Determination of Expression of KiSS-1 Gene in Carcinoma of Gallbladder with Tissue Microarray Technique_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

Lv Zhiqiang ¹ , DING Houzhong ² , GAO Quangen ³ , QIU Jianping ³ , ZHAO Guangming ³ , NI Canrong ⁴ , YU Guanzhen ⁵

1. Clinical Medical College of Jiangsu University, Zhenjiang 212003.2. Department of General Surgery, Affiliated The First People’s Hospital of Kunshan City, Jiangsu University, Kunshan 215300,3. Affiliated The First People’s Hospital of Wujiang City, Nantong University, Wujiang 215200 .4.Department of Pathology, Affiliated Changhai Hospital;;
5.Department of Oncology, Affiliated Changzheng Hospital, The Second Military Medical University, Shanghai 200070, China;

Corresponding author：

Keywords：

Tissue microarray; KiSS-1 gene; Carcinoma of gallbladder

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Objective 　To investigate the role of KiSS-1 gene in the metastatic process of carcinoma of gallbladder and the clinicopathologic significance of KiSS-1 gene expression in carcinoma of gallbladder.
Methods 　Pathological specimens from 59 gallbladder carcinoma tissues (13 hepatic invasion and 13 lymphatic invasion tissues were included), matched with 7 para-tumor and 6 normal gallbladder tissues, were examined for the expression of KiSS-1 gene by tissue microarray technique and immunohistochemistry (EnVision).
Results 　The positive rate of KiSS-1 expression was down-regulated (P＜0.05) in tumor tissues, as compared with normal and para-tumor tissues. In carcinoma of gallbladder, the expression of KiSS-1 had no relationship with the gender, age, tumor size, histological grade or differentiation, and metastasis of lymph node, while was associated with the depth of infiltration, invasion of liver and the clinical stages (Nevin). In Ⅰ+Ⅱ, Ⅲ+Ⅳ and Ⅴ stage, the positive rates of KiSS-1 were 92.3%, 57.1% and 27.8% respectively, with an undeniably clear lowering tendency (P=0.002).
Conclusion 　 Down-regulating expression of KiSS-1 is closely associated with the processes of genesis, invasion and metastasis in carcinoma of gallbladder, and may participate in regulating these processes.

Citation： Lv Zhiqiang,DING Houzhong,GAO Quangen,QIU Jianping,ZHAO Guangming,NI Canrong,YU Guanzhen. Determination of Expression of KiSS-1 Gene in Carcinoma of Gallbladder with Tissue Microarray Technique. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2008, 15(9): 678-681. doi: Copy

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16.	Yu GZ , Wang JJ , Chen Y, et al. Significance of KiSS-1 and S100A4 in the process of metastasis of gastric carcinoma [Ｊ]. Chin-Germ J Clin Onco, 2006; 5(6)∶ 388.
17.	Sanchez-Carbayo M, Capodieci P, Cordon-Cardo C. Tumor suppressor role of KiSS-1 in bladder cancer: Loss of KiSS-1 expression is associated with bladder cancer progression and clinical outcome [Ｊ]. Am J Pathol, 2003; 162(2)∶ 609.
18.	Ringel MD, Hardy E, Bernet VJ, et al. Metastin receptor is overexpressed in papillary thyroid cancer and activates MAP kinase in thyroid cancer cells [Ｊ]. J Clin Endocrinol Metab, 2002; 87(5)∶ 2399.
19.	姜涛, 陆春雪, 张淑兰, 等. 肿瘤转移抑制基因KISS-1在子宫内膜癌组织中的表达及意义 [Ｊ]. 中国现代医学杂志, 2006; 16(13)∶ 1933.
20.	孟丽荣, 张淑兰, 鲁艳明, 等. KiSS-1基因的表达对裸鼠子宫内膜癌皮下种植瘤的影响 [Ｊ]. 中华肿瘤防治杂志, 2006; 13(23)∶ 1785.
21.	Jiang Y, Berk M, Singh LS, et al. KiSS1 suppresses metastasis in human ovarian cancer via inhibition of protein kinase C alpha [Ｊ]. Clin Exp Metastasis, 2005; 22(5)∶ 369.

1. 　杨培, 严律南, 智星, 等. 122例原发性胆囊癌外科治疗体会 [Ｊ]. 中国普外基础与临床杂志, 2004; 11(3)∶ 257.
2. 　Lee JH, Miele ME, Hicks DJ, et al. KiSS-1, a novel human malignant melanoma metastasis-suppressor gene [Ｊ]. J Natl Cancer Inst, 1996; 88(23)∶ 1731.
3. 　倪灿荣. 组织芯片的制备及在免疫组织化学中的应用 [Ｊ]. 第二军医大学学报, 2004; 25(10)∶ 1156.
4. 　梁燕, 姜军, 王清良, 等. 自制组织芯片研究VEGF-C在乳腺癌中的表达及其意义 [Ｊ]. 中国普外基础与临床杂志, 2006; 13(5)∶ 580.
5. 　Schraml P, Kononen J, Bubendorf L, et al. Tissue microarrays for gene amplification surveys in many different tumor types [Ｊ]. Clin Cancer Res, 1999; 5(8)∶ 1966.
6. 　Brlund M, Forozan F, Kononen J, et al. Detecting activation of ribosomal protein S6 kinase by complementary DNA and tissue microarrays analysis [Ｊ]. J Natl Cancer Inst, 2000; 92(15)∶ 1252.
7. 　Lee JH, Welch DR. Identification of highly expressed genes in metastasis-suppressed chromosome 6/human malignant melanoma hybrid cells using subtractive hybridization and differential display [Ｊ]. Int J Cancer, 1997; 71(6)∶ 1035.
8. 　West A, Vojta P J, Welch DR, et al. Chromosome localization and genomic structure of the KiSS-1 metastasis suppressor gene (KISS1) [Ｊ]. Genomics, 1998; 54(1)∶ 145.
9. 　Stark AM, Tongers K, Maass N, et al. Reduced metastasis-suppressor gene mRNA-expression in breast cancer brain metastases [Ｊ]. J Cancer Res Clin Oncol, 2005; 131(3)∶ 191.
10. 　Martin TA, Watkins G, Jiang WG. KiSS-1 expression in human breast cancer [Ｊ]. Clin Exp Meta, 2005; 22(6)∶ 503.
11. 　Mitchell DC, Abdelrahim M, Weng J, et al. Regulation of KiSS-1 metastasis suppressor gene expression in breast cancer cells by direct interaction of transcription factors activator protein-2 alpha and specificity protein-1 [Ｊ]. J Biol Chem, 2006; 281(1)∶ 51.
12. 　Masui T, Doi R, Mori T, et al. Metastin and its variant forms suppress migration of pancreatic cancer cells [Ｊ]. Biochem Biophys Res Commun, 2004; 315(1)∶ 85.
13. 　Ikeguchi M, Yamaguchi K, Kaibara N. Clinical significance of the loss of KiSS-1 and orphan G-protein-coupled receptor (hOT7T175) gene expression in esophageal squamous cell carcinoma [Ｊ]. Clin Cancer Res, 2004; 10(4)∶ 1379.
14. 　Dhar DK, Naora H, Kubota H, et al. Downregulation of KiSS-1 expression is responsible for tumour invasion and worse prognosis in gastric carcinoma [Ｊ]. Int J Cancer， 2004; 111(6)∶ 868.
15. 　Yu GZ, Chen Y, Ni CR, et al. Reduced protein expression of metastasis-related genes (nm23, KISS1, KAI1 and p53) in lymph node and liver metastases of gastric cancer [Ｊ]. Int J Exp Pathol, 2007; 88(3)∶ 175.
16. 　Yu GZ , Wang JJ , Chen Y, et al. Significance of KiSS-1 and S100A4 in the process of metastasis of gastric carcinoma [Ｊ]. Chin-Germ J Clin Onco, 2006; 5(6)∶ 388.
17. 　Sanchez-Carbayo M, Capodieci P, Cordon-Cardo C. Tumor suppressor role of KiSS-1 in bladder cancer: Loss of KiSS-1 expression is associated with bladder cancer progression and clinical outcome [Ｊ]. Am J Pathol, 2003; 162(2)∶ 609.
18. 　Ringel MD, Hardy E, Bernet VJ, et al. Metastin receptor is overexpressed in papillary thyroid cancer and activates MAP kinase in thyroid cancer cells [Ｊ]. J Clin Endocrinol Metab, 2002; 87(5)∶ 2399.
19. 　姜涛, 陆春雪, 张淑兰, 等. 肿瘤转移抑制基因KISS-1在子宫内膜癌组织中的表达及意义 [Ｊ]. 中国现代医学杂志, 2006; 16(13)∶ 1933.
20. 　孟丽荣, 张淑兰, 鲁艳明, 等. KiSS-1基因的表达对裸鼠子宫内膜癌皮下种植瘤的影响 [Ｊ]. 中华肿瘤防治杂志, 2006; 13(23)∶ 1785.
21. 　Jiang Y, Berk M, Singh LS, et al. KiSS1 suppresses metastasis in human ovarian cancer via inhibition of protein kinase C alpha [Ｊ]. Clin Exp Metastasis, 2005; 22(5)∶ 369.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Determination of Expression of KiSS-1 Gene in Carcinoma of Gallbladder with Tissue Microarray Technique

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