Expression of c-met mRNA in The Drainage from Axillary Fossa after Operation for Breast Cancer_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

YANG Weijun ¹ , HUANG Zhigang ¹ , LI Chunlai ¹ , WU Zhengyang ¹ , YIN Jiajun. ²

Department of General Surgery, The First People’s Hospital of Guiyang, Guiyang 550002, China;

Corresponding author：

Keywords：

Breast cancer; Micrometastasis; RT-PCR; c-met mRNA

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective 　To study the significance of c-met mRNA in axillary drainage after operations for breast cancer.
Methods 　RT-PCR assay was used to examine c-met mRNA in axillary drainage after operations in 52 cases of breast cancer. The relationships between the expression of c-met and the tumor size, metastatic lymph nodes， the expressions of estrogen receptor (ER), progesterone receptor (PR) and c-erbB-2 were analyzed, respectively. In addition, the effect of douching operative field with 5-FU and distilled water on the expression of c-met mRNA was also analyzed.
Results 　①The proto-oncogene c-met mRNA could be detected in axillary drainage after operations for breast cancer by RT-PCR, and its positive rate was higher than that in routine pathological detection for micrometastasis in the axillary lymph nodes (P＜0.05). ②The expression of c-met mRNA was correlated with both the metastatic lymph nodes and tumor size. ③There was no significant relationship between the expression of c-met mRNA and the expressions of ER, PR and c-erbB-2. ④Dounching operative field with 5-FU and distilled water could decrease the expression of c-met mRNA.
Conclusion 　The proto-oncogene c-met mRNA may be an ideal and specific marker for dectecting micrometastasis of breast cancer. In addition, it also suggests that the examination of c-met mRNA in the axillary drainage by RT-PCR assay could detect the micrometastasis in axillary lymph nodes much easier and more accurately than routine pathological method.

Citation： YANG Weijun,HUANG Zhigang,LI Chunlai,WU Zhengyang,YIN Jiajun.. Expression of c-met mRNA in The Drainage from Axillary Fossa after Operation for Breast Cancer. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2007, 14(3): 259-262. doi: Copy

1.	傅西林，张立华，张国珩. 常规检查淋巴结转移阴性之乳腺癌腋窝组织再连续切片检查的研究 [J]. 中国肿瘤临床， 1985； 12（1）∶20.
2.	陈朝伦，卢晓梅，黄绒，等. Ⅰ期癌症患者切除淋巴结内隐匿性微小转移癌灶的检查及其临床病理意义 [J]. 中华肿瘤杂志， 1997； 19(1)∶69.
3.	Gardner B, Feldman J. Are positive axillary nodes in breast ca-ncer markers for incurable disease? [J]. Ann Surg， 1993; 218(3)∶270.
4.	Noguchi M. Sentinel lymph node biopsy and breast cancer [J]. Br J Surg， 2002; 89(1)∶21.
5.	张维铭. 现代分子生物学实验手册 [M]. 北京：科学出版社， 2003∶103~106.
6.	Tomioka D, Maehara N, Kuba K, et al. Inhibition of growth, invasion, and metastasis of human pancreatic carcinoma cells by NK4 in an orthotopic mouse model [J]. Cancer Res， 2001; 61(20)∶7518.
7.	Kermorgant S, Aparicio T, Dessirier V, et al. Hepatocyte gr-owth factor induces colonic cancer cell invasiveness via enhanced motility and protease overproduction. Evidence for PI3 kinase and PKC involvement [J]. Carcinogenesis， 2001; 22(7)∶1035.
8.	Wang Y, Selden AC, Morgan N, et al. Hepatocyte growth factor/scatter factor expression in human mammary epithelium [J]. Am J Pathol， 1994; 144(4)∶675.
9.	Yamashita J, Ogawa M, Yamashita S, et al. Immunoreactive hepatocyte growth factor is a strong and independent predictor of recurrence and survival in human breast cancer [J]. Cancer Res， 1994; 54(7)∶1630.
10.	Di Renzo MF, Narsimhan RP, Olivero M, et al. Expression of the Met/HGF receptor in normal and neoplastic human tissues [J]. Oncogene， 1991; 6(11)∶1997.
11.	Kuniyasu H, Yasui W, Kitadai Y, et al. Frequent amplification of the c-met gene in scirrhous type stomach cancer [J]. Biochem Biophys Res Commun， 1992; 189(1)∶227.
12.	Di Renzo MF, Olivero M, Ferro S, et al. Overexpression of the c-MET/HGF receptor gene in human thyroid carcinomas [J]. Oncogene， 1992; 7(12)∶2549.
13.	Liu C, Park M, Tsao MS. Overexpression of c-met proto-oncogene but not epidermal growth factor receptor or c-erbB-2 in primary human colorectal carcinomas [J]. Oncogene， 1992; 7(1)∶181.
14.	陈显玉，喻梅英，杨竹林, 等. 乳腺良恶性病变组织c-met蛋白和转化生长因子α的表达及意义 [J]. 中国普外基础与临床杂志, 1999; 6(2)∶81.
15.	尹家俊，宋燕. 肝细胞生长因子及受体c-met在乳腺癌中的研究进展 [J]. 中国普外基础与临床杂志, 2000; 7(5)∶347.
16.	Tolgay Ocal I, Dolled-Filhart M, D’Aquila TG, et al. Tissue microarray-based studies of patients with lymph node negative breast carcinoma show that met expression is associated with worse outcome but is not correlated with epidermal growth factor family receptors [J]. Cancer， 2003; 97(8)∶1841.
17.	Tsarfaty I, Alvord WG, Resau JH, et al. Alteration of Met protooncogene product expression and prognosis in breast carcinomas [J]. Anal Quant Cytol Histol， 1999; 21(5)∶397.
18.	Ghoussoub RA, Dillon DA, D’Aquila T, et al. Expression of c-met is a strong independent prognostic factor in breast carcinoma [J]. Cancer， 1998; 82(8)∶1513.
19.	徐叔云主编. 中华临床药物学 [M]. 北京：人民卫生出版社， 2003∶1429.
20.	Scorilas A, Yotis J, Stravolemos K, et al. c-erbB-2 overexpression may be used as an independent prognostic factor for breast cancer patients [J]. Anticancer Res， 1995; 15(4)∶1543.

1. 傅西林，张立华，张国珩. 常规检查淋巴结转移阴性之乳腺癌腋窝组织再连续切片检查的研究 [J]. 中国肿瘤临床， 1985； 12（1）∶20.
2. 陈朝伦，卢晓梅，黄绒，等. Ⅰ期癌症患者切除淋巴结内隐匿性微小转移癌灶的检查及其临床病理意义 [J]. 中华肿瘤杂志， 1997； 19(1)∶69.
3. Gardner B, Feldman J. Are positive axillary nodes in breast ca-ncer markers for incurable disease? [J]. Ann Surg， 1993; 218(3)∶270.
4. Noguchi M. Sentinel lymph node biopsy and breast cancer [J]. Br J Surg， 2002; 89(1)∶21.
5. 张维铭. 现代分子生物学实验手册 [M]. 北京：科学出版社， 2003∶103~106.
6. Tomioka D, Maehara N, Kuba K, et al. Inhibition of growth, invasion, and metastasis of human pancreatic carcinoma cells by NK4 in an orthotopic mouse model [J]. Cancer Res， 2001; 61(20)∶7518.
7. Kermorgant S, Aparicio T, Dessirier V, et al. Hepatocyte gr-owth factor induces colonic cancer cell invasiveness via enhanced motility and protease overproduction. Evidence for PI3 kinase and PKC involvement [J]. Carcinogenesis， 2001; 22(7)∶1035.
8. Wang Y, Selden AC, Morgan N, et al. Hepatocyte growth factor/scatter factor expression in human mammary epithelium [J]. Am J Pathol， 1994; 144(4)∶675.
9. Yamashita J, Ogawa M, Yamashita S, et al. Immunoreactive hepatocyte growth factor is a strong and independent predictor of recurrence and survival in human breast cancer [J]. Cancer Res， 1994; 54(7)∶1630.
10. Di Renzo MF, Narsimhan RP, Olivero M, et al. Expression of the Met/HGF receptor in normal and neoplastic human tissues [J]. Oncogene， 1991; 6(11)∶1997.
11. Kuniyasu H, Yasui W, Kitadai Y, et al. Frequent amplification of the c-met gene in scirrhous type stomach cancer [J]. Biochem Biophys Res Commun， 1992; 189(1)∶227.
12. Di Renzo MF, Olivero M, Ferro S, et al. Overexpression of the c-MET/HGF receptor gene in human thyroid carcinomas [J]. Oncogene， 1992; 7(12)∶2549.
13. Liu C, Park M, Tsao MS. Overexpression of c-met proto-oncogene but not epidermal growth factor receptor or c-erbB-2 in primary human colorectal carcinomas [J]. Oncogene， 1992; 7(1)∶181.
14. 陈显玉，喻梅英，杨竹林, 等. 乳腺良恶性病变组织c-met蛋白和转化生长因子α的表达及意义 [J]. 中国普外基础与临床杂志, 1999; 6(2)∶81.
15. 尹家俊，宋燕. 肝细胞生长因子及受体c-met在乳腺癌中的研究进展 [J]. 中国普外基础与临床杂志, 2000; 7(5)∶347.
16. Tolgay Ocal I, Dolled-Filhart M, D’Aquila TG, et al. Tissue microarray-based studies of patients with lymph node negative breast carcinoma show that met expression is associated with worse outcome but is not correlated with epidermal growth factor family receptors [J]. Cancer， 2003; 97(8)∶1841.
17. Tsarfaty I, Alvord WG, Resau JH, et al. Alteration of Met protooncogene product expression and prognosis in breast carcinomas [J]. Anal Quant Cytol Histol， 1999; 21(5)∶397.
18. Ghoussoub RA, Dillon DA, D’Aquila T, et al. Expression of c-met is a strong independent prognostic factor in breast carcinoma [J]. Cancer， 1998; 82(8)∶1513.
19. 徐叔云主编. 中华临床药物学 [M]. 北京：人民卫生出版社， 2003∶1429.
20. Scorilas A, Yotis J, Stravolemos K, et al. c-erbB-2 overexpression may be used as an independent prognostic factor for breast cancer patients [J]. Anticancer Res， 1995; 15(4)∶1543.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Expression of c-met mRNA in The Drainage from Axillary Fossa after Operation for Breast Cancer

Abstract Full text Figures/Tables Video References Cited by

Format

Content