Effect of Hepatocellular Carcinoma Cells Transfected with Inhibitory Kappa B Alpha on Expression of Nuclear Factor Kappa B and Matrix Metalloproteinase-9_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

ZHANG Kejun ¹ , GAO Yanming ² , YANG Jinyong ³ , ZHANG Bingyuan ³

Department of General Surgery, The First Affiliated Hospital, Suzhou University, Suzhou 215006, China;

Corresponding author：

Keywords：

HCC9204 cell; Cell transfected; Inhibitory kappa B alpha; Nuclear factor kappa BMatrix metalloproteinase-9

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective 　To investigate the expression changes of nuclear factor kappa B (NF-κB) and matrix metalloproteinase-9 (MMP-9) in the cultured hepatocellular carcinoma cells 9204 (HCC9204) transfected with inhibitory kappa B alpha（IκB-α)vector.
Methods 　After pcDNA3-IκB-α vector and pcDNA3 were transfected into HCC9204 by lipofectamine method, Western-blot and RT-PCR analysis were used to detect the expressions of NF-κB and MMP-9. Migration and invasion of tumor cells were assayed by fundus membrane invaded by them.
Results 　 When pcDNA3-IκB-α was transfected into HCC9204, the expression of NF-κB was decreased at the protein level, and the expression of MMP-9 mRNA and the invision and metastasis ability of transfected cells were obviously decreased.
Conclusion 　When the activity of NF-κB is inhibited, the ability of invasion and metastasis in HCC9204 cells decrease, which could be related to the decreased the expression of MMP-9.

Citation： ZHANG Kejun,GAO Yanming,YANG Jinyong,ZHANG Bingyuan. Effect of Hepatocellular Carcinoma Cells Transfected with Inhibitory Kappa B Alpha on Expression of Nuclear Factor Kappa B and Matrix Metalloproteinase-9. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2007, 14(2): 185-187. doi: Copy

1.	Shishodia S, Aggarwal BB. Nuclear factor-kappa B activation mediates cellular transformation, proliferation, invasion angiogenesis and metastasis of cancer [Ｊ]. Cancer Treat Res， 2004; 119∶139.
2.	Shukla S, MacLennan GT, Fu P, et al. Nuclear factor-kappa B/p65 (Rel A) is constitutively activated in human prostate adenocarcinoma and correlates with disease progression [Ｊ]. Neoplasia， 2004; 6(4)∶390.
3.	Andela VB, Schwarz EM, Puzas JE, et al. Tumor metastasis and the reciprocal regulation of prometastatic and antimetastatic factors by nuclear factor kappa B [Ｊ]. Cancer Res， 2000; 60(23)∶6557.
4.	Jiang Y, Cui L, Yie TA, et al. Inhibition of anchorage-independent growth and lung metastasis of A549 lung carcinoma cells by I kappa B beta [Ｊ]. Oncogene， 2001; 20(18)∶2254.
5.	Hah N, Lee ST. An absolute role of the PKC-dependent NF-kappa B activation for induction of MMP-9 in hepatocellular carcinoma cells [Ｊ]. Biochem Biophys Res Commun， 2003; 305(2)∶428.
6.	Ortega N, Jonca F, Vincent S, et al. Systemic activation of the vascular endothelial growth factor receptor KDR/flk-1 selectively triggers endothelial cells with an angiogenic phenotype [Ｊ]. Am J Pathol, 1997; 151(5)∶1215.
7.	Li Z, Ren Y, Wu QC, et al. Macrophage migration inhibitory factor enhances neoplastic cell invasion by inducing the expression of matrix metalloproteinase 9 and interleukin-8 in nasopharyngeal carcinoma cell lines [Ｊ]. Chin Med J (Engl), 2004; 117(1)∶107.
8.	张克君，高焱明，杨堂斗，等. 核因子-κB p65、IκB-α在肝炎相关性肝细胞癌中的表达及临床意义 [Ｊ]. 实用肿瘤杂志， 2004; 19(3)∶216.
9.	张克君，高焱明. 核因子-κB p65在肝炎相关性肝细胞癌中的表达及意义 [Ｊ]. 中国癌症杂志， 2005； 15（1）∶12.

1. 　Shishodia S, Aggarwal BB. Nuclear factor-kappa B activation mediates cellular transformation, proliferation, invasion angiogenesis and metastasis of cancer [Ｊ]. Cancer Treat Res， 2004; 119∶139.
2. 　Shukla S, MacLennan GT, Fu P, et al. Nuclear factor-kappa B/p65 (Rel A) is constitutively activated in human prostate adenocarcinoma and correlates with disease progression [Ｊ]. Neoplasia， 2004; 6(4)∶390.
3. 　Andela VB, Schwarz EM, Puzas JE, et al. Tumor metastasis and the reciprocal regulation of prometastatic and antimetastatic factors by nuclear factor kappa B [Ｊ]. Cancer Res， 2000; 60(23)∶6557.
4. 　Jiang Y, Cui L, Yie TA, et al. Inhibition of anchorage-independent growth and lung metastasis of A549 lung carcinoma cells by I kappa B beta [Ｊ]. Oncogene， 2001; 20(18)∶2254.
5. 　Hah N, Lee ST. An absolute role of the PKC-dependent NF-kappa B activation for induction of MMP-9 in hepatocellular carcinoma cells [Ｊ]. Biochem Biophys Res Commun， 2003; 305(2)∶428.
6. 　Ortega N, Jonca F, Vincent S, et al. Systemic activation of the vascular endothelial growth factor receptor KDR/flk-1 selectively triggers endothelial cells with an angiogenic phenotype [Ｊ]. Am J Pathol, 1997; 151(5)∶1215.
7. 　Li Z, Ren Y, Wu QC, et al. Macrophage migration inhibitory factor enhances neoplastic cell invasion by inducing the expression of matrix metalloproteinase 9 and interleukin-8 in nasopharyngeal carcinoma cell lines [Ｊ]. Chin Med J (Engl), 2004; 117(1)∶107.
8. 　张克君，高焱明，杨堂斗，等. 核因子-κB p65、IκB-α在肝炎相关性肝细胞癌中的表达及临床意义 [Ｊ]. 实用肿瘤杂志， 2004; 19(3)∶216.
9. 　张克君，高焱明. 核因子-κB p65在肝炎相关性肝细胞癌中的表达及意义 [Ｊ]. 中国癌症杂志， 2005； 15（1）∶12.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effect of Hepatocellular Carcinoma Cells Transfected with Inhibitory Kappa B Alpha on Expression of Nuclear Factor Kappa B and Matrix Metalloproteinase-9

Abstract Full text Figures/Tables Video References Cited by

Format

Content