Influence of Hyperlipemia on Rats with Acute Pancreatitis During Pregnancy_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

LIU Xingmu , WENJun , LI Yongsheng , YANG Shaohua , CHEN Shaoyi , SHEN Wenl

Department of General Surgery, The Second Affiliated Hospital of Medical College of Shantou University, Shantou 515041, China;

Corresponding author：

Keywords：

Hyperlipemia; 　　Pregnancy; 　　Acute pancreatitis; 　　L-arginine

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Objective To study the influence of hyperlipemia on rats with acute pancreatits during pregnancy and its mechanism. Methods Seventy two pregnant Sprague-Dawley rats were randomly divided into the test group and the control group， and then they were fed with high fat diet and balanced diet for 16 days separately. Pregnant rats were given intraperitoneal injection with L-arginine for 2 times (one time is 250 mg/100 g, the other is 200 mg/100 g) at an interval of 1 h. The serum triglyceride (TG), serum amylase (AMS), and lipase (LPS) from blood samples were tested just after injection, and 12 h， 18 h， 24 h， 30 h and 48 h after injection respectively, and wet/dry ratio of pancreas were measured. The histopathological score of pancreatic tissue was evaluated based on microscopic changes, and the expression of TNF-α protein was determined by SP immunohistochemical technique. Results After the last injection, the level of TG in the test group was obviously higher than that in the control group in each time (P＜0.05). The peak values of AMS and LPS in the test group appeared at 24， 18 h respectively, while the peaks appeared at 30， 24 h in the control group, respectively, which were significantly lower than those in the test group (P＜0.05). Compared with the control group, the wet/dry ratio of pancreas in the test group increased at 12, 18 and 24 h after injection (P＜0.05); The pathological changes of pancreas in test group was more serious with higher histopathological score at 0, 12, 18 and 24 h (P＜0.05), and expression of the TNF-α protein was higher at 12, 18 and 24 h (P＜0.05), too. Conclusion Hyperlipemia can make L-arginine-induced-acute-pancreatitis during pregnancy earlier occur and lead to more serious injury in pancreas. This study demonstrates that hyperlipemia may be a high risk factor for acute pancreatitis during pregnancy, making a great amount of free fatty acid released from TG and up-regulated the expression of TNF-α.

Citation： LIU Xingmu,WENJun,LI Yongsheng,YANG Shaohua,CHEN Shaoyi,SHEN Wenl. Influence of Hyperlipemia on Rats with Acute Pancreatitis During Pregnancy. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2008, 15(5): 342-346. doi: Copy

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8.	Ko CW, Sekijima JH, Lee SP, et al. Biliary sludge [Ｊ]. Ann Intern Med, 1999; 130(4 Pt 1)∶ 301.
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13.	余文，吴伟，张少辉，等. 急性胰腺炎发病机理的研究进展 [Ｊ]. 中国普外基础与临床杂志, 2006; 13(6)∶ 733.
14.	Tani S, Itoh H, Okabayashi Y, et al. New model of acute necrotizing pancreatitis induced by excessive doses of arginine in rats [Ｊ]. Dig Dis Sci, 1990; 35(3)∶ 367.
15.	Czakó L, Takács T, Varga IS, et al. The pathogenesis of L-arginine-induced acute necrotizing pancreatitis: inflammatory mediators and endogenous cholecystokinin [Ｊ]. J Physiol Paris, 2000; 94(1)∶ 43.
16.	Brivet FG, Emilie D, Galanaud P, et al. Pro- and anti-inflammatory cytokines during acute severe pancreatitis: an early and sustained response, although unpredictable of death. Parisian Study Group on Acute Pancreatitis [Ｊ]. Crit Care Med, 1999; 27(4)∶ 749.
17.	Pooran N, Indaram A, Singh P， et al. Cytokines (IL-6, IL-8, TNF): early and reliable predictors of severe acute pancreatitis [Ｊ]. J Clin Gastroenterol, 2003; 37(3)∶ 263.
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19.	杨植，杨志英，唐伟松，等. 肿瘤坏死因子在猴急性胰腺炎中的作用及生长抑素的治疗效果 [Ｊ]. 普外基础与临床杂志， 1996; 3（1）∶ 4.
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1. Kingsnorth A, O’Reilly D. Acute pancreatitis [Ｊ]. BMJ, 2006; 332(7549)∶ 1072.
2. Toskes PP. Hyperlipidemic pancreatitis [Ｊ]. Gastroenterol Clin North Am, 1990; 19(4)∶ 783.
3. De Chalain TM, Michell WL, Berger GM. Hyperlipidemia, pr-egnancy and pancreatitis [Ｊ]． Surg Gynecol Obstet, 1988; 167(6)∶ 469.
4. Yadav D, Pitchumoni CS. Issues in hyperlipidemic pancreatitis [Ｊ]. J Clin Gastroenterol, 2003; 36(1)∶ 54.
5. Schmidt J, Rattner DW, Lewandrowski K, et al. A better model of acute pancreatitis for evaluating therapy [Ｊ]. Ann Surg, 1992; 215(1)∶ 44.
6. Terzhumanov R, Uchikov A, Uchikova E, et al. Acute pancreatitis and pregnancy-analysis of a 10 year period of time [Ｊ]. Akush Ginekol (Sofiia), 2004; 43(7)∶ 9.
7. 邓明明，李云，唐世孝. 伴血脂过高的急性胰腺炎14例临床分析 [Ｊ]. 四川医学， 2002； 23（11）∶1180.
8. Ko CW, Sekijima JH, Lee SP, et al. Biliary sludge [Ｊ]. Ann Intern Med, 1999; 130(4 Pt 1)∶ 301.
9. 杨体雄，周亚魁，何跃明，等. 高甘油三酯血症对急性胰腺炎的发生及其病理损害程度的影响 [Ｊ]. 中华实验外科杂志， 2001; 18（6）∶ 544.
10. Swoboda K, Derfler K, Koppensteiner R, et al. Extracorporeal lipid elimination for treatment of gestational hyperlipidemic pancreatitis [Ｊ]. Gastroenterology, 1993; 104(5)∶ 1527.
11. Nagai H, Henrich H, Wünech PH, et al. Role of pancreatic enzymes and their substrates in autodigestion of the pancreas. In vivo studies with isolated rat pancreatic acini [Ｊ]. Gastroenterology, 1989; 96(3)∶ 838.
12. Spannagel AW, Nakano I, Tawil T, et al. Adaptation to fat markedly increases pancreatic secretory response to intraduodenal fat in rats [Ｊ]. Am J Physiol, 1996; 270(1 Pt 1)∶ G128.
13. 余文，吴伟，张少辉，等. 急性胰腺炎发病机理的研究进展 [Ｊ]. 中国普外基础与临床杂志, 2006; 13(6)∶ 733.
14. Tani S, Itoh H, Okabayashi Y, et al. New model of acute necrotizing pancreatitis induced by excessive doses of arginine in rats [Ｊ]. Dig Dis Sci, 1990; 35(3)∶ 367.
15. Czakó L, Takács T, Varga IS, et al. The pathogenesis of L-arginine-induced acute necrotizing pancreatitis: inflammatory mediators and endogenous cholecystokinin [Ｊ]. J Physiol Paris, 2000; 94(1)∶ 43.
16. Brivet FG, Emilie D, Galanaud P, et al. Pro- and anti-inflammatory cytokines during acute severe pancreatitis: an early and sustained response, although unpredictable of death. Parisian Study Group on Acute Pancreatitis [Ｊ]. Crit Care Med, 1999; 27(4)∶ 749.
17. Pooran N, Indaram A, Singh P， et al. Cytokines (IL-6, IL-8, TNF): early and reliable predictors of severe acute pancreatitis [Ｊ]. J Clin Gastroenterol, 2003; 37(3)∶ 263.
18. 王昌成，马兴刚，徐淮，等. 血清IL-6、 IL-8和TNF-α在早期诊断重症急性胰腺炎中的价值 [Ｊ]. 中华急诊医学杂志， 2001; 10（4）∶ 252.
19. 杨植，杨志英，唐伟松，等. 肿瘤坏死因子在猴急性胰腺炎中的作用及生长抑素的治疗效果 [Ｊ]. 普外基础与临床杂志， 1996; 3（1）∶ 4.
20. 田斌，李军成，刘根寿，等. 急性胰腺炎急性期血清TNF-α、 IL-6和IL-10变化的实验研究 [Ｊ]. 苏州大学学报（医学版）， 2002; 22（3）∶ 265.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Influence of Hyperlipemia on Rats with Acute Pancreatitis During Pregnancy

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