Expression and Significance of A Novel Gene BC047440 in Multiplicate Malignant Tumor Tissues_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

ZHENG Lu , LI Jing , LIANG Ping , YANG Tonghan , HUANG Xiaobing , LIU Shicheng

Department of Hepatobiliary Surgery, Xinqiao Hospital, The Third Military Medical University, Chongqing 400037, China;

Corresponding author：

Keywords：

Carcinoma,BC047440 gene,Reverse transcription-polymerase chain reaction,Semi-quantitative

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【 Abstract 】 Objective To study the mRNA expression of BC047440 gene in multiplicate malignant tumor tissues and the corresponding adjacent tissues, and to investigate its roles in the carcinogenesis and development of malignant tumors. Methods Forty-eight cases of malignant tumor tissues and their adjacent non-cancerous tissues were examined. The mRNA expression of BC047440 gene in those tissues of liver cancer, cholangiocarcinoma, gastric cancer, carcinoma of large intestine, glioma, and breast cancer were measured by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). Results ① The mRNA expressions of BC047440 gene in liver cancer, gastric cancer, cholangiocarcinoma and carcinoma of large intestine were significantly higher than those in their adjacent non-cancerous tissues (P lt;0.05 or 0.01). BC047440 gene were highly expressed in both glioma and its adjacent tissues (P gt;0.05), and poorly expressed in both breast cancer and its adjacent tissues (P gt;0.05). ② There were close relationships between BC047440 gene expression and clinicopathologic findings of liver cancer, including tumor size and portal vein invasion (P lt;0.05). ③ There were also close relationships between BC047440 gene expression and different clinical stages in alimentary canal cancers (P lt;0.05). Conclusion The over expression of BC047440 gene may be related with the growth, infiltration and metastasis of some malignant tumors, including liver cancer, cholangiocarcinoma, gastric cancer, carcinoma of large intestine and glioma.

Citation： ZHENG Lu,LI Jing,LIANG Ping,YANG Tonghan,HUANG Xiaobing,LIU Shicheng. Expression and Significance of A Novel Gene BC047440 in Multiplicate Malignant Tumor Tissues. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2008, 15(3): 185-189. doi: Copy

1.	20 Vasko VV, Saji M. Molecular mechanisms involved in differentiated thyroid cancer invasion and metastasis [Ｊ] . Curr Opin Oncol, 2007; 19(1) ∶ 11.
2.	李靖，韩本立，黄桂君，等. 肝癌组织差异表达基因cDNA序列的筛选与鉴定 [Ｊ]. 中华医学遗传学杂志, 2003; 20(1)∶49.
3.	赵弘智, 李靖, 梁平, 等. 一条肝癌相关新基因的全长cDNA克隆 [Ｊ]. 中华肝脏病杂志, 2005; 13(7)∶483.
4.	Technical manual & SV total RNA isolation system. Available from: URL: http://www. promega.com/tbs/tm048/tm048.html.
5.	Marchisone C, Pfeffer U, Del Grosso F, et al. Progress towards gene therapy for cancer [Ｊ]. J Exp Clin Cancer Res, 2000; 19(3)∶261.
6.	Ohta K, Ohigashi M, Naganawa A, et al. Histone acetyltransferase MOZ acts as a co-activator of Nrf2-MafK and induces tumour marker gene expression during hepatocarcinogenesis [Ｊ]. Biochem J, 2007; 402(3)∶559.
7.	Hernández-Alcoceba R, Sangro B, Prieto J. Gene therapy of liver cancer [Ｊ]. Ann Hepatol, 2007; 6(1)∶5.
8.	Osada S, Naganawa A, Misonou M, et al. Altered gene expression of transcriptional regulatory factors in tumor marker-positive cells during chemically induced hepatocarcinogenesis [Ｊ]. Toxicol Lett, 2006; 167(2)∶106.
9.	Yeh SH, Chiu CM, Chen CL, et al. Somatic mutations at the trinucleotide repeats of androgen receptor gene in male hepatocellular carcinoma [Ｊ]. Int J Cancer, 2007; 120(8)∶1610.
10.	王万祥, 欧阳晓辉, 孟兴凯, 等. 肝细胞癌组织中CT9基因mRNA表达的初步研究 [Ｊ]. 中国普外基础与临床杂志, 2006; 13(2)∶170.
11.	李新明，陶凯雄, 王国斌, 等. 黑色素瘤抗原基因-3在直肠癌的表达及意义 [Ｊ]. 中国普外基础与临床杂志, 2006; 13(3)∶308.
12.	Bignotti E, Tassi RA, Calza S, et al. Differential gene expression profiles between tumor biopsies and short-term primary cultures of ovarian serous carcinomas: identification of novel molecular biomarkers for early diagnosis and therapy [Ｊ]. Gynecol Oncol, 2006; 103(2)∶405.
13.	Giri D. Gene microarrays in tumor diagnosis: opportunities and challenges [Ｊ]. Med Health R I, 2005; 88(7)∶212.
14.	Hatakeyama H, Akita H, Kogure K, et al. Development of a novel systemic gene delivery system for cancer therapy with a tumor-specific cleavable PEG-lipid [Ｊ]. Gene Ther, 2007; 14(1)∶68.
15.	Bazan-Peregrino M, Seymour LW, Harris AL. Gene therapy targeting to tumor endothelium [Ｊ]. Cancer Gene Ther, 2007; 14(2)∶117.
16.	Pastorakova A, Hlubinova K, Jakubikova J, et al. Combine cancer gene therapy harnessing plasmids expressing human tumor necrosis factor alpha and Herpes simplex thymidine kinase suicide gene [Ｊ]. Neoplasma, 2006; 53(5)∶353.
17.	Ternovoi VV, Curiel DT, Smith BF, et al. Adenovirus-mediated p53 tumor suppressor gene therapy of osteosarcoma [Ｊ]. Lab Invest, 2006; 86(8)∶748.
18.	周建波, 李靖, 梁平, 等. 肝细胞癌高表达新基因BC047440功能的研究 [Ｊ]. 中华实验外科杂志, 2006; 23（3）∶288.
19.	赵弘智, 梁平, 李靖, 等. 肝癌相关cDNA片段的快速克隆和表达 [Ｊ]. 世界华人消化杂志, 2004; 12（8）∶1785.
20.	Strausberg RL, Feingold EA, Grouse LH, et al. Generation and initial analysis of more than 15 000 full-length human and mouse cDNA sequences [Ｊ]. Proc Natl Acad Sci USA, 2002; 99(26)∶16899.

1. 20 Vasko VV, Saji M. Molecular mechanisms involved in differentiated thyroid cancer invasion and metastasis [Ｊ] . Curr Opin Oncol, 2007; 19(1) ∶ 11.
2. 李靖，韩本立，黄桂君，等. 肝癌组织差异表达基因cDNA序列的筛选与鉴定 [Ｊ]. 中华医学遗传学杂志, 2003; 20(1)∶49.
3. 赵弘智, 李靖, 梁平, 等. 一条肝癌相关新基因的全长cDNA克隆 [Ｊ]. 中华肝脏病杂志, 2005; 13(7)∶483.
4. Technical manual & SV total RNA isolation system. Available from: URL: http://www. promega.com/tbs/tm048/tm048.html.
5. Marchisone C, Pfeffer U, Del Grosso F, et al. Progress towards gene therapy for cancer [Ｊ]. J Exp Clin Cancer Res, 2000; 19(3)∶261.
6. Ohta K, Ohigashi M, Naganawa A, et al. Histone acetyltransferase MOZ acts as a co-activator of Nrf2-MafK and induces tumour marker gene expression during hepatocarcinogenesis [Ｊ]. Biochem J, 2007; 402(3)∶559.
7. Hernández-Alcoceba R, Sangro B, Prieto J. Gene therapy of liver cancer [Ｊ]. Ann Hepatol, 2007; 6(1)∶5.
8. Osada S, Naganawa A, Misonou M, et al. Altered gene expression of transcriptional regulatory factors in tumor marker-positive cells during chemically induced hepatocarcinogenesis [Ｊ]. Toxicol Lett, 2006; 167(2)∶106.
9. Yeh SH, Chiu CM, Chen CL, et al. Somatic mutations at the trinucleotide repeats of androgen receptor gene in male hepatocellular carcinoma [Ｊ]. Int J Cancer, 2007; 120(8)∶1610.
10. 王万祥, 欧阳晓辉, 孟兴凯, 等. 肝细胞癌组织中CT9基因mRNA表达的初步研究 [Ｊ]. 中国普外基础与临床杂志, 2006; 13(2)∶170.
11. 李新明，陶凯雄, 王国斌, 等. 黑色素瘤抗原基因-3在直肠癌的表达及意义 [Ｊ]. 中国普外基础与临床杂志, 2006; 13(3)∶308.
12. Bignotti E, Tassi RA, Calza S, et al. Differential gene expression profiles between tumor biopsies and short-term primary cultures of ovarian serous carcinomas: identification of novel molecular biomarkers for early diagnosis and therapy [Ｊ]. Gynecol Oncol, 2006; 103(2)∶405.
13. Giri D. Gene microarrays in tumor diagnosis: opportunities and challenges [Ｊ]. Med Health R I, 2005; 88(7)∶212.
14. Hatakeyama H, Akita H, Kogure K, et al. Development of a novel systemic gene delivery system for cancer therapy with a tumor-specific cleavable PEG-lipid [Ｊ]. Gene Ther, 2007; 14(1)∶68.
15. Bazan-Peregrino M, Seymour LW, Harris AL. Gene therapy targeting to tumor endothelium [Ｊ]. Cancer Gene Ther, 2007; 14(2)∶117.
16. Pastorakova A, Hlubinova K, Jakubikova J, et al. Combine cancer gene therapy harnessing plasmids expressing human tumor necrosis factor alpha and Herpes simplex thymidine kinase suicide gene [Ｊ]. Neoplasma, 2006; 53(5)∶353.
17. Ternovoi VV, Curiel DT, Smith BF, et al. Adenovirus-mediated p53 tumor suppressor gene therapy of osteosarcoma [Ｊ]. Lab Invest, 2006; 86(8)∶748.
18. 周建波, 李靖, 梁平, 等. 肝细胞癌高表达新基因BC047440功能的研究 [Ｊ]. 中华实验外科杂志, 2006; 23（3）∶288.
19. 赵弘智, 梁平, 李靖, 等. 肝癌相关cDNA片段的快速克隆和表达 [Ｊ]. 世界华人消化杂志, 2004; 12（8）∶1785.
20. Strausberg RL, Feingold EA, Grouse LH, et al. Generation and initial analysis of more than 15 000 full-length human and mouse cDNA sequences [Ｊ]. Proc Natl Acad Sci USA, 2002; 99(26)∶16899.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Expression and Significance of A Novel Gene BC047440 in Multiplicate Malignant Tumor Tissues

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