Study on Gradual Oral Diethylnitrosamine Induced Cirrhotic Model in Rats under Avoirdupois Monitoring_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

HUA Yunpeng ¹ , LI Shaoqiang ¹ , LAI Jiaming ¹ , PENG Baogang ¹ , LIANG Huizhen ² , LIANG Lijian ¹

1.Department of Hepatobiliary Surgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China;;
2.Department of Pathology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China;

Corresponding author：

Keywords：

Liver cirrhosis; Animal model; Diethylnitrosamine

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Objective To study the effect and feasibility of gradual oral diethylnitrosamine (DENA) induced liver cirrhotic model in rats under avoirdupois monitoring.
Methods Fifty Wistar rats (6 weeks old) were divided into 3 groups: normal control group （n=10）, traditional DENA induction group (receiving traditional oral DENA treatment, n=20）, gradual DENA induction group (receiving gradual oral DENA treatment under avoirdupois monitoring, n=20). The weight, mortality and liver cirrhosis formation were observed.
Results After 4 weeks of inducing cirrhosis, the weight of traditional DENA induction group 〔(234.9±27.1) g〕 was significantly lower than that of normal control group 〔(264.8±33.7) g, P＜0.05〕. After 8 weeks of inducing cirrhosis, the weight of traditional DENA induction group 〔(251.5±34.3) g〕 was significantly lower than that of normal control group 〔(303.2±49.4) g，　P＜0.01〕　and gradual DENA induction group 〔(277.5±27.6) g， P＜0.05〕. However, the difference between normal control group and gradual DENA induction group was not remarkable (P＞0.05). The mortality in traditional DENA induction group (35%) was significantly higher than that in normal control group (0) and gradual DENA induction group (0)， P＜0.05. But the rate of cirrhosis formation both in traditional DENA induction group and gradual DENA induction group was 100%.
Conclusion Oral DENA induced cirrhotic model in rats is a simple, reproducible and reliable technique. Gradual oral technique, in which DENA is given under avoirdupois monitoring, can improve rat’s security and reduce mortality.

Citation： HUA Yunpeng ,LI Shaoqiang,LAI Jiaming,PENG Baogang,LIANG Huizhen,LIANG Lijian. Study on Gradual Oral Diethylnitrosamine Induced Cirrhotic Model in Rats under Avoirdupois Monitoring. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2009, 16(8): 625-627. doi: Copy

1.	姜洪池. 肝硬变门静脉高压症外科治疗的今日思考 [Ｊ]. 中国普外基础与临床杂志， 2008； 15（12）： 871-872.
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14.	Bishayee A, Dhir N. Resveratrol-mediated chemoprevention of diethylnitrosamine-initiated hepatocarcinogenesis: inhibition of cell proliferation and induction of apoptosis [Ｊ]. Chem Biol Interact, 2009; 179(2-3): 131-144.
15.	Teoh NC, Dan YY, Swisshelm K, et al. Defective DNA str-and break repair causes chromosomal instability and accelerates liver carcinogenesis in mice [Ｊ]. Hepatology, 2008; 47(6): 2078-2088.
16.	华赟鹏, 马毅, 李绍强, 等. 奥曲肽对二乙基亚硝胺诱导的大鼠肝癌的影响 [Ｊ]. 中华普通外科杂志, 2008; 23(12): 924-927.
17.	马毅, 华赟鹏, 陈祖兵, 等. 大鼠肝癌模型的化学诱导及肝癌肝移植模型的建立 [Ｊ]. 中华医学杂志, 2006; 86(12): 850-853.
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19.	Chae HB, Jang LC, Park SM, et al. An experimental model of hepatic fibrosis induced by alcohol and CCl4: can the lipopolysaccharide prevent liver injury induced by alcohol and CCl4 ? [Ｊ]. Taehan Kan Hakhoe Chi, 2002; 8(2): 173-178.

1. 姜洪池. 肝硬变门静脉高压症外科治疗的今日思考 [Ｊ]. 中国普外基础与临床杂志， 2008； 15（12）： 871-872.
2. Cichoz-Lach H, Celiński K, Somka M, et al. Pathophysiology of portal hypertension [Ｊ]. J Physiol Pharmacol, 2008; 59 (Suppl 2): 231-238.
3. Pinzani M, Vizzutti F. Fibrosis and cirrhosis reversibility: clinical features and implications [Ｊ]. Clin Liver Dis, 2008; 12(4): 901-913.
4. Laleman W, Nevens F. Cirrhotic portal hypertension: current and future medical therapy for primary and secondary prevention of variceal bleeding [Ｊ]. Minerva Med, 2006; 97(4): 325-345.
5. Singh NA, Reau N. Management of hepatitis B virus [Ｊ]. J Antimicrob Chemother, 2008; 62(2): 224-228.
6. 张贵阳，杨卫平，陈皓. 肝硬化动物模型构建的研究进展 [Ｊ]. 外科理论与实践, 2008; 13(3): 266-269.
7. 郑敏, 陈聪, 赵雅萍, 等. 探讨大鼠肝硬化动物模型的建立方法 [Ｊ]. 肝胆外科杂志, 2006; 12(2): 136-137.
8. 周丁华，应大君. 大鼠肝硬变门脉高压症模型建立方法改进 [Ｊ]. 中国普外基础与临床杂志， 2000； 7（1）： 22-24.
9. Vanheule E, Geerts AM, Van Huysse J， et al. An intravital microscopic study of the hepatic microcirculation in cirrhotic mice models: relationship between fibrosis and angiogenesis [Ｊ]. Int J Exp Pathol, 2008; 89(6): 419-432.
10. Jaramillo-Juárez F, Rodríguez-Vázquez ML, Rincón-Sánchez AR, et al. Acute renal failure induced by carbon tetrachloride in rats with hepatic cirrhosis [Ｊ]. Ann Hepatol, 2008; 7(4): 331-338.
11. Zhang JJ, Meng XK, Dong C, et al. Development of a new animal model of liver cirrhosis in swine [Ｊ]. Eur Surg Res, 2009; 42(1): 35-39.
12. Zhang M, Gong Y, Corbin I, et al. The effects of light and moderate-heavy ethanol exposure on the development of cirrhosis in rats [Ｊ]. Dig Dis Sci, 2006; 51(10): 1732-1737.
13. Qi Y, Chen X, Chan CY, et al. Two-dimensional differential gel electrophoresis/analysis of diethylnitrosamine induced rat hepatocellular carcinoma [Ｊ]. Int J Cancer, 2008; 122(12): 2682-2688.
14. Bishayee A, Dhir N. Resveratrol-mediated chemoprevention of diethylnitrosamine-initiated hepatocarcinogenesis: inhibition of cell proliferation and induction of apoptosis [Ｊ]. Chem Biol Interact, 2009; 179(2-3): 131-144.
15. Teoh NC, Dan YY, Swisshelm K, et al. Defective DNA str-and break repair causes chromosomal instability and accelerates liver carcinogenesis in mice [Ｊ]. Hepatology, 2008; 47(6): 2078-2088.
16. 华赟鹏, 马毅, 李绍强, 等. 奥曲肽对二乙基亚硝胺诱导的大鼠肝癌的影响 [Ｊ]. 中华普通外科杂志, 2008; 23(12): 924-927.
17. 马毅, 华赟鹏, 陈祖兵, 等. 大鼠肝癌模型的化学诱导及肝癌肝移植模型的建立 [Ｊ]. 中华医学杂志, 2006; 86(12): 850-853.
18. Mullen KD, McCullough AJ. Problems with animal models of chronic liver disease: suggestions for improvement in standardization [Ｊ]. Hepatology, 1989; 9(3): 500-503.
19. Chae HB, Jang LC, Park SM, et al. An experimental model of hepatic fibrosis induced by alcohol and CCl4: can the lipopolysaccharide prevent liver injury induced by alcohol and CCl4 ? [Ｊ]. Taehan Kan Hakhoe Chi, 2002; 8(2): 173-178.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Study on Gradual Oral Diethylnitrosamine Induced Cirrhotic Model in Rats under Avoirdupois Monitoring

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