Influence of Radix Salviae Miltiorrhizae Injection upon NF-κB in Rat with Severe Acute Pancreatitis_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

ZHENG Bangrui , SHEN Wenlu , WEN Jun , YANG Shaohua , CHEN Shaoyi

Department of General Surgery, The Second Affiliated Hospital of Medical College of Shantou University, Shantou 515041, China;

Corresponding author：

Keywords：

Severe acute pancreatitis; Nuclear factor-kappa B; Radix salviae miltiorrhizae injection

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Objective 　To investigate the mechanism of radix salviae miltiorrhizae (RSM) injection in treatment of pancreatitis through observing the changes of activity of nuclear factor-kappa B (NF-κB) in pancreas of rats with severe acute pancreatitis (SAP) and the influence of RSM injection upon NF-κB in pancreas tissue.
Methods 　Seventy-five rats were randomly divided into 3 groups: normal control group, SAP group and RSM treatment group, which were injected with normal saline, normal saline or RSM in the peritoneal cavity, respectively. The model of SAP rats was made by injecting L-arginine into peritoneal cavity and by subcutaneous injection at the same time. The concentrations of amylase in plasma and in ascites were measured respectively, and the expression of NF-κB in pancreas tissues was determined by immunohistochemistry.
Results 　The levels of amylase in plasm and ascites in SAP group and RSM treatment group increased significantly with the increased expression of NF-κB in pancreas tissue, but it was also found that both the level of amylase and the expression of NF-κB in RSM treatment group were significantly lower compared with those in SAP group, and the survival time of RSM treatment group was longer than SAP group with less pathological injury in the pancreas tissues.
Conclusion 　RSM may be effective for the treatment of pancreatitis by degrading the expression of NF-κB.

Citation： ZHENG Bangrui,SHEN Wenlu,WEN Jun,YANG Shaohua,CHEN Shaoyi. Influence of Radix Salviae Miltiorrhizae Injection upon NF-κB in Rat with Severe Acute Pancreatitis. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2007, 14(4): 392-395. doi: Copy

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1. 　孙志明. 重症急性胰腺炎的诊断治疗现状[J]. 新疆医学， 2006; 36(2)∶121.
2. 　余文，吴伟，张少辉，等. 急性胰腺炎发病机理的研究进展[J]. 中国普外基础与临床杂志， 2006； 13（6）∶733.
3. 　刘素芳，曹亚. NF-κB的激活及阻断策略的研究进展 [J]. 国际病理科学与临床杂志, 2005; 23(3)∶80.
4. 　程国祚, 张建新, 李龙，等. 急性坏死性胰腺炎大鼠肾损害与肾微循环的动态变化 [J]. 肝胆外科杂志， 2002; 8(4)∶36.
5. 　孙雷. 急性胰腺炎发病机制研究进展 [J]. 中华肝胆外科杂志， 2004； 12（10）∶853.
6. 　Steinle AU, Weidenbach H, Wagner M, et al. NF-kappaB/Rel activation in cerulein pancreatitis[J]. Gastroenterology， 1999; 116(2)∶420.
7. 　Folch-Puy E, Granell S, Dagorn JC, et al. Pancreatitis-associated protein I suppresses NF-kappa B activation through a JAK/STAT-mediated mechanism in epithelial cells[J]. J Immunol， 2006; 176(6)∶3774.
8. 　Dlugosz JW, Andrzejewska A, Nowak K, et al. The cumulative effect of nuclear factor-kappaB (NF-kappaB) inhibition and endothelins in early cerulein-induced acute pancreatitis in rats[J]. Rocz Akad Med Bialymst， 2005; 50(6)∶230.
9. 　孙亚红. 丹参在治疗重症急性胰腺炎中的运用[J]. 现代中西医结合杂志, 2006; 15(21)∶2923.
10. 　张建新，程国祚，李龙，等. 丹参对急性胰腺炎大鼠肠微循环的影响及其保护作用[J]. 中华肝胆外科杂志， 2002; 8(12)∶50.
11. 　刘天舒，朱理伟，王鹏志，等. 急性重症胰腺炎大鼠细胞凋亡的研究 [J]. 中华实验外科杂志， 2002； 19（4）∶298.
12. 　Nolan B, Collete H, Baker S, et al. Inhibition of neutrophil apoptosis after severe trauma is NF kappa beta dependent [J]. J trauma, 2000; 48(4)∶599.
13. 　段美丽，张淑文，王宝恩. 急性胰腺炎患者并发多器官功能障碍综合征的机制探讨 [J]. 临床和实验医学杂志， 2002； 1（2）∶66.
14. 　李文星, 王锋, 韩德五，等. 重症急性胰腺炎时肠源性内毒素血症与TNF-α、 IL-1β、 NO及MDA之间的相关性研究[J]. 山西医科大学学报， 2006; 37(9)∶27.
15. 　石力，田伏洲，李旭，等. 急性胰腺炎大鼠肝脏NF-κB对TNF-α表达的调控及其在肝损伤中的作用 [J]　. 中国普外基础与临床杂志， 2004； 11（3）∶228.
16. 　陆逢春，黄鹤光. 肿瘤坏死因子α对重症急性胰腺炎低钙血症的影响及作用机制 [J]. 中华普通外科杂志， 2006; 22(11)∶83.
17. 　Long J, Song N, Liu XP, et al. Nuclear factor-kappaB activation on the reactive oxygen species in acute necrotizing pancreatitic rats[J]. World J Gastroenterol， 2005; 11(27)∶4277.
18. 　马志胜，沈文律. 中药丹参、生脉治疗全身炎症反应综合症的实验研究 [J]. 中国普外基础与临床杂志， 2005； 12（4）∶395.
19. 　张喜平，陆贝. 核因子-κB在重症急性胰腺炎发病过程及MODS中的作用[J]. 医学研究杂志, 2006; 35(11)∶87.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Influence of Radix Salviae Miltiorrhizae Injection upon NF-κB in Rat with Severe Acute Pancreatitis

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