Investigation of Insulin-Like Growth Factor-1 Expression in Colorectal Cancer_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

HUANG Guilin ¹ , WU Yiping ¹ , LEI Na ²

1.Department of Plastic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China;;
2.The Second Department of General Surgery, The First Affiliated Hospital of Shihezi University, Shihezi 832002, Xinjiang, ChinaCorresponding Author: HUANG Gui-lin, E-mail: hglzh2005@sina.com;

Corresponding author：

Keywords：

Insulin-like growth factor 1; Liver-specific insulin-like growth factor 1 deficient mice; Colorectal cancer; Immunohistochemical assay

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Objective 　To establish a stable colorectal cancer model in liver specific insulin-like growth factor (IGF)-1 deficient (LID) mice and examine the potential relationship between IGF-1 level and risk of mice constitutional colorectal cancer.
Methods 　①Establishment of a colorectal cancer model: The LID mice, in which IGF-1 level in circulation was 25% of BALB/c mice. Induction of colorectal cancer was achieved by using the 1,1 Dimethylhydrazine (DMH) with hypodermic injection at transverse part. ②Eighty fresh samples of cancer tissues and adjacent tissues were obtained from LID mice (experimental group) and BALB/c mice (control group). The expression of IGF-1 was studied by immunohistochemical assay (SP method).
Results 　①Weight loss occurred in both experimental group and control group after injection. Compared with the body weight before injection on 18 weeks and 24 weeks in each group, there were significant differences after injection at the same phase in each group (P＜0.05). ②The results of IGF-1 expression in cancer tissues and adjacent tissues: IGF-1 got a diffuse distribution in cancer cell cytoplasm. The positive expressions of IGF-1 in the cancer tissues and their adjacent cancer tissues were 6/7, 2/7 and 13/16, 7/16 respectively in experimental group and control group. There were significant differences between the cancer tissues and adjacent tissues inside both groups (P＜0.05). There were no significant differences inside both of cancer tissues and adjacent tissues respectively between experimental group and control group (P gt;0.05).
Conclusion 　In the established colorectal cancer model by DMH, IGF-1 plays an important role in the development and progression of colorectal cancer.

Citation： HUANG Guilin,WU Yiping,LEI Na. Investigation of Insulin-Like Growth Factor-1 Expression in Colorectal Cancer. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2009, 16(9): 693-697. doi: Copy

1.	Ma J, Pollak MN, Giovannucci E, et al. Prospective study of colorectal cancer risk in men and plasma levels of insulin-like growth factor (IGF)-Ⅰ and IGF-binding protein-3 [Ｊ]. J Natl Cancer Inst, 1999; 91(7): 620-625.
2.	张珊, 陈国庆, 唐鸿波, 等. 血清胰岛素样生长因子-1对癌性恶病质小鼠营养状况的影响 [Ｊ]. 中国普外基础与临床杂志, 2008; 15(2): 112-115.
3.	Wolpin BM, Michaud DS, Giovannucci EL, et al. Circulating insulin-like growth factor binding protein-1 and the risk of pancreatic cancer [Ｊ]. Cancer Res, 2007; 67(16): 7923-7928.
4.	任玉萍, 叶子荣, 唐泓波, 等. 利用IGF-1基因敲除鼠乳腺癌模型研究IGF-1对血管生成的影响 [Ｊ]. 中国普外基础与临床杂志, 2008; 15(11): 820-824.
5.	Sridhar SS, Goodwin PJ. Insulin-insulin-like growth factor axis and colon cancer [Ｊ]. J Clin Oncol, 2009; 27(2): 165-167.
6.	牛坚, 李向农, 韩泽广. 运用siRNA建立稳定低表达IGF1R基因的肝癌细胞株的实验研究 [Ｊ]. 中国普外基础与临床杂志, 2007; 14(6): 679-684.
7.	Hankinson SE, Willett WC, Colditz GA, et al. Circulating concentrations of insulin-like growth factor-Ⅰ and risk of breast cancer [Ｊ]. Lancet, 1998; 351(9113): 1393-1396.
8.	Chan JM, Stampfer MJ, Giovannucci E, et al. Plasma insulin-like growth factor-Ⅰ and prostate cancer risk: a prospective study [Ｊ]. Science, 1998; 279(5350): 563-566.
9.	Yu H, Spitz MR, Mistry J, et al. Plasma levels of insulin-like growth factor-Ⅰ and lung cancer risk: a case-control analysis [Ｊ]. J Natl Cancer Inst, 1999; 91(2): 151-156.
10.	Yakar S, Liu JL, Stannard B, et al. Normal growth and development in the absence of hepatic insulin-like growth factor Ⅰ [Ｊ]. Proc Natl Acad Sci USA, 1999; 96(13): 7324-7329.
11.	李玉林主编. 病理学 [M]. 第6版. 北京: 人民卫生出版社, 2004: 95-111.
12.	郭宝文，吴敏兰. IGF-Ⅱ、IGF-ⅠR 在大肠癌中的表达 [Ｊ]. 山东医药, 2005; 45(10): 6-8.
13.	Takahari D, Yamada Y, Okita NT, et al. Relationships of insulin-like growth factor-1 receptor and epidermal growth factor receptor expression to clinical outcomes in patients with colorectal cancer [Ｊ]. Oncology, 2009; 76(1): 42-48.
14.	Wu Y, Yakar S, Zhao L, et al. Circulating insulin-like gro-wth factor-Ⅰ levels regulate colon cancer growth and metastasis [Ｊ]. Cancer Res, 2002; 62(4): 1030-1035.
15.	杨琳，郭宝文，赵魁. IGF-Ⅰ、IGF-Ⅱ 在大肠癌中的表达及临床意义 [Ｊ]. 山东医药, 2005; 45(9): 35-36.
16.	吴毅平, Cui C, Miyoshi K, 等. 肝特异性胰岛素样生长因子Ⅰ基因缺失鼠原发性乳腺癌模型的建立 [Ｊ]. 华中科技大学学报(医学版), 2004; 33(2): 180-183.
17.	Durai R, Yang W, Gupta S, et al. The role of the insulin-like growth factor system in colorectal cancer: review of current knowledge [Ｊ]. Int J Colorectal Dis, 2005; 20(3): 203-220.
18.	Hakam A, Yeatman TJ, Lu L, et al. Expression of insulin-like growth factor-1 receptor in human colorectal cancer [Ｊ]. Hum Pathol, 1999; 30(10): 1128-1133.
19.	DeLellis K, Rinaldi S, Kaaks RJ, et al. Dietary and lifestyle correlates of plasma insulin-like growth factor-Ⅰ (IGF-Ⅰ) and IGF binding protein-3 (IGFBP-3): the multiethnic cohort [Ｊ]. Cancer Epidemiol Biomarkers Prev, 2004; 13(9): 1444-1451.
20.	Jenkins PJ, Bustin SA. Evidence for a link between IGF-Ⅰ and cancer [Ｊ]. Eur J Endocrinol, 2004; 151 Suppl 1: S17-S22.

1. Ma J, Pollak MN, Giovannucci E, et al. Prospective study of colorectal cancer risk in men and plasma levels of insulin-like growth factor (IGF)-Ⅰ and IGF-binding protein-3 [Ｊ]. J Natl Cancer Inst, 1999; 91(7): 620-625.
2. 张珊, 陈国庆, 唐鸿波, 等. 血清胰岛素样生长因子-1对癌性恶病质小鼠营养状况的影响 [Ｊ]. 中国普外基础与临床杂志, 2008; 15(2): 112-115.
3. Wolpin BM, Michaud DS, Giovannucci EL, et al. Circulating insulin-like growth factor binding protein-1 and the risk of pancreatic cancer [Ｊ]. Cancer Res, 2007; 67(16): 7923-7928.
4. 任玉萍, 叶子荣, 唐泓波, 等. 利用IGF-1基因敲除鼠乳腺癌模型研究IGF-1对血管生成的影响 [Ｊ]. 中国普外基础与临床杂志, 2008; 15(11): 820-824.
5. Sridhar SS, Goodwin PJ. Insulin-insulin-like growth factor axis and colon cancer [Ｊ]. J Clin Oncol, 2009; 27(2): 165-167.
6. 牛坚, 李向农, 韩泽广. 运用siRNA建立稳定低表达IGF1R基因的肝癌细胞株的实验研究 [Ｊ]. 中国普外基础与临床杂志, 2007; 14(6): 679-684.
7. Hankinson SE, Willett WC, Colditz GA, et al. Circulating concentrations of insulin-like growth factor-Ⅰ and risk of breast cancer [Ｊ]. Lancet, 1998; 351(9113): 1393-1396.
8. Chan JM, Stampfer MJ, Giovannucci E, et al. Plasma insulin-like growth factor-Ⅰ and prostate cancer risk: a prospective study [Ｊ]. Science, 1998; 279(5350): 563-566.
9. Yu H, Spitz MR, Mistry J, et al. Plasma levels of insulin-like growth factor-Ⅰ and lung cancer risk: a case-control analysis [Ｊ]. J Natl Cancer Inst, 1999; 91(2): 151-156.
10. Yakar S, Liu JL, Stannard B, et al. Normal growth and development in the absence of hepatic insulin-like growth factor Ⅰ [Ｊ]. Proc Natl Acad Sci USA, 1999; 96(13): 7324-7329.
11. 李玉林主编. 病理学 [M]. 第6版. 北京: 人民卫生出版社, 2004: 95-111.
12. 郭宝文，吴敏兰. IGF-Ⅱ、IGF-ⅠR 在大肠癌中的表达 [Ｊ]. 山东医药, 2005; 45(10): 6-8.
13. Takahari D, Yamada Y, Okita NT, et al. Relationships of insulin-like growth factor-1 receptor and epidermal growth factor receptor expression to clinical outcomes in patients with colorectal cancer [Ｊ]. Oncology, 2009; 76(1): 42-48.
14. Wu Y, Yakar S, Zhao L, et al. Circulating insulin-like gro-wth factor-Ⅰ levels regulate colon cancer growth and metastasis [Ｊ]. Cancer Res, 2002; 62(4): 1030-1035.
15. 杨琳，郭宝文，赵魁. IGF-Ⅰ、IGF-Ⅱ 在大肠癌中的表达及临床意义 [Ｊ]. 山东医药, 2005; 45(9): 35-36.
16. 吴毅平, Cui C, Miyoshi K, 等. 肝特异性胰岛素样生长因子Ⅰ基因缺失鼠原发性乳腺癌模型的建立 [Ｊ]. 华中科技大学学报(医学版), 2004; 33(2): 180-183.
17. Durai R, Yang W, Gupta S, et al. The role of the insulin-like growth factor system in colorectal cancer: review of current knowledge [Ｊ]. Int J Colorectal Dis, 2005; 20(3): 203-220.
18. Hakam A, Yeatman TJ, Lu L, et al. Expression of insulin-like growth factor-1 receptor in human colorectal cancer [Ｊ]. Hum Pathol, 1999; 30(10): 1128-1133.
19. DeLellis K, Rinaldi S, Kaaks RJ, et al. Dietary and lifestyle correlates of plasma insulin-like growth factor-Ⅰ (IGF-Ⅰ) and IGF binding protein-3 (IGFBP-3): the multiethnic cohort [Ｊ]. Cancer Epidemiol Biomarkers Prev, 2004; 13(9): 1444-1451.
20. Jenkins PJ, Bustin SA. Evidence for a link between IGF-Ⅰ and cancer [Ｊ]. Eur J Endocrinol, 2004; 151 Suppl 1: S17-S22.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Investigation of Insulin-Like Growth Factor-1 Expression in Colorectal Cancer

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