Influence of Colectomy on Expressions of 5-Hydroxy Tryptamine and Chromogranin A in Colon Mucosa of Chinchilla Rabbits_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

QIAN Zhiyuan ¹ , TU Xiaohuang ¹ , CHEN Zhiyao ¹ , FENG Changyin ²

1.Research Institute of General Surgery, Fuzhou General Hospital of Nanjing Military Command， Fuzhou 350025, China;;
2.Research Institute of Pathology, Fuzhou General Hospital of Nanjing Military Command， Fuzhou 350025, China;

Corresponding author：

Keywords：

Colectomy; Chinchilla rabbit; Colon mucosa; 5-hydroxy tryptamine; Chromogranin A

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Objective To investigate the influence of colectomy on the expressions of 5-hydroxy tryptamine (5-HT) and chromogranin A (CgA) in colon mucosa of Chinchilla rabbits. Methods Colon (7-8 cm) upon colon-rectum junction (control group) of 15 Chinchilla rabbits was cut out. After two weeks, these rabbits were executed and the samples of colon at anastomotic stoma (study group) were taken. 5-HT positive cells and CgA positive cells in two groups were detected by immunohistochemical method. Results The number of 5-HT positive cells was 10.40±2.22 in control group， and 26.27±2.35 in study group; the number of CgA positive cells was 20.60±5.34 in control group, and 51.51±6.13 in study group. There were significant differences between two groups respectively (P＜0.01). Conclusion The increase of 5-HT positive cells and CgA positive cells can be caused by colectomy.

Citation： QIAN Zhiyuan,TU Xiaohuang,CHEN Zhiyao,FENG Changyin. Influence of Colectomy on Expressions of 5-Hydroxy Tryptamine and Chromogranin A in Colon Mucosa of Chinchilla Rabbits. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2009, 16(6): 452-454. doi: Copy

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1. Ahlman H, Nilsson. The gut as the largest endocrine organ in the body [Ｊ]. Ann Oncol, 2001; 12 Suppl 2: S63-S68.
2. 涂小煌，陈志耀. 补中益气丸联合易蒙停治疗大肠癌术后腹泻疗效观察 [Ｊ]. 中国中西医结合杂志, 2008; 28(8): 738-741.
3. Watts SW. 5-HT in systemic hypertension: foe, friend or fantasy? [Ｊ]. Clin Sci (Lond), 2005; 108(5): 399-412.
4. Spiller R. Serotonin and GI clinical disorders [Ｊ]. Neuropharmacology, 2008; 55(6): 1072-1080.
5. Grundy D. 5-HT system in the gut: roles in the regulation of visceral sensitivity and motor functions [Ｊ]. Eur Rev Med Pharmacol Sci, 2008; 12 Suppl 1: 63-67.
6. Wiedenmann B, Huttner WB．Synaptophysin and chromogr-anins/secretogranins-widespread constituents of distinct types of neuroendocrine vesicles and new tools in tumor diagnosis [Ｊ]．Virchows Arch B Cell Pathol Incl Mol Pathol, 1989; 58(2): 95-121.
7. O’Connor DT, Wu H, Gill BM， et al. Hormone storage vesicle proteins. Transcriptional basis of the widespread neuroendocrine expression of chromogranin A, and evidence of its diverse biological actions, intracellular and extracellular [Ｊ]. Ann N Y Acad Sci, 1994; 733: 36-45.
8. Portela-Gomes GM, Stridsberg M. Chromogranin A in the human gastrointestinal tract: an immunocytochemical study with region-specific antibodies [Ｊ]. J Histochem Cytochem, 2002; 50(11): 1487-1492.
9. Schürmann G, Bishop AE, Facer P, et al. Secretoneurin: a new peptide in the human enteric nervous system [Ｊ]. Histochem Cell Biol, 1995; 104(1): 11-19.
10. Ghia JE, Pradaud I, Crenner F, et al. Effect of acetic acid or trypsin application on rat colonic motility in vitro and modulation by two synthetic fragments of chromogranin A [Ｊ]. Regul Pept, 2005; 124(1-3): 27-35.
11. Albuquerque JF, Ferra MA, Portela-Gomes GM. Adaptive changes of the enterochromaffin and gastrin cells in the rat gastrointestinal tract following subtotal colectomy [Ｊ]. Scand J Gastroenterol, 2006; 41(8): 963-968.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Influence of Colectomy on Expressions of 5-Hydroxy Tryptamine and Chromogranin A in Colon Mucosa of Chinchilla Rabbits

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