Establishment of Pancreas-Duodenal Transplantation Model in Rat with Dual Cuff Technique_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

LIU Qiyu , CHENG Ruochuan , SU Yanjun , ZHANG Jianming , DIAO Chang

Department of Gastrointestinal Surgery, The First Affiliated　 Hospital of Kunming Medical College, Kunming 650032, China;

Corresponding author：

Keywords：

Pancreas; Transplantation; Model; Rat

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective 　To establish a rat model of pancreas-duodenal transplantation for pancreas transplantation research.
Methods 　A rat model of pancreas-duodenal transplantation was established by using dual cuff technique. The graft affiliated portal vein and abdominal aorta were anastomosed to recipient’s left renal vein and left renal artery by using dual cuff technique without clamping the systemic circulation in order to minimize hemodynamic instability. Simultaneously the graft duodenum was anastomosed to the host jejunum in an end-to-side fashion to reestablish drainage of pancreatic secretion. Fluid replacement, warm keeping and anticoagulation were maintained during perioperative period.
Results 　The average donor operation time was (68.4±7.2) min and recipient operation time was (26.1±3.3) min. Moreover, it took (5.0±1.1) min for cuff preparation in vitro and (9.6±3.5) min for vessel reconstruction in vivo, respectively. Intestinal anastomosis took (7.2±2.3) min. The operation successful rate was about 91.7%.
Conclusion 　This pancreas-duodenal transplantation model avoids systemic circulation clamping. It is simple and stable, with the value in pancreas transplantation research.

Citation： LIU Qiyu,CHENG Ruochuan,SU Yanjun,ZHANG Jianming,DIAO Chang. Establishment of Pancreas-Duodenal Transplantation Model in Rat with Dual Cuff Technique. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2007, 14(4): 420-422. doi: Copy

1.	Navarro-Pinero A, Castro-Santiago MJ, Aranda-Narvaez JM, et al. Pancreatic transplantation. Results of the Malaga group[J]. Cir Esp, 2006; 79(2)∶101.
2.	David AI, Aquino CG, Guidoni LR, et al. Experimental training model of pancreas transplant[J]. Transplant Proc, 2006; 38(6)∶1941.
3.	Ito T, Shimada K, Gang M, et al. Pancreas transplantation using type Ⅰ and type Ⅱ spontaneously diabetic rats——Our experimental experience[J]. Microsurgery, 2007; 27(4)∶305.
4.	Maglione M, Hermann M, Hengster P, et al. A novel technique for heterotopic vascularized pancreas transplantation in mice to assess ischemia reperfusion injury and graft pancreatitis[J]. Surgery, 2007; 141(5)∶682.
5.	Liu X, Drognitz O, Neeff H, et al. Apoptosis is caused by prolonged organ preservation and blocked by apoptosis inhibitor in experimental rat pancreatic grafts[J]. Transplant Proc， 2004; 36(4)∶1209.
6.	Drognitz O, Michel P, Koczan D, et al. Characterization of ischemia/reperfusion-induced gene expression in experimental pancreas transplantation[J]. Transplantation, 2006; 81(10)　∶1428.
7.	Drognitz O, Obermaier R, Liu X, et al. Effects of organ preservation, ischemia time and caspase inhibition on apoptosis and microcirculation in rat pancreas transplantation[J]. Am J Transplant, 2004; 4(7)∶1042.
8.	Drognitz O, Liu X, Obermaier R, et al. Ischemic preconditioning fails to improve microcirculation but increases apoptotic cell death in experimental pancreas transplantation[J]. Transpl Int， 2004; 17(6)∶317.
9.	Munoz-Casares FC, Padillo FJ, Briceno J, et al. Melatonin reduces apoptosis and necrosis induced by ischemia/reperfusion injury of the pancreas[J]. J Pineal Res, 2006; 40(3)∶195.
10.	Lee S, Tung KS, Koopmans H, et al. Pancreaticoduodenal transplantation in the rat[J]. Transplantation， 1972; 13(4)　∶421.
11.	刁畅，程若川，魏晓岗，等. 大鼠胰十二指肠移植缺血再灌注损伤模型的建立[J]. 中华实验外科杂志， 2003； 20（10）　∶944.
12.	Cervera C, Moreno A. Infections in recipients of a kidney-pancreas transplant[J]. Enferm Infecc Microbiol Clin， 2007; 25(5)∶345.
13.	Berger N, Guggenbichler S, Steurer W, et al. Related Articles, Links Bloodstream infection following 217 consecutive systemic-enteric drained pancreas transplants[J]. BMC Infect Dis, 2006; 6（1）∶127.
14.	Adeghate E. Effect of subcutaneous pancreatic tissue transpl-ants on streptozotocin-induced diabetes in rats. Ⅰ. Morphological studies on normal, diabetic and transplanted pancreatic tissues[J]. Tissue Cell， 1999; 31(1)∶66.
15.	van de Linde P, van der Boog PJ, Baranski AG, et al. Pancreas transplantation: advantages of both enteric and bladder drainage combined in a two-step approach[J]. Clin Transplant， 2006; 20(2)∶253.
16.	Rangel EB, Gonzalez AM, Linhares MM, et al. Thrombotic microangiopathy after simultaneous pancreas-kidney transplantation[J]. Clin Transplant， 2007; 21(2)∶241.
17.	Maglione M, Hermann M, Hengster P, et al. Tetrahydrobiopterin attenuates microvascular reperfusion injury following murine pancreas transplantation[J]. Am J Transplant， 2006; 6(7)∶1551.
18.	Preissler G, Massberg S, Waldner H, et al. Intermittent capillary perfusion in rat pancreas grafts following short- and long-term preservation in University of Wisconsin solution[J]. Transpl Int， 2006; 19(4)∶325.
19.	王杰，顾永平. 大鼠胰十二指肠移植动物模型的显微外科技术改进[J]. 东南大学学报（医学版）， 2002； 21（2）∶153.
20.	倪晓光，关凤林，刘忠，等. 大鼠全胰十二指肠移植术并发症的分析及预防[J]. 大连医科大学学报， 2001； 23（1）∶43.

1. 　Navarro-Pinero A, Castro-Santiago MJ, Aranda-Narvaez JM, et al. Pancreatic transplantation. Results of the Malaga group[J]. Cir Esp, 2006; 79(2)∶101.
2. 　David AI, Aquino CG, Guidoni LR, et al. Experimental training model of pancreas transplant[J]. Transplant Proc, 2006; 38(6)∶1941.
3. 　Ito T, Shimada K, Gang M, et al. Pancreas transplantation using type Ⅰ and type Ⅱ spontaneously diabetic rats——Our experimental experience[J]. Microsurgery, 2007; 27(4)∶305.
4. 　Maglione M, Hermann M, Hengster P, et al. A novel technique for heterotopic vascularized pancreas transplantation in mice to assess ischemia reperfusion injury and graft pancreatitis[J]. Surgery, 2007; 141(5)∶682.
5. 　Liu X, Drognitz O, Neeff H, et al. Apoptosis is caused by prolonged organ preservation and blocked by apoptosis inhibitor in experimental rat pancreatic grafts[J]. Transplant Proc， 2004; 36(4)∶1209.
6. 　Drognitz O, Michel P, Koczan D, et al. Characterization of ischemia/reperfusion-induced gene expression in experimental pancreas transplantation[J]. Transplantation, 2006; 81(10)　∶1428.
7. 　Drognitz O, Obermaier R, Liu X, et al. Effects of organ preservation, ischemia time and caspase inhibition on apoptosis and microcirculation in rat pancreas transplantation[J]. Am J Transplant, 2004; 4(7)∶1042.
8. 　Drognitz O, Liu X, Obermaier R, et al. Ischemic preconditioning fails to improve microcirculation but increases apoptotic cell death in experimental pancreas transplantation[J]. Transpl Int， 2004; 17(6)∶317.
9. 　Munoz-Casares FC, Padillo FJ, Briceno J, et al. Melatonin reduces apoptosis and necrosis induced by ischemia/reperfusion injury of the pancreas[J]. J Pineal Res, 2006; 40(3)∶195.
10. 　Lee S, Tung KS, Koopmans H, et al. Pancreaticoduodenal transplantation in the rat[J]. Transplantation， 1972; 13(4)　∶421.
11. 　刁畅，程若川，魏晓岗，等. 大鼠胰十二指肠移植缺血再灌注损伤模型的建立[J]. 中华实验外科杂志， 2003； 20（10）　∶944.
12. 　Cervera C, Moreno A. Infections in recipients of a kidney-pancreas transplant[J]. Enferm Infecc Microbiol Clin， 2007; 25(5)∶345.
13. 　Berger N, Guggenbichler S, Steurer W, et al. Related Articles, Links Bloodstream infection following 217 consecutive systemic-enteric drained pancreas transplants[J]. BMC Infect Dis, 2006; 6（1）∶127.
14. 　Adeghate E. Effect of subcutaneous pancreatic tissue transpl-ants on streptozotocin-induced diabetes in rats. Ⅰ. Morphological studies on normal, diabetic and transplanted pancreatic tissues[J]. Tissue Cell， 1999; 31(1)∶66.
15. 　van de Linde P, van der Boog PJ, Baranski AG, et al. Pancreas transplantation: advantages of both enteric and bladder drainage combined in a two-step approach[J]. Clin Transplant， 2006; 20(2)∶253.
16. 　Rangel EB, Gonzalez AM, Linhares MM, et al. Thrombotic microangiopathy after simultaneous pancreas-kidney transplantation[J]. Clin Transplant， 2007; 21(2)∶241.
17. 　Maglione M, Hermann M, Hengster P, et al. Tetrahydrobiopterin attenuates microvascular reperfusion injury following murine pancreas transplantation[J]. Am J Transplant， 2006; 6(7)∶1551.
18. 　Preissler G, Massberg S, Waldner H, et al. Intermittent capillary perfusion in rat pancreas grafts following short- and long-term preservation in University of Wisconsin solution[J]. Transpl Int， 2006; 19(4)∶325.
19. 　王杰，顾永平. 大鼠胰十二指肠移植动物模型的显微外科技术改进[J]. 东南大学学报（医学版）， 2002； 21（2）∶153.
20. 　倪晓光，关凤林，刘忠，等. 大鼠全胰十二指肠移植术并发症的分析及预防[J]. 大连医科大学学报， 2001； 23（1）∶43.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Establishment of Pancreas-Duodenal Transplantation Model in Rat with Dual Cuff Technique

Abstract Full text Figures/Tables Video References Cited by

Format

Content