Study on The Effect of Acute Suppurative Peritonitis Associated Ascitic Fluid on Experimental Liver Injury of Rats_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

FANG Xiang ¹ , CHEN Yuxiang ¹ , LIU Zhen ¹ , CHENG Ruochuan ² , ZHANG Yuetian ¹ , LI Jun ¹

1.Department of General Surgery, The People’s Hospital of Deyang City, Sichuan Province, Deyang 618000, China;;
2.Department of General Surgery, The First Affiliated Hospital of Kunming Medical College, Kunming 650032, China;

Corresponding author：

Keywords：

Acute suppurative peritonitis associated ascites fluid; Liver; Injury; Endotoxin; Tumor necrosis factor-α

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Objective To study the effect and intrinsic mechanism of acute suppurative peritonitis associated ascitic fluid (ASPAAF) on experimental liver injury of rats.
Methods Thirty-two male or female Sprague-Dawley (SD) rats were randomly divided into two groups: ASPAAF group (n=16) and control group (n=16), in which 8 ml ASPAAF or normal saline (NS) were injected into the peritoneal cavity, respectively. The rats were killed at each time intervals after peritoneal cavity injection (6 h and 12 h) respectively in two groups and specimens were made to detect the levels of serum TNF-α, endotoxin and liver function (AST, ALT and STB). The level of TNF-α in liver tissues was measured. The pathological change of liver was observed by microscope.
Results The levels of TNF-α, endotoxin, ALT, AST and STB in serum and the levels of TNF-α in liver tissues at different time points were markedly higher in ASPAAF group compared with those in control group (P＜0.05), and these indexes increased with increasing time in ASPAAF group (P＜0.05). In ASPAAF group, hepatic tissue appeared hydrops, even spotty necrosis and the changes at 6 h and 12 h were not obvious different. No abnormal pathological change of hepatic tissue was found in control group.
Conclusion ASPAAF can induce the injury of the liver in rats, which may involved in TNF-α and endotoxin.

Citation： FANG Xiang ,CHEN Yuxiang,LIU Zhen,CHENG Ruochuan,ZHANG Yuetian,LI Jun. Study on The Effect of Acute Suppurative Peritonitis Associated Ascitic Fluid on Experimental Liver Injury of Rats. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2009, 16(7): 555-559. doi: Copy

1.	姚咏明，盛志勇．我国创伤脓毒症基础研究新进展 [Ｊ]. 中华创伤杂志， 2003； 19(1): 912.
2.	Yang S, Koo DJ, Chaudry IH, et al. Glycine attenuates hepatocellular depression during early sepsis and reduces sepsisinduced mortality [Ｊ]. Crit Care Med, 2001; 29(6): 1201 1206.
3.	Erwin PJ, Lewis H, Dolan S, et al. Lipopolysaccharide binding protein in acute pancreatitis [Ｊ]. Crit Care Med, 2000; 28(1): 104109.
4.	Makhija R, Kingsnorth AN. Cytokine storm in acute pancreatitis [Ｊ]. J Hepatobiliary Pancreat Surg, 2002; 9(4): 401410.
5.	Davies MG, Hagen PO. Systemic inflammatory response syndrome [Ｊ]. Br J Surg, 1997; 84 (7): 920935.
6.	Liu D, Li C, Chen Y, et al. Nuclear import of proinflammatory transcription factors is required for massive liver apoptosis induced by bacterial lipopolysaccharide [Ｊ]. J Biol Chem, 2004; 279(46): 4843448442.
7.	Sass G, Heinlein S, Agli A, et al. Cytokine expression in three mouse models of experimental hepatitis [Ｊ]. Cytokine, 2002; 19(3): 115120.
8.	蒋力生，陈鹏. 脓毒症与内毒素血症的治疗现状与展望 [Ｊ]. 中国普外基础与临床杂志， 2004； 11(6): 551553.
9.	宋文哲，宋燕，谭岩，等. 氨基胍对内毒素休克大鼠肝损伤的保护作用研究 [Ｊ]. 中国普外基础与临床杂志， 2003； 10(4): 347350.
10.	Thirunavukkarasu C, Uemura T, Wang LF, et al. Normal rat hepatic stellate cells respond to endotoxin in LBPindependent manner to produce inhibitor(s) of DNA synthesis in hepatocytes [Ｊ]. J Cell Physiol, 2005; 204(4): 654665.[11]Schwabe RF, Brenner DA. Mechanisms of liver injury. I. TNF induced liver injury: role of IKK, JNK, and ROS pathways [Ｊ]. Am J Physiol Gastrointest Liver Physiol, 2006; 290(4): G583G589.
11.	Skinner NA, MacIsaac CM, Hamilton JA, et al. Regulation of Tolllike receptor (TLR) 2 and TLR4 on CD14dimCD16+ monocytes in response to sepsisrelated antigens [Ｊ]. Clin Exp Immunol, 2005; 141(2): 270278.
12.	Kobayashi Y, Mori M, Naruto T, et al. Dynamic movement of cytochrome c from mitochondria into cytosol and peripheral circulation in massive hepatic cell injury [Ｊ]. Pediatr Int 2004; 46(6): 685692.
13.	石力，田伏洲，李旭，等. 急性胰腺炎大鼠肝脏NFκB对TNFα表达的调控及其在肝损伤中的作用 [Ｊ]. 中国普外基础与临床杂志， 2004； 11(3): 228230.
14.	GómezCambronero LG, Sabater L, Pereda J, et al. Role of cytokines and oxidative stress in the pathophysiology of acute pancreatitis: therapeutical implications [Ｊ]. Curr Drug Targets Inflamm Allergy, 2002; 1(4): 393403.
15.	Pastor CM, Frossard JL. Are genetically modified mice useful for the understanding of acute pancreatitis? [Ｊ]. FASEB J, 2001; 15(6): 893897.
16.	Leindler L, Morschl E, László F, et al. Importance of cytokines, nitric oxide, and apoptosis in the pathological process of necrotizing pancreatitis in rats [Ｊ]. Pancreas, 2004; 29(2): 157161.
17.	Vliagoftis H, Befus AD, Hollenberg MD, et al. Airway epithelial cells release eosinophil survivalpromoting factors (GMCSF) after stimulation of proteinaseactivated receptor 2 [Ｊ]. J Allergy Clin Immunol, 2001； 107(4): 679685.
18.	Van Pelt LJ, Huisman MV, Weening RS, et al. A single dose of granulocytemacrophage colony stimulating factor induces systemic interleukin8 release and neutrophil activation in healthy volunteers [Ｊ]. Blood, 1996; 87(12): 53055313.
19.	Jean LF, Ashok KS, Nicolas M, et al. In vivo evidence for the role of GMCSF as a mediator in acute pancreatitisassociated lung injury [Ｊ]. Am J Physiol Lung Cell Mol Physiol, 2002; 283(3): 541548.
20.	O’Neill S, O’Neill AJ, Conroy E, et al. Altered caspase expression results in delayed neutrophil apoptosis in acute pancreatitis [Ｊ]. J Leukoc Biol, 2000; 68(1): 1520.
21.	Botha AJ, Moore FA, Moore EE, et al. Early neutrophil sequestration after injury: a pathogenic mechanism for multiple organ failure [Ｊ]. J Trauma, 1995; 39(3): 411417.
22.	刘震，程若川，方向，等. PAAF与ASPAAF诱导的肠损伤比较的实验研究 [Ｊ]. 中国普外基础与临床杂志， 2008； 15(8): 555559.
23.	陈哲宇，齐清会，严律南. 细菌性腹膜炎诱导大鼠多器官功能不全综合征后结肠运动的变化 [Ｊ]. 中国普外基础与临床杂志， 2006； 13(3): 287290.
24.	肖菲，康焰. 肠道细菌移位的研究现状 [Ｊ]. 中国普外基础与临床杂志， 2006； 13(2): 240243.

1. 姚咏明，盛志勇．我国创伤脓毒症基础研究新进展 [Ｊ]. 中华创伤杂志， 2003； 19(1): 912.
2. Yang S, Koo DJ, Chaudry IH, et al. Glycine attenuates hepatocellular depression during early sepsis and reduces sepsisinduced mortality [Ｊ]. Crit Care Med, 2001; 29(6): 1201 1206.
3. Erwin PJ, Lewis H, Dolan S, et al. Lipopolysaccharide binding protein in acute pancreatitis [Ｊ]. Crit Care Med, 2000; 28(1): 104109.
4. Makhija R, Kingsnorth AN. Cytokine storm in acute pancreatitis [Ｊ]. J Hepatobiliary Pancreat Surg, 2002; 9(4): 401410.
5. Davies MG, Hagen PO. Systemic inflammatory response syndrome [Ｊ]. Br J Surg, 1997; 84 (7): 920935.
6. Liu D, Li C, Chen Y, et al. Nuclear import of proinflammatory transcription factors is required for massive liver apoptosis induced by bacterial lipopolysaccharide [Ｊ]. J Biol Chem, 2004; 279(46): 4843448442.
7. Sass G, Heinlein S, Agli A, et al. Cytokine expression in three mouse models of experimental hepatitis [Ｊ]. Cytokine, 2002; 19(3): 115120.
8. 蒋力生，陈鹏. 脓毒症与内毒素血症的治疗现状与展望 [Ｊ]. 中国普外基础与临床杂志， 2004； 11(6): 551553.
9. 宋文哲，宋燕，谭岩，等. 氨基胍对内毒素休克大鼠肝损伤的保护作用研究 [Ｊ]. 中国普外基础与临床杂志， 2003； 10(4): 347350.
10. Thirunavukkarasu C, Uemura T, Wang LF, et al. Normal rat hepatic stellate cells respond to endotoxin in LBPindependent manner to produce inhibitor(s) of DNA synthesis in hepatocytes [Ｊ]. J Cell Physiol, 2005; 204(4): 654665.[11]Schwabe RF, Brenner DA. Mechanisms of liver injury. I. TNF induced liver injury: role of IKK, JNK, and ROS pathways [Ｊ]. Am J Physiol Gastrointest Liver Physiol, 2006; 290(4): G583G589.
11. Skinner NA, MacIsaac CM, Hamilton JA, et al. Regulation of Tolllike receptor (TLR) 2 and TLR4 on CD14dimCD16+ monocytes in response to sepsisrelated antigens [Ｊ]. Clin Exp Immunol, 2005; 141(2): 270278.
12. Kobayashi Y, Mori M, Naruto T, et al. Dynamic movement of cytochrome c from mitochondria into cytosol and peripheral circulation in massive hepatic cell injury [Ｊ]. Pediatr Int 2004; 46(6): 685692.
13. 石力，田伏洲，李旭，等. 急性胰腺炎大鼠肝脏NFκB对TNFα表达的调控及其在肝损伤中的作用 [Ｊ]. 中国普外基础与临床杂志， 2004； 11(3): 228230.
14. GómezCambronero LG, Sabater L, Pereda J, et al. Role of cytokines and oxidative stress in the pathophysiology of acute pancreatitis: therapeutical implications [Ｊ]. Curr Drug Targets Inflamm Allergy, 2002; 1(4): 393403.
15. Pastor CM, Frossard JL. Are genetically modified mice useful for the understanding of acute pancreatitis? [Ｊ]. FASEB J, 2001; 15(6): 893897.
16. Leindler L, Morschl E, László F, et al. Importance of cytokines, nitric oxide, and apoptosis in the pathological process of necrotizing pancreatitis in rats [Ｊ]. Pancreas, 2004; 29(2): 157161.
17. Vliagoftis H, Befus AD, Hollenberg MD, et al. Airway epithelial cells release eosinophil survivalpromoting factors (GMCSF) after stimulation of proteinaseactivated receptor 2 [Ｊ]. J Allergy Clin Immunol, 2001； 107(4): 679685.
18. Van Pelt LJ, Huisman MV, Weening RS, et al. A single dose of granulocytemacrophage colony stimulating factor induces systemic interleukin8 release and neutrophil activation in healthy volunteers [Ｊ]. Blood, 1996; 87(12): 53055313.
19. Jean LF, Ashok KS, Nicolas M, et al. In vivo evidence for the role of GMCSF as a mediator in acute pancreatitisassociated lung injury [Ｊ]. Am J Physiol Lung Cell Mol Physiol, 2002; 283(3): 541548.
20. O’Neill S, O’Neill AJ, Conroy E, et al. Altered caspase expression results in delayed neutrophil apoptosis in acute pancreatitis [Ｊ]. J Leukoc Biol, 2000; 68(1): 1520.
21. Botha AJ, Moore FA, Moore EE, et al. Early neutrophil sequestration after injury: a pathogenic mechanism for multiple organ failure [Ｊ]. J Trauma, 1995; 39(3): 411417.
22. 刘震，程若川，方向，等. PAAF与ASPAAF诱导的肠损伤比较的实验研究 [Ｊ]. 中国普外基础与临床杂志， 2008； 15(8): 555559.
23. 陈哲宇，齐清会，严律南. 细菌性腹膜炎诱导大鼠多器官功能不全综合征后结肠运动的变化 [Ｊ]. 中国普外基础与临床杂志， 2006； 13(3): 287290.
24. 肖菲，康焰. 肠道细菌移位的研究现状 [Ｊ]. 中国普外基础与临床杂志， 2006； 13(2): 240243.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Study on The Effect of Acute Suppurative Peritonitis Associated Ascitic Fluid on Experimental Liver Injury of Rats

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