Analysis of Lactate Dehydrogenase Activity and Its Isoenzyme Pattern in Pancreatic Normal Tissue, Carcinoma and Adjacent Non-Cancerous Tissue_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

ZHANG Shuhua , XIONG Jiongxin , YANG Zhiyong , WANG Chunyou

Center of Pancreatic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, ChinaCorresponding Author:WANG Chun-you, E-mail: chunyouwang52@126.com;

Corresponding author：

Keywords：

Lactate dehydrogenase; Isoenzymes; Pancreatic cancer

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Objective To detect the activity of lactate dehydrogenase (LDH) and LDH isoenzyme, and to explore the relation between biological behavior ofpancreatic cancer and glycolysis. MethodsConsecutive 12 cases of pancreatic ductal adenocarcinoma and 12 benign lesions such as insulinoma from October 2006 to July 2008 were collected, as well as normal pancreatic tissues. The total activity of the LDH was detected by the LDH testing kits, and the iosenzyme pattern of LDH was inspected by the France Sebia hydrasys. ResultsCompared to the normal tissue, LDH activity ofpancreatic cancer and adjacent non-cancerous tissue was significantly higher (P＜0.05). LDH iosenzyme pattern in cancer tissue was also significantly different, the percentage of LDH4 and LDH5 increased obviously, and were greater than that innormal tissue (P＜0.05). ConclusionThe alteration of LDH activity and its isoenzyme pattern are possibly related to the pathogenesis of pancreatic cancer. Inhibit the LDH activity may be a new therapeutic strategy.

Citation： ZHANG Shuhua,XIONG Jiongxin,YANG Zhiyong,WANG Chunyou. Analysis of Lactate Dehydrogenase Activity and Its Isoenzyme Pattern in Pancreatic Normal Tissue, Carcinoma and Adjacent Non-Cancerous Tissue. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2009, 16(2): 129-132. doi: Copy

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4.	韩明勇，王家林，郭其森，等.血清乳酸脱氢酶水平对肺癌诊断意义的研究[Ｊ].肿瘤防治杂志， 2003； 10(4)：364.
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13.	赵玉沛，戴梦华.胰腺癌生物治疗现状与进展[Ｊ]. 中国普外基础与临床杂志， 2002； 9(6)：371.
14.	赵玉沛.胰腺癌诊治现状与展望[Ｊ].中国普外基础与临床杂志， 2003； 10(3)：205.
15.	Kroemer G. Mitochondria in cancer[Ｊ]. Oncogene, 2006; 25(34)：4630.
16.	Bartrons R, Caro J. Hypoxia, glucose metabolism and the Warburg’s effect[Ｊ]. J Bioenerg Biomembr, 2007; 39(3)：223.
17.	Pedersen PL. Warburg, me and Hexokinase 2: Multiple discoveries of key molecular events underlying one of cancers’ most common phenotypes, the “Warburg Effect”, i.e., elevated glycolysis in the presence of oxygen [Ｊ]. J Bioenerg Biomembr, 2007; 39(3)：211.
18.	Chen Z, Lu W, Garcia-Prieto C, et al. The Warburg effect and its cancer therapeutic implications [Ｊ]. J Bioenerg Biomembr, 2007; 39(3)：267.
19.	Garber K. Energy deregulation: licensing tumors to grow [Ｊ]. Science, 2006; 312(5777)：1158.
20.	Pelicano H, Martin DS, Xu RH, et al. Glycolysis inhibition for anticancer treatment [Ｊ]. Oncogene, 2006; 25(34)：4633.
21.	邹满意，陆伟. 糖酵解酶抑制剂抗肿瘤作用的研究进展[Ｊ].武警医学院学报， 2006； 15(3)：286.

1. Hsu PP, Sabatini DM. Cancer cell metabolism: Warburg and beyond [Ｊ]. Cell, 2008; 134(5)：703.
2. Deberardinis RJ. Is cancer a disease of abnormal cellular metabolism? New angles on an old idea [Ｊ]. Genet Med, 2008; 10(11)：767.
3. Deberardinis RJ, Sayed N, Ditsworth D, et al. Brick and brick: metabolism and tumor cell growth [Ｊ]. Curr Opin Genet Dev, 2008; 18(1)：54.
4. 韩明勇，王家林，郭其森，等.血清乳酸脱氢酶水平对肺癌诊断意义的研究[Ｊ].肿瘤防治杂志， 2003； 10(4)：364.
5. Ugurel S. Serum markers for melanoma [Ｊ]. Hautarzt, 2005; 56(2)：173.
6. Boran N, Kayikioˇglu F, Yalva S, et al. Significance of serum and peritoneal fluid lactate dehydrogenase levels in ovarian cancer [Ｊ]. Gynecol Obstet Invest, 2000; 49(4)：272.
7. Suh SY, Ahn HY. Lactate dehydrogenase as a prognostic factor for survival time of terminally ill cancer patients: a preliminary study[Ｊ]. Eur J Cancer, 2007; 43(6)：1051.
8. Naruse K, Yamada Y, Aoki S, et al. Lactate dehydrogenase is a prognostic indicator for prostate cancer patients with bone metastasis [Ｊ]. Hinyokika Kiyo, 2007; 53(5)：287.
9. Turen S, Ozyar E, Altundag K, et al. Serum lactate dehydrogenase level is a prognostic factor in patients with locoregionally advanced nasopharyngeal carcinoma treated with chemoradiotherapy [Ｊ].Cancer Invest, 2007; 25(5)：315.
10. Shaw RJ. Glucose metabolism and cancer [Ｊ]. Curr Opin Cell Biol, 2006; 18(6)：598.
11. Kondoh H. Cellular life span and the Warburg effect [Ｊ]. Exp Cell Res, 2008; 314(9)：1923.
12. Alcazar O, Tiedge M, Lenzen S. Importance of lactate dehydrogenase for the regulation of glycolytic flux and insulin secretion in insulin-producing cells[Ｊ]. Biochem J, 2000; 352(Pt2)：373.
13. 赵玉沛，戴梦华.胰腺癌生物治疗现状与进展[Ｊ]. 中国普外基础与临床杂志， 2002； 9(6)：371.
14. 赵玉沛.胰腺癌诊治现状与展望[Ｊ].中国普外基础与临床杂志， 2003； 10(3)：205.
15. Kroemer G. Mitochondria in cancer[Ｊ]. Oncogene, 2006; 25(34)：4630.
16. Bartrons R, Caro J. Hypoxia, glucose metabolism and the Warburg’s effect[Ｊ]. J Bioenerg Biomembr, 2007; 39(3)：223.
17. Pedersen PL. Warburg, me and Hexokinase 2: Multiple discoveries of key molecular events underlying one of cancers’ most common phenotypes, the “Warburg Effect”, i.e., elevated glycolysis in the presence of oxygen [Ｊ]. J Bioenerg Biomembr, 2007; 39(3)：211.
18. Chen Z, Lu W, Garcia-Prieto C, et al. The Warburg effect and its cancer therapeutic implications [Ｊ]. J Bioenerg Biomembr, 2007; 39(3)：267.
19. Garber K. Energy deregulation: licensing tumors to grow [Ｊ]. Science, 2006; 312(5777)：1158.
20. Pelicano H, Martin DS, Xu RH, et al. Glycolysis inhibition for anticancer treatment [Ｊ]. Oncogene, 2006; 25(34)：4633.
21. 邹满意，陆伟. 糖酵解酶抑制剂抗肿瘤作用的研究进展[Ｊ].武警医学院学报， 2006； 15(3)：286.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Analysis of Lactate Dehydrogenase Activity and Its Isoenzyme Pattern in Pancreatic Normal Tissue, Carcinoma and Adjacent Non-Cancerous Tissue

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