Effect of Expression of bcl-2 on Renal Cell Apoptosis Induced by Obstructive Jaundice in Rats_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

LIN Lin ¹ , GONG Peng ² , MA Xiaolan ¹ , Lv Shen ² , WANG Zhongyu ²

Department of General Surgery, The Affiliated Xinhua Hospital of Dalian University, Dalian 116021, China;

Corresponding author：

Keywords：

Obstructive jaundice; Renal impairment; bcl-2 protein; Cell apoptosis

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective 　To investigate the effect of renal cell apoptosis induced by obstructive jaundice on the expression of bcl-2 in rats, and to explore the mechanism of renal impairment induced by obstructive jaundice.
Methods 　Thirty-two male SD rats were randomly divided into 2 groups: SO group and BDL group. The rats in SO group received sham operation. Bile ducts of rats in BDL group were ligated. Pathology of kidneys was observed under the microscope. The levels of D-Bil, TBA, GOT, GPT, Cr and BUN in serum and β2-MG in urine were measured. The apoptotic rate of renal cells was calculated by flow cytometry and the forms of DNA fragmentation in renal cells were detected by agarose gel electrophoresis. The expression of inhibitory gene bcl-2 in the renal tissues was detected by immunohistochemistry.
Results 　The color of urine in BDL group became dark yellow in day 2 after operation; The ears, tails and the muscle of abdominal wall and splanchnic organs, such as liver and kidney, also became yellow and swollen in day 7. The D-Bil, TBA, GOT, GPT, BUN of serum and β2 -MG of urine in BDL group were higher than those in SO group (P＜0.05, P＜0.01), and each value (except β2 -MG) in BDL group of 14 d was higher than that in BDL group of 7 d (P＜0.05, P＜0.01), respectively. The result of flow cytometry showed that the apoptotic rate of SO group and BDL (7 d and 14 d) group were (2.10±0.75)%， (18.17±0.86)% and (36.39±2.23)% respectively, there were significantly difference among them (P＜0.05). The expression rate of bcl-2 of renal cell in BDL group of 7 d was higher than that in BDL group of 14 d.
Conclusion 　Obstructive jaundice could induce apoptosis of the renal cells, and activate the expression of bcl-2 of the renal tubular epithelial cells in feedback, which may regulate the process of apoptosis.

Citation： LIN Lin,GONG Peng,MA Xiaolan,Lv Shen,WANG Zhongyu. Effect of Expression of bcl-2 on Renal Cell Apoptosis Induced by Obstructive Jaundice in Rats. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2007, 14(3): 296-299. doi: Copy

1.	巩鹏, 王忠裕, 李克军, 等. 胆道梗阻后心肌损伤的实验研究 [J]. 肝胆胰外科杂志, 2002; 14(3)∶157.
2.	梁培禾，靳风烁，江军，等. 大鼠急性肾功能衰竭时肾小管上皮细胞Fas、 FasL表达的意义 [J]. 创伤外科志, 2003; 5(2)∶118.
3.	张丰深, 何振平, 段恒春. 梗阻性黄疸并发肾功能损害的实验研究 [J]. 第三军医大学学报, 2002; 24(9)∶1046.
4.	Jaeschke H, Gores GJ, Cederbaum AI, et al. Mechanisms of hepatotoxicity [J]. Toxicol Sci， 2002; 65(2)∶166.
5.	张健生, 胡稳心. 梗阻性黄疸患者微循环与肾功能改变及其意义 [J]. 陕西医学杂志, 1992; 21(6)∶360.
6.	Stahelin BJ, Marti U, Zimmermann H, et al. The interaction of Bcl-2 and Bax regulates apoptosis in biliary epithelial cells of rats with obstructive jaundice [J]. Virchows Arch， 1999; 434(4)∶333.
7.	Korsmeyer SJ. Bcl-2 initiates a new category of oncogenes: regulators of cell death [J]. Blood， 1992; 80(4)∶879.
8.	Bairaktari E, Liamis G, Tsolas O, et al. Partially reversible renal tubular damage in patients with obstructive jaundice [J]. Hepatology， 2001; 33(6)∶1365.

1. 巩鹏, 王忠裕, 李克军, 等. 胆道梗阻后心肌损伤的实验研究 [J]. 肝胆胰外科杂志, 2002; 14(3)∶157.
2. 梁培禾，靳风烁，江军，等. 大鼠急性肾功能衰竭时肾小管上皮细胞Fas、 FasL表达的意义 [J]. 创伤外科志, 2003; 5(2)∶118.
3. 张丰深, 何振平, 段恒春. 梗阻性黄疸并发肾功能损害的实验研究 [J]. 第三军医大学学报, 2002; 24(9)∶1046.
4. Jaeschke H, Gores GJ, Cederbaum AI, et al. Mechanisms of hepatotoxicity [J]. Toxicol Sci， 2002; 65(2)∶166.
5. 张健生, 胡稳心. 梗阻性黄疸患者微循环与肾功能改变及其意义 [J]. 陕西医学杂志, 1992; 21(6)∶360.
6. Stahelin BJ, Marti U, Zimmermann H, et al. The interaction of Bcl-2 and Bax regulates apoptosis in biliary epithelial cells of rats with obstructive jaundice [J]. Virchows Arch， 1999; 434(4)∶333.
7. Korsmeyer SJ. Bcl-2 initiates a new category of oncogenes: regulators of cell death [J]. Blood， 1992; 80(4)∶879.
8. Bairaktari E, Liamis G, Tsolas O, et al. Partially reversible renal tubular damage in patients with obstructive jaundice [J]. Hepatology， 2001; 33(6)∶1365.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effect of Expression of bcl-2 on Renal Cell Apoptosis Induced by Obstructive Jaundice in Rats

Abstract Full text Figures/Tables Video References Cited by

Format

Content