Protective Effect of Resveratrol on Intestinal Mucosal Ischemia-Reperfusion Injury in Rats with Severe Acute Pancreatitis_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

QIN Yong ¹ , DANG Xiaoyan ²

1.The Second Department of General Surgery， Xi’an Central Hospital of Xi’an City, Xi’an 710004, China;;
2.Department of Emergency, The Second Hospital of Xi’an Jiaotong University, Xi’an 710004, ChinaCorresponding Author: QIN Yong, E-mail: qingyong2@126.com;

Corresponding author：

Keywords：

Severe acute pancreatitis; Resveratrol; Intestinal mucosal ischemia-reperfusion injury; Oxygen free radical

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Objective 　To observe the influence of resveratrol on superoxide dismutase (SOD), malondialdehyde (MDA), intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) of intestinal mucosal ischemia-reperfusion injury protection in rats with severe acute pancreatitis (SAP).
Methods 　Fifty-four rats were divided into three groups randomly: sham operation group (SO group), SAP model group (SAP group) and resveratrol-treated group (Res group). SAP model was made by injecting sodium taurocholate 50 mg/kg to pancreatic bile duct and resveratrol was given intravenously at 5 min after inducing SAP model. The rats were sacrificed at 3 h, 6 h and 12 h after inducing SAP model respectively by equal number. The levels of MDA, SOD, ICAM-1 and VCAM-1 and histological changes of small intestine were measured.
Results 　The level of MDA in small intestine tissue in SAP group was significantly higher than that in SO group (P＜0.05), while the activity of SOD was significantly lower in the relevant tissues (P＜0.05). The expressions of ICAM-1 and VCAM-1 in SAP group were higher than those of SO group (P＜0.05). The activity of SOD in small intestine tissue in Res group was significantly higher than that in SAP group (P＜0.05); while the level of MDA was significantly lower in the relevant tissues (P＜0.05). The expressions of ICAM-1 and VCAM-1 in Res group were lower than those of SAP group (P＜0.05).
Conclusions 　Oxygen free radicals are concerned with the process of pathological changes in intestinal mucosal ischemia-reperfusion in rats with SAP. Resveratrol might increase SOD activity and decrease MDA level to attenuate lipid peroxidation in small intestine of SAP, and reduce the expressions of ICAM-1 and VCAM-1 in intestine, thus diminish the damage of the intestine in SAP. And it acts as a protective effect to small intestinal ischemia-reperfusion injury.

Citation： QIN Yong,DANG Xiaoyan. Protective Effect of Resveratrol on Intestinal Mucosal Ischemia-Reperfusion Injury in Rats with Severe Acute Pancreatitis. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2009, 16(10): 819-823. doi: Copy

1.	任雷, 马清涌, 王铮, 等. 白藜芦醇治疗大鼠急性胰腺炎肺损伤的实验研究 [Ｊ]. 西安交通大学学报(医学版), 2006; 27(3): 264-270.
2.	王连才, 马清涌, 沙焕臣, 等. 白藜芦醇对重症急性胰腺炎钙离子调节失衡的影响 [Ｊ].中华普通外科杂志, 2005; 20(12): 809-810.
3.	Ma ZH, Ma QY, Wang LC, et al. Effect of resveratrol on peritoneal macrophages in rats with severe acute pancreatitis [Ｊ]. Inflamm Res, 2005; 54(12): 522-527.
4.	Haraldsen P, Sun ZW, Brjesson A, et al. Multimodal management-of value in fulminant acute pancreatitis? [Ｊ]. Pancreatology, 2003; 3(1): 14-25.
5.	Saito I, Hashimoto S, Saluja A, et al. Intracellular transport of pancreatic zymogens during caerulein supramaximal stimulation [Ｊ]. Am J Physiol, 1987; 253(4 Pt 1): G517-G526.
6.	武金宝, 王继德, 张亚历. 肠黏膜屏障研究进展 [Ｊ]. 世界华人消化杂志, 2003; 11(5): 619-623.
7.	Meng Y, Zhang M, Xu J, et al. Effect of resveratrol on microcirculation disorder and lung injury following severe acute pancreatitis in rats [Ｊ]. World J Gastroenterol, 2005; 11(3): 433-435.
8.	李震东, 马清涌, 徐军, 等. Fas和FasL在急性胰腺炎中的共表达及其与凋亡的关系 [Ｊ]. 南方医科大学学报, 2006; 26(1): 25-29.
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12.	刁永鹏，陈宏，李非. 重症急性胰腺炎肠屏障功能障碍的研究进展 [Ｊ]. 中国普外基础与临床杂志, 2009; 16(9): 588-592.
13.	冯永红, 许实波. 白藜芦醇药理作用研究进展 [Ｊ]. 国外医药植物药分册, 1996; 11(4): 155-157.
14.	Bhat KPL, Kosmeder JW 2nd, Pezzuto JM. Biological effects of resveratrol [Ｊ]. Antioxid Redox Signal, 2001; 3(6): 1041-1064.
15.	Baolin L, Inami Y, Tanaka H, et al. Resveratrol inhibits the release of mediators from bone marrow-derived mouse mast cells in vitro [Ｊ]. Planta Med, 2004; 70(4): 305-309.
16.	Kawada N, Seki S, Inoue M, et al. Effect of antioxidants, resveratrol, quercetin, and N-acetylcysteine, on the functions of cultured rat hepatic stellate cells and Kupffer cells [Ｊ]. Hepatology, 1998; 27(5): 1265-1274.
17.	De Salvia R, Festa F, Ricordy R, et al. Resveratrol affects in a different way primary versus fixed DNA damage induced by H2O2 in mammalian cells in vitro [Ｊ]. Toxicol Lett, 2002; 135(1-2): 1-9.
18.	吴浩, 陈晓理, 邓一芸, 等. 生长抑素对急性胰腺炎大鼠血中IL-10和TGF-β1的影响及意义 [Ｊ]. 四川大学学报(医学版), 2003; 34(2): 315-317.
19.	Schmeling DJ, Caty MG, Oldham KT, et al. Evidence for neutrophil-related acute lung injury after intestinal ischemia-reperfusion [Ｊ]. Surgery, 1989; 106(2): 195-201.
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21.	彭晓辉, 鲜于剑波, 高宏凯, 等. 急性胰腺炎胰腺内源性RAS的活化与局部微循环P选择素表达变化规律的研究 [Ｊ]. 华西医学, 2003; 18(4): 465-466.
22.	吕云福主编. 现代胰腺外科学 [M]. 北京: 人民军医出版社, 2003: 129.
23.	Harlan JM. Leukocyte-endothelial interactions [Ｊ]. Blood, 1985; 65(3): 513-525.
24.	单秋玲. 细胞粘附在缺血再灌注损伤中的作用 [Ｊ]. 解放军预防医学杂志, 1999; 17(2): 148-152.
25.	Schoenberg MH, Poch B, Younes M, et al. Involvement of neutrophils in postischaemic damage to the small intestine [Ｊ]. Gut, 1991; 32(8): 905-912.
26.	罗昆仑. 中性粒细胞介导组织损伤的机理 [Ｊ]. 国外医学生理病理科学与临床分册, 1999; 19(1): 51-54.

1. 任雷, 马清涌, 王铮, 等. 白藜芦醇治疗大鼠急性胰腺炎肺损伤的实验研究 [Ｊ]. 西安交通大学学报(医学版), 2006; 27(3): 264-270.
2. 王连才, 马清涌, 沙焕臣, 等. 白藜芦醇对重症急性胰腺炎钙离子调节失衡的影响 [Ｊ].中华普通外科杂志, 2005; 20(12): 809-810.
3. Ma ZH, Ma QY, Wang LC, et al. Effect of resveratrol on peritoneal macrophages in rats with severe acute pancreatitis [Ｊ]. Inflamm Res, 2005; 54(12): 522-527.
4. Haraldsen P, Sun ZW, Brjesson A, et al. Multimodal management-of value in fulminant acute pancreatitis? [Ｊ]. Pancreatology, 2003; 3(1): 14-25.
5. Saito I, Hashimoto S, Saluja A, et al. Intracellular transport of pancreatic zymogens during caerulein supramaximal stimulation [Ｊ]. Am J Physiol, 1987; 253(4 Pt 1): G517-G526.
6. 武金宝, 王继德, 张亚历. 肠黏膜屏障研究进展 [Ｊ]. 世界华人消化杂志, 2003; 11(5): 619-623.
7. Meng Y, Zhang M, Xu J, et al. Effect of resveratrol on microcirculation disorder and lung injury following severe acute pancreatitis in rats [Ｊ]. World J Gastroenterol, 2005; 11(3): 433-435.
8. 李震东, 马清涌, 徐军, 等. Fas和FasL在急性胰腺炎中的共表达及其与凋亡的关系 [Ｊ]. 南方医科大学学报, 2006; 26(1): 25-29.
9. 张肇达, 严律南, 刘续宝主编. 急性胰腺炎 [M]. 北京: 人民卫生出版社, 2004: 168-169.
10. Granell S, Gironella M, Bulbena O, et al. Heparin mobilizes xanthine oxidase and induces lung inflammation in acute pancreatitis [Ｊ]. Crit Care Med, 2003; 31(2): 525-530.
11. Uruuela A, Sevillano S, de la Mano AM, et al. Time-course of oxygen free radical production in acinar cells during acute pancreatitis induced by pancreatic duct obstruction [Ｊ]. Biochim Biophys Acta, 2002; 1588(2): 159-164.
12. 刁永鹏，陈宏，李非. 重症急性胰腺炎肠屏障功能障碍的研究进展 [Ｊ]. 中国普外基础与临床杂志, 2009; 16(9): 588-592.
13. 冯永红, 许实波. 白藜芦醇药理作用研究进展 [Ｊ]. 国外医药植物药分册, 1996; 11(4): 155-157.
14. Bhat KPL, Kosmeder JW 2nd, Pezzuto JM. Biological effects of resveratrol [Ｊ]. Antioxid Redox Signal, 2001; 3(6): 1041-1064.
15. Baolin L, Inami Y, Tanaka H, et al. Resveratrol inhibits the release of mediators from bone marrow-derived mouse mast cells in vitro [Ｊ]. Planta Med, 2004; 70(4): 305-309.
16. Kawada N, Seki S, Inoue M, et al. Effect of antioxidants, resveratrol, quercetin, and N-acetylcysteine, on the functions of cultured rat hepatic stellate cells and Kupffer cells [Ｊ]. Hepatology, 1998; 27(5): 1265-1274.
17. De Salvia R, Festa F, Ricordy R, et al. Resveratrol affects in a different way primary versus fixed DNA damage induced by H2O2 in mammalian cells in vitro [Ｊ]. Toxicol Lett, 2002; 135(1-2): 1-9.
18. 吴浩, 陈晓理, 邓一芸, 等. 生长抑素对急性胰腺炎大鼠血中IL-10和TGF-β1的影响及意义 [Ｊ]. 四川大学学报(医学版), 2003; 34(2): 315-317.
19. Schmeling DJ, Caty MG, Oldham KT, et al. Evidence for neutrophil-related acute lung injury after intestinal ischemia-reperfusion [Ｊ]. Surgery, 1989; 106(2): 195-201.
20. 蒋健主编．缺血再灌注损伤-危重病基础及临床研究新领域 [M]．北京：学苑出版社， 1997： 310.
21. 彭晓辉, 鲜于剑波, 高宏凯, 等. 急性胰腺炎胰腺内源性RAS的活化与局部微循环P选择素表达变化规律的研究 [Ｊ]. 华西医学, 2003; 18(4): 465-466.
22. 吕云福主编. 现代胰腺外科学 [M]. 北京: 人民军医出版社, 2003: 129.
23. Harlan JM. Leukocyte-endothelial interactions [Ｊ]. Blood, 1985; 65(3): 513-525.
24. 单秋玲. 细胞粘附在缺血再灌注损伤中的作用 [Ｊ]. 解放军预防医学杂志, 1999; 17(2): 148-152.
25. Schoenberg MH, Poch B, Younes M, et al. Involvement of neutrophils in postischaemic damage to the small intestine [Ｊ]. Gut, 1991; 32(8): 905-912.
26. 罗昆仑. 中性粒细胞介导组织损伤的机理 [Ｊ]. 国外医学生理病理科学与临床分册, 1999; 19(1): 51-54.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Protective Effect of Resveratrol on Intestinal Mucosal Ischemia-Reperfusion Injury in Rats with Severe Acute Pancreatitis

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