Refinement of Heterozygosity Loss on Chromosome 7q21-22 Region in Sporadic Colorectal Cancer_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

XU Shifeng ¹ , XU Yantian ¹ , PENG Zhihai ²

1.Department of General Surgery, Hospital of Shandong Province, Jinan 250021, China;;
2.Department of General Surgery, Shanghai The First People’s Hospital, Shanghai 200080, ChinaCorresponding Author: XU Shi-feng， E-mail： xushifengdoctor@sohu.com;

Corresponding author：

Keywords：

Sporadic colorectal cancer; Loss of heterozygosity; Refinement

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Abstract Full text Figures/Tables Video References Cited by

Objective 　To refine the loss of heterozygosity (LOH) on chromosome 7q21－22 and identify the new tumor suppressor gene(s) in colorectal tumorigenesis.
Methods 　Fifteen polymorphic microsatellite markers were analyzed on chromosome 7 and another 5 markers were applied on chromosome 7q21-22 region in 83 cases of colorectal cancer and normal DNA by PCR. PCR products were electrophoresed on an ABI Prism 377 DNA sequencer. GeneScan 3.1 and Genotyper 2.1 software were used for LOH scanning and analysis.
Results 　A distinct region of frequent allelic deletion was observed on chromosome, another 5 polymorphic microsatellite markers were applied to 7q21-22 and the minimal region of frequent LOH was established on 7q21-22 spanning the D7S657, D7S646 locus.
Conclusion 　Through detailed deletion mapping studies, a critical and precise region spanning the D7S657, D7S646 locus is identified, which must contain one or more unknown tumor suppressor gene(s) on colorectal cancer.

Citation： XU Shifeng,XU Yantian,PENG Zhihai. Refinement of Heterozygosity Loss on Chromosome 7q21-22 Region in Sporadic Colorectal Cancer. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2008, 15(9): 637-641. doi: Copy

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18.	Wang Z, Shu W, Lu MM, et al. Wnt7b activates canonical signaling in epithelial and vascular smooth muscle cells through interactions with Fzd1, Fzd10, and LRP5 [Ｊ]. Mol Cell Biol, 2005; 25(12)∶5022.
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1. 　彭志海, 凌云, 周崇治, 等. 散发性结直肠癌3号染色体等位基因杂合缺失 [J]. 中华医学杂志, 2001; 81(6)∶336.
2. 　骆成玉，祝学光，李世拥, 等. nm23H1基因杂合性缺失及突变与大肠癌转移抑制 [J]. 中国普外基础与临床杂志, 1999； 6(3)∶143.
3. 　Fornasarig M, Viel A, Bidoli E, et al. Amsterdam criteria Ⅱ and endometrial cancer index cases for an accurate selection of HNPCC families [J]. Tumori, 2002; 88(1)∶18.
4. 　Xiao XY, Zhou XY, Yan G, et al. Chromosomal alteration in Chinese sporadic colorectal carcinomas detected by comparative genomic hybridization [Ｊ]. Diagn Mol Pathol, 2007; 16(2)∶96.
5. 　van Dieren JM, Wink JC, Vissers KJ, et al. Chromosomal and microsatellite instability of adenocarcinomas and dysplastic lesions (DALM) in ulcerative colitis [J]. Diagn Mol Pathol, 2006; 15(4)∶216.
6. 　Pinto AE, Roque L, Rodrigues R, et al. Frequent 7q gains in flow cytometric multiploid/hypertetraploid breast carcinomas: a study of chromosome imbalances by comparative genomic hybridisation [Ｊ]. J Clin Pathol, 2006; 59(4)∶367.
7. 　Goodison S, Viars C, Urquidi V. Molecular cytogenetic analysis of a human breast metastasis model: identification of phenotypespecific chromosomal rearrangements [Ｊ]. Cancer Genet Cytogenet, 2005; 156(1)∶37.
8. 　Hasle H, Alonzo TA, Auvrignon A, et al. Monosomy 7 and deletion 7q in children and adolescents with acute myeloid leukemia: an international retrospective study [Ｊ]. Blood, 2007; 109(11)∶4641.
9. 　Smith AG, Worrillow LJ, Allan JM. A common genetic variant in XPD associates with risk of 5q and 7qdeleted acute myeloid leukemia [Ｊ]. Blood, 2007; 109(3)∶1233.
10. 　Trovato M, Ulivieri A, Dominici R, et al. Clinicopathological significance of celltypespecific loss of heterozygosity on chromosome 7q21: analysis of 318 microdissected thyroid lesions [Ｊ]. Endocr Relat Cancer, 2004; 11(2)∶365.
11. 　Trovato M, Fraggetta F, Villari D, et al. Loss of heterozygosity of the long arm of chromosome 7 in follicular and anaplastic thyroid cancer, but not in papillary thyroid cancer [Ｊ]. J Clin Endocrinol Metab, 1999; 84(9)∶3235.
12. 　Vanharanta S, Wortham NC, Langford C, et al. Definition of a minimal region of deletion of chromosome 7 in uterine leiomyomas by tilingpath microarray CGH and mutation analysis of known genes in this region [Ｊ]. Genes Chromosomes Cancer, 2007; 46(5)∶451.
13. 　Neville PJ, Thomas N, Campbell IG. Loss of heterozygosity at 7q22 and mutation analysis of the CDP gene in human epithelial ovarian tumors [Ｊ]. Int J Cancer, 2001; 91(3)∶345.
14. 　Xia JC, Weng DS, Li JT, et al. Loss of heterozygosity analysis of a candidate gastric carcinoma tumor suppressor locus at 7q31 [Ｊ]. Cancer Genet Cytogenet, 2006; 166(2)∶166.
15. 　Chêne L, Giroud C, Desgrandchamps F, et al. Extensive analysis of the 7q31 region in human prostate tumors supports TES as the best candidate tumor suppressor gene [Ｊ]. Int J Cancer, 2004; 111(5)∶798.
16. 　Forozan F, Veldman R, Ammerman CA, et al. Molecular cytogenetic analysis of 11 new breast cancer cell lines [Ｊ]. Br J Cancer, 1999; 81(8)∶1328.
17. 　Hidalgo A, Monroy A, Arana RM, et al. Chromosomal imbalances in four new uterine cervix carcinoma derived cell lines [Ｊ]. BMC Cancer, 2003; 3(1)∶8.
18. 　Wang Z, Shu W, Lu MM, et al. Wnt7b activates canonical signaling in epithelial and vascular smooth muscle cells through interactions with Fzd1, Fzd10, and LRP5 [Ｊ]. Mol Cell Biol, 2005; 25(12)∶5022.
19. 　何渝军，何双梧，付涛. 大肠癌组织胃泌素与cmyc、cfos表达的关系 [Ｊ]. 中国普外基础与临床杂志, 2001; 8(1)∶15.
20. 　文亚渊，刘宝华，洪胜龙，等. 凋亡相关基因survivin、caspase3及cyclinB1在胃癌发生、发展中的作用 [Ｊ]. 中国普外基础与临床杂志, 2006; 13(1)∶34.
21. 　Duncan TJ, Watson NF, AlAttar AH, et al. The role of MUC1 and MUC3 in the biology and prognosis of colorectal cancer [Ｊ]. World J Surg Oncol, 2007; 9(5)∶31.
22. 　Li Y, Asuri S, Rebhun JF, et al. The RAP1 guanine nucleotide exchange factor Epac2 couples cyclic AMP and Ras signals at the plasma membrane [Ｊ]. J Biol Chem, 2006; 281(5)∶2506.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Refinement of Heterozygosity Loss on Chromosome 7q21-22 Region in Sporadic Colorectal Cancer

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