Study on Expressions and Significances of Endostatin, bFGF and CD34 in Gallbladder Cancer_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

WEN Decai ¹ , GENG Jianli ¹ , GAO Ruohui ¹ , GUO Renxuan ² , ZHANG Yong ³

1.Department of General Surgery No.2, Weihai Municipal Hospital, Weihai 264200, Shandong Province, China;;
2.Department of General Surgery No.2, The First Affiliated Hospital of China Medical University, Shenyang 110001, China;;
3.Department of Pathology, The First Affiliated Hospital of China Medical University, Shenyang 110001, China;

Corresponding author：

Keywords：

Gallbladder cancer; Endostatin; Basic fibroblast growth factor; CD34; Microvessel density; Immunohistochemistry

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Objective To study the effects of the expressions of endostatin, basic fibroblast growth factor (bFGF) and CD34 on oncogenesis and progression of gallbladder cancer, and to explore some valuable criterias for its biotherapy. Methods The expressions of endostatin, bFGF and CD34 were studied by means of immunohistochemistry (SP) in 61 cases of gallbladder cancer and 10 cases of normal cholecystic tissue, and microvessel density (MVD) was calculated by the expression of CD34. Their relationships with clinical pathological features were also investigated. Results The expression rates of endostatin in normal cholecystic tissue and in gallbladder cancer tissue were 40.00% (4/10) and 77.05% (47/61) respectively, which had statistical difference (P＜0.05). The expression of endostatin in 61 cases of caner was relational to clinical stage and metastasis of lymph nodes (P＜0.05), while no significant correlation was detected with sex and age of patient, location of tumor, size of tumor and histologic grade (P＞0.05). The expression rates of bFGF in normal cholecystic tissue and in gallbladder cancer tissue were 20.00%（2/10） and 67.21% （41/61） respectively, which had statistical difference (P＜0.05). The expression of bFGF in 61 cases of caner was relational to clinical stage and metastasis of lymph nodes (P＜0.05), while no significant correlation was detected with sex and age of patient, location of tumor, size of tumor and histologic grade (P＞0.05). MVD in gallbladder cancer tissue and in normal cholecystic tissue was （76.66±20.15） piece/HP and (29.53±5.03) piece/HP respectively, showing significant difference (P＜0.01). In 61 cases of cancer, MVD in clinical stage Ⅲ～Ⅴ 〔(80.53±17.98) piece/HP〕 was much higher than that in stage Ⅰ+Ⅱ 〔(46.79±5.38) piece/HP〕, P＜0.01; MVD was higher in those with lymph nodes metastasis 〔(94.60±7.28) piece/HP〕 than those without metastasis 〔(58.12±9.24) piece/HP〕, P＜0.01; and MVD was (60.59±14.71) piece/HP in histologic grade G1， (83.08±15.30) piece/HP in G2， and (96.53±6.92) piece/HP in G3, the difference was significant among them （P＜0.01）. There was no significant correlation between MVD and sex and age of patient, location of tumor and size of tumor (P＞0.05). There were statistically significant correlations between expressions of endostatin and MVD （P＜0.01）, expressions of bFGF and MVD （P＜0.01）. Conclusions The result suggests that endostatin, bFGF and CD34 play roles in oncogenesis and progression of gallbladder cancer. Detection of these proteins has positive effects on diagnosis, malignant degree determination and treatment of gallbladder cancer.

Citation： WEN Decai,GENG Jianli,GAO Ruohui,GUO Renxuan,ZHANG Yong. Study on Expressions and Significances of Endostatin, bFGF and CD34 in Gallbladder Cancer. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2009, 16(6): 470-474. doi: Copy

1.	] 邹声泉, 张林. 胆囊癌外科住院病例全国调查——3922例外科病理学诊断和治疗分析 [Ｊ]. 中华肝胆外科杂志, 1999; 5（6）： 363-365.
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6.	Weidner N. Intratumor microvessel density as a prognostic factor in cancer [Ｊ]. Am J Pathol, 1995; 147(1): 9-19.
7.	杨华，陈志新. 促血管生成素与胃癌血管生成的关系 [Ｊ]. 中国普外基础与临床杂志, 2007; 14(3): 368-370.
8.	何利丽, 冯一中, 顾永平. 血管相关因子在胰腺癌中的表达及意义 [Ｊ]. 中华消化杂志, 2005; 25(5): 300-301.
9.	Folkman J. Role of angiogenesis in tumor growth and metastasis [Ｊ]. Semin Oncol, 2002; 29(6 Suppl 16): 15-18.
10.	Folkman J. Antiangiogenesis in cancer therapy-endostatin and its mechanisms of action [Ｊ]. Exp Cell Res, 2006; 312(5): 594-607.
11.	Guenther U, Herbst H, Bauer M, et al. Collagen type ⅩⅧ/endostatin is differentially expressed in primary and metastatic colorectal cancers and ovarian carcinomas [Ｊ]. Br J Cancer, 2001; 85(10): 1540-1545.
12.	Strik HM, Schluesener HJ, Seid K, et al. Localization of endostatin in rat and human gliomas [Ｊ]. Cancer, 2001; 91(5): 1013-1019.
13.	Morimoto T, Aoyagi M, Tamaki M, et al. Increased levels of tissue endostatin in human malignant gliomas [Ｊ]. Clin Cancer Res, 2002; 8(9): 2933-2938.
14.	] Elagoz S, Egilmez R, Koyuncu A, et al. The intratumoral microvessel density and expression of bFGF and nm23-H1 in colorectal cancer [Ｊ]. Pathol Oncol Res, 2006; 12(1): 21-27.
15.	Hase T, Kawashiri S, Tanaka A, et al. Correlation of basic fibroblast growth factor expression with the invasion and the prognosis of oral squamous cell carcinoma [Ｊ]. J Oral Pathol Med, 2006; 35(3): 136-139.
16.	王天宝，李兆亭. 血管内皮生长因子及微血管密度与胃癌进展的关系 [Ｊ]. 中国普外基础与临床杂志, 2002; 9(3): 159-160.
17.	牛保华，吴萍，赵卫. 结直肠癌微血管密度与其病理特征的关系 [Ｊ]. 山西医科大学学报, 2007; 38(2): 124-125.
18.	Ikeguchi M, Cai J, Yamane N, et al. Clinical significance of spontaneous apoptosis in advanced gastric adenocarcinoma [Ｊ]. Cancer, 1999; 85(11): 2329-2335.
19.	张学利, 王铭河, 张华云, 等. 胆囊癌微血管密度的测定及其生物学意义 [Ｊ]. 中华肝胆外科杂志, 2005; 11（6）： 374.
20.	Giatromanolaki A, Sivridis E, Koukourakis MI, et al. Prognostic role of angiogenesis in operable carcinoma of the gallbladder [Ｊ]. Am J Clin Oncol, 2002; 25(1): 38-41.
21.	Abdollahi A, Hlatky L, Huber PE. Endostatin: the logic of antiangiogenic therapy [Ｊ]. Drug Resist Updat, 2005; 8(1-2): 59-74.

1. ] 邹声泉, 张林. 胆囊癌外科住院病例全国调查——3922例外科病理学诊断和治疗分析 [Ｊ]. 中华肝胆外科杂志, 1999; 5（6）： 363-365.
2. 郭仁宣, 许元鸿. 胆囊癌 [Ａ]. 见：郭仁宣主编. 胆道肿瘤外科学 [Ｍ]. 沈阳: 辽宁科学技术出版社, 2002: 240-257.
3. Folkman J. Angiogenesis in cancer, vascular, rheumatoid and other disease [Ｊ]. Nat Med, 1995; 1(1): 27-31.
4. 吕新生. 胆道肿瘤 [Ａ]. 见：吴在德主编. 外科学 [Ｍ]. 第5版. 北京: 人民卫生出版社, 2002: 632-634.
5. 许良中, 杨文涛. 免疫组织化学反应结果的判断标准 [Ｊ]. 中国癌症杂志, 1996; 6(4): 229-231.
6. Weidner N. Intratumor microvessel density as a prognostic factor in cancer [Ｊ]. Am J Pathol, 1995; 147(1): 9-19.
7. 杨华，陈志新. 促血管生成素与胃癌血管生成的关系 [Ｊ]. 中国普外基础与临床杂志, 2007; 14(3): 368-370.
8. 何利丽, 冯一中, 顾永平. 血管相关因子在胰腺癌中的表达及意义 [Ｊ]. 中华消化杂志, 2005; 25(5): 300-301.
9. Folkman J. Role of angiogenesis in tumor growth and metastasis [Ｊ]. Semin Oncol, 2002; 29(6 Suppl 16): 15-18.
10. Folkman J. Antiangiogenesis in cancer therapy-endostatin and its mechanisms of action [Ｊ]. Exp Cell Res, 2006; 312(5): 594-607.
11. Guenther U, Herbst H, Bauer M, et al. Collagen type ⅩⅧ/endostatin is differentially expressed in primary and metastatic colorectal cancers and ovarian carcinomas [Ｊ]. Br J Cancer, 2001; 85(10): 1540-1545.
12. Strik HM, Schluesener HJ, Seid K, et al. Localization of endostatin in rat and human gliomas [Ｊ]. Cancer, 2001; 91(5): 1013-1019.
13. Morimoto T, Aoyagi M, Tamaki M, et al. Increased levels of tissue endostatin in human malignant gliomas [Ｊ]. Clin Cancer Res, 2002; 8(9): 2933-2938.
14. ] Elagoz S, Egilmez R, Koyuncu A, et al. The intratumoral microvessel density and expression of bFGF and nm23-H1 in colorectal cancer [Ｊ]. Pathol Oncol Res, 2006; 12(1): 21-27.
15. Hase T, Kawashiri S, Tanaka A, et al. Correlation of basic fibroblast growth factor expression with the invasion and the prognosis of oral squamous cell carcinoma [Ｊ]. J Oral Pathol Med, 2006; 35(3): 136-139.
16. 王天宝，李兆亭. 血管内皮生长因子及微血管密度与胃癌进展的关系 [Ｊ]. 中国普外基础与临床杂志, 2002; 9(3): 159-160.
17. 牛保华，吴萍，赵卫. 结直肠癌微血管密度与其病理特征的关系 [Ｊ]. 山西医科大学学报, 2007; 38(2): 124-125.
18. Ikeguchi M, Cai J, Yamane N, et al. Clinical significance of spontaneous apoptosis in advanced gastric adenocarcinoma [Ｊ]. Cancer, 1999; 85(11): 2329-2335.
19. 张学利, 王铭河, 张华云, 等. 胆囊癌微血管密度的测定及其生物学意义 [Ｊ]. 中华肝胆外科杂志, 2005; 11（6）： 374.
20. Giatromanolaki A, Sivridis E, Koukourakis MI, et al. Prognostic role of angiogenesis in operable carcinoma of the gallbladder [Ｊ]. Am J Clin Oncol, 2002; 25(1): 38-41.
21. Abdollahi A, Hlatky L, Huber PE. Endostatin: the logic of antiangiogenic therapy [Ｊ]. Drug Resist Updat, 2005; 8(1-2): 59-74.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Study on Expressions and Significances of Endostatin, bFGF and CD34 in Gallbladder Cancer

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