Effects of Local Content of Nitrogen Monoxidum on Bile Reflux in Rats with Pylori Stress Ulcer_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

CHEN Min ¹ , WEI Wei ² , WEN Rui ³ , TAO Chen ¹ , ZHANG Xiaoyu ¹ , ZENG Wenquan ¹ , ZHAO Wei ¹ , AI Min. ¹

The Fourth Department of Hepatology， The Second Hospital of Nanjing City, Nanjing 210003, China;

Corresponding author：

Keywords：

Stress ulcer; Nitrogen monoxidum; Bile reflux

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Objective 　To investigate the effects and possible mechanism of the local content changes of nitrogen monoxidum on bile reflux in rats’ pylori stress ulcer.
Methods 　Fifty adult SD rats were restrained in planes and then were soaked in cold water to make stress ulcer. They were divided into three groups randomly: the stress group (30 cases), the L-NAME group (10 cases), whose pylori were injected with L-NAME solution and the normal saline group (10 cases), whose pylori were treated with saline solution. Besides, there were another 10 rats taken as the control group. The gastric ulcer index, pH value and bile acid concentration of gastric juice in each group were measured before and after stress. The local concentrations of nitrogen monoxidum in rats’ pylori were also measured by nitrogen monoxidum Biochemical Detection Kit.
Results 　The local content of nitrogen monoxidum in rats’ pylori increased to maximum at 1 h after stress （P＜0.01）, while the bile acid and pH value of gastric juice went up to the maximum at 2 h after stress （P＜0.01）. The ulcer index reached the peak value at 4 h after stress （P＜0.01）. The bile acid concentration and ulcer index of the group injected with L-NAME solution in rats’ pylori decreased significantly compared with those of the group treated with normal saline solution (P＜0.01). The local concentration of nitrogen monoxidum also decreased significantly in the L-NAME group (P＜0.05）. However, there was no difference on pH values of gastric juice between those two groups (P gt;0.05).
Conclusion 　There is a positive correlation between the local concentration of nitrogen monoxidum in pylori and bile acid in the stomach in rats with stress ulcer. Injection of L-NAME solution in rats’ pylori may reduce the local content of nitrogen monoxidum so that the pyloric sphincter become less tense, which may lead to the significant decreases of ulcer index and bile acid in the stomach. Thus, L-NAME may protect gastric mucus effectively.

Citation： CHEN Min,WEI Wei,WEN Rui,TAO Chen,ZHANG Xiaoyu,ZENG Wenquan,ZHAO Wei,AI Min.. Effects of Local Content of Nitrogen Monoxidum on Bile Reflux in Rats with Pylori Stress Ulcer. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2007, 14(3): 300-303. doi: Copy

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15.	Tepperman BL, Whittle BJ. Endogenous nitric oxide and sensory neuropeptides interact in the modulation of the rat gastric microcirculation [J]. Br J Pharmacol, 1992; 105(1)∶171.
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22.	Kudo T, Shimoda H, Usui T, et al. Organization of nitric oxide-producing nerves in the rat pyloric sphincter [J]. Arch Histol Cytol, 1998; 61(4)∶361.

1. 李勋，余安国，邓镇平，等. 大鼠应激性溃疡发病机理及防治研究 [J]. 中国普外基础与临床杂志, 2000; 7（3）∶138.
2. 朱爱勇，许国铭，李兆申，等. 慢性胃炎患者胃内24小时胆汁监测 [J]. 中华消化杂志, 2002; 22（9）∶530.
3. 谭东毅，姚宏昌. 功能性消化不良的胃内24小时胆汁监测和胃动力研究 [J]. 中华消化杂志, 2002; 22（5）∶313.
4. 窦勇鹰，谢立群，李俊美，等. 蛋白酶激活受体-2激动剂在应激性溃疡中的作用 [J]. 世界华人消化杂志， 2006； 14（34）∶3306.
5. 李兆申, 朱爱勇, 许国铭，等. 胆汁反流与胃内pH值的关系 [J]. 解放军医学杂志, 2001; 26（10）∶708.
6. 陈仕珠，赵红，吴春艳，等. 胆汁返流性胃炎患者胆囊排空功能研究 [J]. 世界华人消化杂志, 1998; 6∶427.
7. 陈胜良, 莫剑忠, 陈晓宇，等. 胆汁反流、胃酸和幽门螺杆菌感染共同作用对胃黏膜损伤程度和分布的影响 [J]. 胃肠病学, 2002; 7（5）∶280.
8. Wang X, Zhong YX, Zhang ZY, et al. Effect of L-NAME on nitric oxide and gastrointestinal motility alterations in cirrhotic rats [J]. World J Gastroenterol, 2002; 8(2)∶328.
9. 聂时南, 李兆申, 湛先保，等. 一氧化氮合酶在心理性应激胃黏膜损伤中作用 [J]. 上海医学, 2002; 25（5）∶302.
10. Fan YP, Chakder S, Gao F, et al. Inducible and neuronal nitric oxide synthase involvement in lipopolysaccharide-induced sphincteric dysfunction [J]. Am J Physiol Gastrointest Liver Physiol, 2001; 280(1)∶G32.
11. 崔忠敏，李兆申，许国铭，等. 左旋精氨酸甲酯及左旋精氨酸对应激状态下胃黏膜耐受性细胞保护作用的影响 [J]. 中华消化杂志, 2001; 21（4）∶219.
12. Arakawa T, Uno H, Fukuda T, et al. New aspects of gastric adaptive relaxation, reflex after food intake for more food: involvement of capsaicin-sensitive sensory nerves and nitric oxide [J]. J Smooth Muscle Res, 1997; 33(3)∶81.
13. 瞿颂义, 李伟, 郑天珍. 一氧化氮在胃肠运动及胃肠病理中的作用 [J]. 生理科学进展, 1995; 26(1)∶77.
14. Willis S, Allescher HD, Weigert N, et al. Influence of the L-arginine-nitric oxide path way on vasoactive intestinal polypeptide release and motilily in the rat stomach in vitro [J]. Eur J Pharmacol, 1996; 315(1)∶59.
15. Tepperman BL, Whittle BJ. Endogenous nitric oxide and sensory neuropeptides interact in the modulation of the rat gastric microcirculation [J]. Br J Pharmacol, 1992; 105(1)∶171.
16. Mashimo H, Kjellin A, Goyal RK. Gastric stasis in neuronal nitric oxide synthase-deficient knockout mice [J]. Gastroenterology, 2000; 119(3)∶766.
17. 于风海, 李兆申, 许国铭. 一氧化氮, 血管活性肠肽与反流性食管炎 [J]. 中华消化杂志, 2001; 21(7)∶430.
18. Bayguinov O, Sanders KM. Role of nitric oxide as an inhibitory neurotransmitter in the canine pyloric sphincter [J]. Am J Physiol, 1993; 264(5 Pt 1)∶G975.
19. Grisoni E, Dusleag D, Super D. Nitric oxide synthesis inhibition: the effect on rabbit pyloric muscle [J]. J Pediatr Surg, 1996; 31(6)∶800.
20. Qui BS, Mei QB, Liu L, et al. Effects of nitric oxide on gastric ulceration induced by nicotine and cold-restraint stress [J]. World J Gastroenterol, 2004; 10(4)∶594.
21. Tomita R, Tanjoh K, Fujisaki S, et al. The role of nitric oxide (NO) in the human pyloric sphincter [J]. Hepatogastroenterology, 1999; 46(29)∶2999.
22. Kudo T, Shimoda H, Usui T, et al. Organization of nitric oxide-producing nerves in the rat pyloric sphincter [J]. Arch Histol Cytol, 1998; 61(4)∶361.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effects of Local Content of Nitrogen Monoxidum on Bile Reflux in Rats with Pylori Stress Ulcer

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