LIU Ziming , YAN Lunan , LI Xinxiao
  • Department of General Surgery, West China Hospital, Sichuan University, Chengdu 610041, China;
Gene therapy; Biodegradable microsphere; Adenovirus; HSV-TK gene; Liver cancer
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Objective 　To evaluate the suitability of the biodegradable microsphere encapsulation of adenovirus as a targeting vector for gene therapy of hepatocellular carcinoma.
Methods 　Encapsulate the recombinant adenovirus in PLG 〔poly (lactic/glycolic)〕 copolymer by the solution evaporation method, the release test and the bioactivity of viruses incorporated in vitro were studied.
Results 　More than 19.3% of adenovirus was encapsulated in PLG microspheres. The release test shows that the adenovirus was released for more than 200 h, 50% were shed within the first 100 h, and their activity was retained.
Conclusion 　Recombinant adenovirus can be formulated in a polymer preparation of PLG with retention of bioactivity. It may be a valuable vector for the gene therapy of liver cancer.

Citation： LIU Ziming,YAN Lunan,LI Xinxiao. Microsphere Preparation of The Recombinant Adeno-Associated Virus as A Vector for Gene Therapy of Liver Cancer. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2007, 14(4): 399-402. doi: Copy