Objective To investigate the expression of transcription factor Foxp3 in the orthotopic liver transplantation by using the inbred rats with spontaneous immune tolerance.
Methods The model of orthotopic liver transplantation was established on inbred rats according to double-sleeve technique. The total RNA that was isolated from liver was reversely transcribed into cDNA. The method of real-time fluorescence quantitative PCR (RFQ-PCR) was used to analyze the expression level of Foxp3 mRNA in tolerance group and syngeneic group, respectively. The expression of Scurfin in hepatic tissue was assayed by Western blot and then was analyzed by computer imaging system.
Results The expression levels of Foxp3 mRNA and Scurfin in the transplanted liver were significantly lower than those of normal liver within the first week after transplantation. The level of Foxp3 mRNA began to increase on day 7 and reached the peak point on day 14. The expression level of Foxp3 mRNA began to decrease on day 30 but was still higher than the normal value (P＜0.05). The Western blot showed resemble changes on that of Scurfin.
Conclusion Transcription factor Foxp3 may play an important role in the spontaneous immune tolerance in the orthotopic liver transplantation of inbred rat.
Citation：
Lv Ling,ZHANG Feng,ZHANG Wei,LU Yousheng. Expression of Transcription Factor Foxp3 in Orthotopic Liver Transplantation Model of Inbred Rats with Spontaneous Immune Tolerance. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2007, 14(2): 138-141. doi:
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- 1. Meyer D, Thorwarth WM, Otto C, et al. Orthotopic liver/small bowel transplantation in rats: a microsurgical model inducing tolerance [J]. Microsurgery， 2001; 21(4)∶156.
- 2. Tu Y, Arima T, Flye MW. Rejection of spontaneously accepted rat liver allografts with recipient interleukin-2 treatment or donor irradiation [J]. Transplantation， 1997; 63(2)∶177.
- 3. Albert MH, Liu Y, Anasetti C, et al. Antigen-dependent suppression of alloresponses by Foxp3-induced regulatory T cells in transplantation [J]. Eur J Immunol， 2005; 35(9)∶2598.
- 4. Cobbold SP, Castejon R, Adams E, et al. Induction of foxP3+ regulatory T cells in the periphery of T cell receptor transgenic mice tolerized to transplants [J]. J Immunol， 2004; 172(10)∶6003.
- 5. Kamada N, Calne RY. Orthotopic liver transplantation in the rat. Technique using cuff for portal vein anastomosis and biliary drainage [J]. Transplantation， 1979; 28(1)∶47.
- 6. 王文涛, 严律南, 曾勇, 等. 大鼠肝移植模型的制作和胆道外引流方法的改进 [J]. 中国普外基础与临床杂志, 2002; 9(3)∶161.
- 7. Li X, Zhong R, He G, et al. Host immunosuppression after combined liver/intestine transplantation in the rat [J]. Transplant Proc， 1992; 24(3)∶1206.
- 8. Riordan SM, Williams R. Tolerance after liver transplantation: does it exist and can immunosuppression be withdrawn? [J]. J Hepatol, 1999; 31(6)∶1106.
- 9. Brunkow ME, Jeffery EW, Hjerrild KA, et al. Disruption of a new forkhead/winged-helix protein, scurfin, results in the fatal lymphoproliferative disorder of the scurfy mouse [J]. Nat Genet， 2001; 27(1)∶68.
- 10. Yagi H, Nomura T, Nakamura K, et al. Crucial role of FOXP3 in the development and function of human CD25+CD4+ regulatory T cells [J]. Int Immunol， 2004; 16(11)∶1643.
- 11. Taams LS, Vukmanovic-Stejic M, Smith J, et al. Antigen-specific T cell suppression by human CD4+CD25+ regulatory T cells [J].Eur J Immunol， 2002; 32(6)∶1621.
