ , YAN Hongtao ¹ , WANG Hua ¹ , TIAN Fuzhou ¹

1.General Surgery Center of PLA, General Hospital of Chengdu Military Region, Chengdu 610083, China;;
2.Department of Gastroenterology, General Hospital of Chengdu Military Region, Chengdu 610083, China;

Corresponding author：

Keywords：

Liver regeneration; Portal vein; Ligation; Transforming growth factor -α; Transforming growth factor -β1

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Abstract Full text Figures/Tables Video References Cited by

Objective To investigate the expressions of transforming growth factor (TGF) -α and -β1 after 90% portal branch ligation (PBL) in rats.
Methods Ninety-six SD rats were randomly divided into sham operation group and portal vein branches ligation group. The weight of both ligated and unligated lobes of liver were measured on 0.5, 1, 3, 5, 7, 14, 21, and 28 d after operation. The morphological changes of the unligated liver lobes were observed by microscope. The expressions of proliferating cell nuclear antigen (PCNA), TGF-α, and TGF-β1 of the unligated liver lobes were detected by immunohistochemistry.
Results After the ligation of 90% portal vein branches, hepatic lobe at the ligated side diminished progressively after ligation, whereas the lobes of the unligated side underwent compensatory regeneration. The ratio of unligated lobes weight to the whole liver increased slowly within 1 d, speeded up significantly during 1-5 d period, increased slowly on 5 d, and reached plateau phase on 7 d after operation. The expressions of PCNA protein markedly increased within 0.5-3 d (P lt;0.01), which reached the peak on 5 d and decreased slightly on 7 d after operation, but still higher than sham operation group level, and then gradually returned to the level of sham operation group lately. The expressions of TGF-α and TGF-β1 in the unligated liver lobes markedly increased on 0.5 d, and reached the peak on 3 d and 1 d respectively， and then gradually returned to the level of sham operation group in 7-28 d after operation.
Conclusion Ligation of 90% portal branches can induce active regeneration of unligated liver lobes in rats, whose initiation and proliferation are involved in the expressions of TGF-α and TGF-β1 protein.

Citation： YAO Yutong ,LI Kezhou,WU Xiaoling,LUO Le,LUO Zhulin,YAN Hongtao,WANG Hua,TIAN Fuzhou. Study on Significance and Expression of Transforming Growth Factor α and β1 after 90% Portal Vein Ligation in Rats. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2010, 17(7): 719-724. doi: Copy

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14.	Arakawa Y, Shimada M, Uchiyama H, et al. Beneficial effects of splenectomy on massive hepatectomy model in rats [J]. Hepatol Res, 2009; 39(4): 391397.
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1. Michalopoulos GK. Liver regeneration after partial hepatectomy: critical analysis of mechanistic dilemmas [J]. Am J Pathol, 2010; 176(1): 213.
2. Fausto N, Campbell JS, Riehle KJ. Liver regeneration [J]. Hepatology, 2006; 43(2 Suppl 1): S45S53.
3. Zou Q, Zhong Y, Su H, et al. Enhancement of humoral and cellular responses to HBsAg DNA vaccination by immunization with praziquantel through inhibition TGFβ/Smad2, 3 signaling [J]. Vaccine, 2010; 28(8): 20322038.
4. 王春雷, 黄志强, 万远廉, 等. TGFβ1诱导人肝癌细胞系凋亡信号转导机理的研究 [J]. 中国普外基础与临床杂志, 2005; 12(5): 480482, 487.
5. Yingchuan F, Chuntao L, Hui C, et al. Increased expression of TGFβ1 and Smad 4 on oxygeninduced retinopathy in neonatal mice [J]. Adv Exp Med Biol, 2010； 664(1): 7177.
6. Gaub J, Iversen J. Rat liver regeneration after 90% partial hepatectomy [J]. Hepatology, 1984; 4(5): 902904.
7. 郭美祥, 高丽华, 刘力波, 等. 大鼠门静脉分支结扎后肝再生的实验研究 [J]. 中国普外基础与临床杂志, 2006; 13(5): 550554.
8. Furrer K, Tian Y, Pfammatter T, et al. Selective portal vein embolization and ligation trigger different regenerative responses in the rat liver [J]. Hepatology, 2008; 47(5): 16151623.
9. Kucuktulu U, Alhan E, Tekelioglu Y, et al. The effects of pentoxifylline on liver regeneration after portal vein ligation in rats [J]. Liver Int, 2007; 27(2): 274279.
10. Hatsuno T, Kaneko T, Inoue S, et al. Changes in hepatic lobe volume in hepatocellular carcinoma after transcatheter arterial and percutaneous transhepatic portal embolization [J]. Hepatogastroenterology, 2004; 51(60): 18201824.
11. Corrêa Mde P, Ferreira AP, Gollner AM, et al. Markers expression of cell proliferation and apoptosis in basal cell carcinoma [J]. An Bras Dermatol, 2009; 84(6): 606614.
12. 龚加庆, 方驰华. 大鼠肝癌发生发展过程中肝组织增殖细胞核抗原动态演变规律 [J]. 中国普外基础与临床杂志, 2007; 14(1): 1114.
13. Taub R. Liver regeneration: from myth to mechanism [J]. Nat Rev Mol Cell Biol, 2004; 5(10): 836847.
14. Arakawa Y, Shimada M, Uchiyama H, et al. Beneficial effects of splenectomy on massive hepatectomy model in rats [J]. Hepatol Res, 2009; 39(4): 391397.
15. Michalopoulos GK. Liver regeneration [J]. J Cell Physiol, 2007; 213(2): 286300.
16. Zimmermann A. Regulation of liver regeneration [J]. Nephrol Dial Transplant, 2004; 19 Suppl 4: iv6iv10.
17. Mangnall D, Bird NC, Majeed AW. The molecular physiology of liver regeneration following partial hepatectomy [J]. Liver Int, 2003; 23(2):124138.
18. Khan AZ, Mudan SS. Liver regeneration: mechanisms, mysteries and more [J]. ANZ J Surg, 2007; 77(12): 914.
19. Du SS, Zeng ZC, Tang ZY, et al. Regenerative capacity of normal and irradiated liver following partial hepatectomy in rats [J]. Int J Radiat Biol, 2009; 85(12): 11141125.
20. Breitkopf K, Godoy P, Ciuclan L, et al. TGF /Smad signaling in the injured liver [J]. Z Gastroenterol, 2006; 44(1): 5766.
21. Mishra L, Banker T, Murray J, et al. Liver stem cells and hepatocellular carcinoma [J]. Hepatology, 2009; 49(1): 318329.
22. Palmes D, Zibert A, Budny T, et al. Impact of rapamycin on liver regeneration [J]. Virchows Arch, 2008; 452(5): 545557.
23. Koniaris LG, McKillop IH, Schwartz SI , et al. Liver regeneration [J]. J Am Coll Surg, 2003; 197(4): 634659.
24. Nguyen LN, Furuya MH, Wolfraim LA, et al. Transforming growth factorβ differentially regulates oval cell and hepatocyte proliferation [J]. Hepatology, 2007; 45(1): 3141.
25. Oe S, Lemmer ER, Conner EA, et al. Intact signaling by transforming growth factor β is not required for termination of liver regeneration in mice [J]. Hepatology, 2004; 40(5): 10981105.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Study on Significance and Expression of Transforming Growth Factor α and β1 after 90% Portal Vein Ligation in Rats

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