Significances and Expressions of Y14 and Upf1 in Human Breast Cancer Tissues and Normal Breast Tissues_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

RAN Liang ¹ , TU Gang ¹ , WANG Xiaoyi ¹ , ZHAO Cong ² , REN Guosheng ¹

1.Department of Three Gland Surgery， The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China;;
2.Department of Digestive Internal Medicine， The Second People’s Hospital of Chengdu City, Chengdu 610017, ChinaCorresponding Author: REN Guo-sheng， E-mail: rgs726@tom.com;

Corresponding author：

Keywords：

Nonsense-mediated mRNA decay; Y14; Upf1; Breast cancer tissue; Normal breast tissue

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Abstract Full text Figures/Tables Video References Cited by

Objective　 To study the significances and expressions of Y14 and Upf1 in human breast cancer tissues and human normal breast tissues.
Methods 　The expressions of Y14 and Upf1 were determined in human breast cancer tissues and human normal breast tissues by immunohistochemistry and laser scanning confocal microscope.
Results 　①Y14 and Upf1 levels of human breast cancer tissues were obviously higher than those of human normal breast tissues (P lt;0.05). ②Y14 and Upf1 levels of the class Ⅲ were obviously higher than those of the class Ⅰ and Ⅱ in breast cancer tissues (P lt;0.05). ③Y14 and Upf1 levels in breast cancer tissues of patients with positive axillary lymph nodes were obviously higher than those with negative axillary lymph nodes (P lt;0.05).
Conclusion 　The function of nonsense-mediated mRNA decay (NMD) is obviously enhanced in breast cancer tissues. Expressions of Y14 and Upf1 levels of the class Ⅲ cancer tissue and cancer with positive axillary lymph node are high because of NMD.

Citation： RAN Liang,TU Gang,WANG Xiaoyi,ZHAO Cong,REN Guosheng. Significances and Expressions of Y14 and Upf1 in Human Breast Cancer Tissues and Normal Breast Tissues. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2009, 16(4): 281-284. doi: Copy

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16.	敬静, 吕青, 李宏江, 等. 乳腺癌患者血清和肿瘤组织VEGF表达与临床预后的关系 [Ｊ]. 中国普外基础与临床杂志, 2006; 13(6): 705-708.
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1. Weischenfeldt J, Lykke-Andersen J, Porse B. Messenger RNA surveillance: neutralizing natural nonsense [Ｊ]. Curr Biol, 2005; 15(14): R559-R562.
2. Culbertson MR, Leeds PF. Looking at mRNA decay pathways through the window of molecular evolution [Ｊ]. Curr Opin Genet Dev, 2003; 13(2): 207-214.
3. Kunz JB, Neu-Yilik G, Hentze MW, et al. Functions of hUpf3a and hUpf3b in nonsense-mediated mRNA decay and translation [Ｊ]. RNA, 2006; 12(6): 1015-1022.
4. 杨梅，宋尔卫. 小分子RNA 与肿瘤治疗 [Ｊ]. 生命科学, 2008; 20(2): 213-217.
5. Holbrook JA, Neu-Yilik G, Hentze MW, et al. Nonsense-mediated decay approaches the clinic [Ｊ]. Nat Genet, 2004; 36(8): 801-808.
6. Lewis BP, Green RE, Brenner SE. Evidence for the widespread coupling of alternative splicing and nonsense-mediated mRNA decay in humans [Ｊ]. Proc Natl Acad Sci USA, 2003; 100(1): 189-192.
7. Maderazo AB, Belk JP, He F, et al. Nonsense-containing mRNAs that accumulate in the absence of a functional nonsense-mediated mRNA decay pathway are destabilized rapidly upon its restitution [Ｊ]. Mol Cell Biol, 2003; 23(3): 842-851.
8. Gehring NH, Kunz JB, Neu-Yilik G, et al. Exon-junction complex components specify distinct routes of nonsense-mediated mRNA decay with differential cofactor requirements [Ｊ]. Mol Cell, 2005; 20(1): 65-75.
9. Kashima I, Yamashita A, Izumi N, et al. Binding of a novel SMG-1-Upf1-eRF1-eRF3 complex (SURF) to the exon junction complex triggers Upf1 phosphorylation and nonsense-mediated mRNA decay [Ｊ]. Genes Dev, 2006; 20(3): 355-367.
10. Schell T, Kulozik AE, Hentze MW. Integration of splicing, transport and translation to achieve mRNA quality control by the nonsense-mediated decay pathway [Ｊ]. Genome Biol, 2002; 3(3): Reviews1006.1-1006.6.
11. 王德延，傅西林，潘帼利, 等. 乳腺癌组织学类型与预后的研究 [Ｊ]. 肿瘤临床， 1985; 12(3): 135-139.
12. 王良, 任国胜, 涂刚, 等. 外源性透明质酸酶对人乳腺癌细胞侵袭力及血管形成能力的影响 [Ｊ]. 中国普外基础与临床杂志, 2005; 12(3): 216-218.
13. 郝朗松，朱霞，钱昆，等. 乳腺癌组织中缺氧诱导因子-1α的表达及其与增殖细胞核抗原、微血管密度的关系 [Ｊ].中国普外基础与临床杂志, 2007; 14(2): 203-207.
14. 李树玲主编. 乳腺肿瘤学 [M]. 北京：科学技术文献出版社, 2000: 520.
15. 余应年. 癌基因／抑癌基因与细胞增殖控制 [Ｊ]. 实用肿瘤杂志, 1995; 10(1): 6-8.
16. 敬静, 吕青, 李宏江, 等. 乳腺癌患者血清和肿瘤组织VEGF表达与临床预后的关系 [Ｊ]. 中国普外基础与临床杂志, 2006; 13(6): 705-708.
17. Culbertson MR, Neeno-Eckwall E. Transcript selection and the recruitment of mRNA decay factors for NMD in Saccharomyces cerevisiae [Ｊ]. RNA, 2005; 11(9): 1333-1339.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Significances and Expressions of Y14 and Upf1 in Human Breast Cancer Tissues and Normal Breast Tissues

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