Effect of Quercetin onProliferation and Apoptosis of Human Breast Cancer Cell_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

LI Shizheng ¹ , LI Kun ² , ZHANG Junhua ¹ , LI Jincheng ¹ , SHAN Jixian. ³

1.Department of General Surgery, The First Affiliated Hospital of Liaoning Medical College, Jinzhou 121001, China;;
2.Department of Clinical Laboratory, The First Affiliated Hospital of Liaoning Medical College, Jinzhou 121001, China;;
3.Department of Surgical Oncology, The First Affiliated Hospital of China Medical University, Shenyang 110001, ChinaCorresponding Author: LI Shi-zheng, E-mail: ychzon@yahoo.com.cn;

Corresponding author：

Keywords：

Quercetin; Breast cancer; Proliferation; Apoptosis; Cell cycle; Caspase-3

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Objective To investigate the effect of quercetin on human breast cancer cells and related mechanism. MethodsHuman breast cancer cell line MDA-MB-435S was treated by different concentrations of quercetin (12.5, 25, 50, 100 and 200 μmol/L). The inhibition ratio of human breast cancer cell was measured by trypan blue dye exclusion test, the proliferation cycle of human breast cancer cell was analyzed by flow cytometry and the expression of caspase-3 mRNA was determined by RT-PCR. ResultsQuercetin could inhibit the proliferation of breast cancer cell in the dose- and time-dependent manner. Quercetin could change the cell cycle of breast cancer cell, with the concentrations of quercetin increasing, the percentages of breast cancer cell at G0/G1 phase decreased, which increased at S phase, and increased at G2/M phase followed decrease. Quercetin could activate the expression of caspase-3 mRNA in breast cancer cell. ConclusionQuercetin can inhibit proliferation and induce apoptosis of human breast cancer cell, the mechanism may be associated with the change of cell cycle and the upregulation of caspase-3 expression.

Citation： LI Shizheng,LI Kun,ZHANG Junhua,LI Jincheng,SHAN Jixian.. Effect of Quercetin onProliferation and Apoptosis of Human Breast Cancer Cell. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2009, 16(2): 124-128. doi: Copy

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15.	Sarkar FH, Li Y. Mechanisms of cancer chemoprevention by soy isoflavone genistein [Ｊ]. Cancer Metastasis Rev, 2002; 21 (3-4)： 265.
16.	Li Z, Li J, Mo B, et al. Genistein induces G2/M cell cycle arrest via stable activation of ERK1/2 pathway in MDA-MB-231 breast cancer cells [Ｊ]. Cell Biol Toxicol, 2008; 24(5)： 401.
17.	Conklin CM, Bechberger JF, MacFabe D, et al. Genistein and quercetin increase connexin43 and suppress growth of breast cancer cells [Ｊ]. Carcinogenesis, 2007; 28(1)： 93.
18.	Shao ZM, Shen ZZ, Fontana JA, et al. Genistein’s “ER-dependent and independent” actions are mediated through ER pathways in ER-positive breast carcinoma cell lines [Ｊ]. Anticancer Res, 2000; 20(4)：2409.
19.	Vissac-Sabatier C, Bignon YJ, Bernard-Gallon DJ. Effects of the phytoestrogens genistein and daidzein on BRCA2 tumor suppressor gene expression in breast cell lines [Ｊ]. Nutr Cancer, 2003; 45(2)： 247.
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1. Hollman PC, Katan MB. Dietary flavonoids: intake, health effects and bioavailability [Ｊ]. Food Chem Toxicol, 1999; 37(9-10)： 937.
2. IWase Y, Takemura Y, Ju-ichi M, et al. Inhibitory effect of flavonoid derivatives on Epstein-Barr virus activation and two-stage carcinogenesis of skin tumors [Ｊ]. Cancer Lett, 2001; 173(2)： 105.
3. Kim WK, Bang MH, Kim ES, et al. Quercetin decreases the expression of ErbB2 and ErbB3 proteins in HT-29 human colon cancer cells [Ｊ]. J Nutr Biochem, 2005; 16(3)： 155.
4. Yang JH, Hsia TC, Kuo HM, et al. Inhibition of lung cancer cell growth by quercetin glucuronides via G2/M arrest and induction of apoptosis[Ｊ]. Drug Metab Dispos, 2006; 34(2)： 296.
5. 沈丽霞，徐惠玉，赵丕文，等.槲皮素对人类乳腺癌细胞增殖的影响[Ｊ]. 中国中医药信息杂志， 2008； 15(3)： 30.
6. 柯尊金，丁心喜，董文奎，等.槲皮素对人膀胱癌BIU-87细胞增殖和凋亡的影响[Ｊ].实用癌症杂志， 2008； 23(2)： 116.
7. Hartwell LH, Kastan MB. Cell cycle control and cancer [Ｊ]. Science, 1994; 266(5192)： 1821.
8. Hartwell LH, Weinert TA. Checkpoints: controls that ensure the order of cell cycle events [Ｊ]. Science, 1989; 246(4930)： 629.
9. Chiarugi V, Magnelli L, Cinelli M, et al. Apoptosis and the cell cycle [Ｊ]. Cell Mol Biol Res, 1994; 40(7-8)： 603.
10. 王玉，孙黎光，夏春辉.Caspase介导的Fas凋亡途径 [Ｊ].世界华人消化杂志， 2006； 14(36)： 3439.
11. 金琳芳，陈同钰.Bcl-2基因蛋白家族与细胞凋亡[Ｊ].医学综述， 2005； 11(5)： 446.
12. 马银斌，吴诚义，陈鑫.As2O3对人乳腺癌中NF-κB p65、survivin和caspase-3的作用及相关性研究[Ｊ].中国普外基础与临床杂志， 2006； 13(4)：409.
13. 闻春生，应斌武，张永刚，等. 槲皮素对肺腺癌细胞株A549细胞中凋亡相关因子caspase-3表达的影响[Ｊ].中国肺癌杂志， 2008； 11(2)：194.
14. van der Woude H, Gliszczyńska-Swigo A, Struijs K, et al. Biphasic modulation of cell proliferation by quercetin at concentrations physiologically relevant in humans [Ｊ]. Cancer Lett, 2003; 200(1)： 41.
15. Sarkar FH, Li Y. Mechanisms of cancer chemoprevention by soy isoflavone genistein [Ｊ]. Cancer Metastasis Rev, 2002; 21 (3-4)： 265.
16. Li Z, Li J, Mo B, et al. Genistein induces G2/M cell cycle arrest via stable activation of ERK1/2 pathway in MDA-MB-231 breast cancer cells [Ｊ]. Cell Biol Toxicol, 2008; 24(5)： 401.
17. Conklin CM, Bechberger JF, MacFabe D, et al. Genistein and quercetin increase connexin43 and suppress growth of breast cancer cells [Ｊ]. Carcinogenesis, 2007; 28(1)： 93.
18. Shao ZM, Shen ZZ, Fontana JA, et al. Genistein’s “ER-dependent and independent” actions are mediated through ER pathways in ER-positive breast carcinoma cell lines [Ｊ]. Anticancer Res, 2000; 20(4)：2409.
19. Vissac-Sabatier C, Bignon YJ, Bernard-Gallon DJ. Effects of the phytoestrogens genistein and daidzein on BRCA2 tumor suppressor gene expression in breast cell lines [Ｊ]. Nutr Cancer, 2003; 45(2)： 247.
20. Kousidou OC, Mitropoulou TN, Roussidis AE, et al. Genistein suppresses the invasive potential of human breast cancer cells through transcriptional regulation of metalloproteinases and their tissue inhibitors [Ｊ]. Int J Oncol, 2005; 26(4)： 1101.
21. Lim HA, Kim JH, Kim JH, et al. Genistein induces glucose-regulated protein 78 in mammary tumor cell [Ｊ]. J Med Food, 2006; 9(1)： 28.
22. Akbas SH, Timur M, Ozben T. The effect of quercetin on topotecan cytotoxicity in MCF-7 and MDA-MB 231 human breast cancer cells [Ｊ]. J Surg Res, 2005; 125(1)：49.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effect of Quercetin onProliferation and Apoptosis of Human Breast Cancer Cell

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