• Department of General Surgery, West China Hospital, Sichuan University, Chengdu 610041, China;
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Objective   To explore the effects of overexpression of human tissue inhibitors of metalloproteinase-1 (hTIMP-1) on proliferation of human liver cancer cell line HepG2 in vitro.
Methods  A recombinant adenoviral vector containing full-length cDNA of hTIMP-1 was generated and transfected into HepG2. The viral titer was checked by measuring GFP, and the expression of hTIMP-1 in vitro was detected by the techniques of Western blot and semi-quantitative RT-PCR. The ultrastructure was observed by transmission electron microscope and the effects of overexpression of hTIMP-1 on proliferation of HepG2 in vitro was analyzed by MTT assay and growth curve.
Results  The resultant AdhTIMP-1 was successfully constructed and the expression of hTIMP-1 was detected by Western blot and RT-PCR. The growth and proliferation of HepG2, which had been transfected with AdhTIMP-1,  was significantly inhibited.
Conclusion  The proliferation of HepG2 was markedly inhibited by recombinant adenovirus-mediated overexpression of hTIMP-1, which may pave the way for further application in liver gene therapy.

Citation: XIA Dong,XU Liang,WAN Liyi,WANG Yuanzheng,ZUO Huaiquan,YAN Lnan. Effects of Recombinant Adenovirus-Mediated Overexpression of hTIMP-1 on Proliferation of Human Liver Cancer Cell Line HepG2 in Vitro. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2007, 14(4): 409-412. doi: Copy