Dual Cuff Combined with Abdominal Aorta Connected with Y-Tube to Establish A Pancreaticoduodenal TransplantationModel in Rats_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

1. Department of Gast rointestinal Surgery , The Fi rst A f f i l iated Hos pi tal , Kunming Medical Col lege , Kunming 650032 , China;;
2. Department of Hepatobi l iary Pancreatic S urgery , The S econd A f f i l iated Hos pi tal , Kunming Medical Col lege , Kunming 630031 , China;;
3. The Fi rst People’s Hos pi tal of Kunming Ci t y , Kunming 650011 , China;

Corresponding author：

Keywords：

Rat,Pancreas t ransplantation,Ischemia-reperfusion injury ,Animal model

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【Abstract】Objective On the basis of traditional transplantation model, a successful model of pancreaticoduodenal transplantation (PDT) were established in rats, which is the foundation of basic and clinical transplantation research. Methods We improved the technique of microoperation on donor and harvested high-quality graft. The dual cuff technique was applied to end-to-end anastomose proximal part of abdominal aorta and portal vein with left renal aorta and vein of recipient, and distal part of abdominal aorta was connected with Y-tube. External secretion was performed by duodenum stoma. The PDT model was finished without blocking systemic circulation and portal vein system. Random blood glucose levels and drainage were monitored postoperatively to evaluate the function of endocrine and ectocrine. Results Thirty operations were done. The total procedure of transplantation lasted 2 hours. Moreover the operation on recipient and the reconstruction of vessels took only (26±5) and (25±5) minutes, respectively. The success rate was elevated to 100%. The ectocrine function was restored within 2 hours after operation. Except for 3 cases of non-function graft because of thrombosis in cannula, the glucose level of the remaining recipients was reduced to normal level 6 h or 24 h after transplantation. The survival rate of graft function was 90% (27/30). Conclusion This model is finished without special equipment and can recover the endocrine function in advance. It is a simple and stable model, which might be used in research of the theoretical problems involved in clinical pancreas transplantation.

Citation： DIAO Chang ,W EI X iaoping,L I L i,XU J ianbiao .. Dual Cuff Combined with Abdominal Aorta Connected with Y-Tube to Establish A Pancreaticoduodenal TransplantationModel in Rats. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2008, 15(4): 254-257. doi: Copy

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2. 　　Lee S, Tung KS, Koopmans H, et al. Pancreaticoduodenal transplantation in the rat [J]. Transplantation, 1972; 13(4)∶421.
3. 　Masaki Y, Suzuki K, Yan H, et al. Quantitative aspects of microchimerism after rat small bowel and pancreaticoduodenal transplantation [J]. Transplant Proc, 2000; 32(7)∶2483.
4. 　Jiga LP, Nita G, Dornean V, et al. Experimental model of pancreas transplantation with portal or systemic drainage in the laboratory rat [J]. Chirurgia (Bucur), 2007; 102(5)∶563.
5. 　朱兴国, 田清水, 陈彦, 等. 大鼠胰十二指肠移植动物模型的制作 [J]. 中华实验外科杂志, 2005; 22(4)∶496.
6. 　王为忠, 刘小南, 李开宗, 等. 大鼠单纯胰腺移植手术技术 [J]. 中国普外基础与临床杂志, 2005; 12(2)∶147.
7. 　Reddy KS, Shokouh-Amiri H, Stratta RJ, et al. Successful reuse of portal-enteric technique in pancreas retransplantation [J]. Transplantation, 2000; 69(11)∶2443.
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10. 　朱晓峰 , 王吉甫 . 不阻断腹主动脉及下腔静脉的大鼠胰腺移植 [J]. 中华显微外科杂志, 1995; 18(3)∶212.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Dual Cuff Combined with Abdominal Aorta Connected with Y-Tube to Establish A Pancreaticoduodenal TransplantationModel in Rats

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