Objective To establish model of thymectomy in adult rats.
Methods The animal models were built by resection of the thymus and simultaneously emptying the air under xiphoid in the rats underwent thoracotomy.
Results Of 30 rats, 1 died of postoperative atelectasis, 1 died of excessive bleeding because of puncturing the pulmonary vein by mistake during the operation. Twenty-eight rats survived more than 30 days. A successful rate of 93.3% was achieved in the making of thymectomy model.
Conclusion The results show that the model is easy to operate and the success rate is very high, and can be used in the experiment of thymectomy in the rats.
Citation：
WANG Qian,LI Li,CHEN Gang,LI Xiaoyan,YAN Hongxian. Method of Establishing The Model of Thymectomy in Adult Rats. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2008, 15(8): 579-580. doi:
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- 1. Nakatsuji T. Rat thymectomy effects on leptin receptor and T-bet; erythroid hyperplasia with maturation arrest and suppressed T-cell-mediated hepatotoxicity [J]. Lab Hematol， 2006； 12(1)∶2.
- 2. Walker KG, Jacques BC, Tweedle JR， et al. A simpler technique for open thymectomy in adult rats [J]. J Immunol Methods, 1994; 175(1)∶33.
- 3. Oluwole OO, Depaz HA, Gopinathan R, et al. Indirect allorecognition in acquired thymic tolerance: induction of donor-specific permanent acceptance of rat islets by adoptive transfer of allopeptide-pulsed host myeloid and thymic dendritic cells [J]. Diabetes, 2001； 50(7)∶1546.
- 4. 龚非力主编. 医学免疫学 [M]. 第1版. 北京： 科学出版社， 2003∶29~30.
- 5. 熊海波， 夏穗生， 温浩. 去胸腺大鼠模型的建立及改进 [J]. 中华器官移植杂志， 2004； 25(4)∶214.
