Experimental Study on Differentiation of Human Cord Blood Stem Cells into Hepatocytes_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

HUANG Tao ¹ , LI Bo ¹ , LIN Haoming ¹ , QIN Yang ²

Department of General Surgery, West China Hospital， Sichuan University， Chengdu 610041， China;

Corresponding author：

Keywords：

Human umbilical cord blood; Stem cell; Hepatocyte; Differentiation

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Objective 　To investigate the feasibility of differentiating human umbilical cord blood stem cells into hepatocytes.
Methods 　Thirty-six BALB/c nude mice were randomly divided into experimental group and control group(18 in each of the group), and experimental group was again randomly divided into group A， B and C (six in each of the group). The mice in experimental group and control group were exposed to 350 cGy radiation produced by 60Ｃｏ. After 3 h, karyocytes at different concentrations in the fresh human umbilical cord blood were injected into the mice in experimental group A, B, C via their tail veins, and the equal volume of normal sodium (NS) was also injected into control group via tail veins. After one month, carbon tetrachloride (CCl4) was injected into experimental group A, B and control group via abdominal cavity, and the equal volume of normal sodium was injected into experimental group C. After two months, immunohistochemistry and reverse transcriptase polymerase chain reaction (RT-PCR) were used to detect the expressions of human cytokeratin-18 (CK18), cytokeratin-19 (CK19) and albumin (ALB) in liver tissues of all mice.
Results 　The expressions of CK18, CK19 and ALB in injured liver tissues were all positive, and the expressions of experimental group B were higher than those of experimental group A （P＜0.05）, but the expressions of CK18, CK19 and ALB in the liver tissues of control group and experimental group C, whose were not injured with CCl4, were all negative.
Conclusion 　Human umbilical cord blood-derived stem cells can differentiate into hepatocytes and express ALB under special microenvironment after liver injured by CCl4　, and the expression level of ALB maybe directly related to the number of human umbilical cord blood stem cells.

Citation： HUANG Tao,LI Bo,LIN Haoming,QIN Yang. Experimental Study on Differentiation of Human Cord Blood Stem Cells into Hepatocytes. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2007, 14(4): 423-427. doi: Copy

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13.	Lowes KN, Brennan BA, Yeoh GC, et al. Oval cell numbers in human chronic liver diseases are directly related to disease severity [Ｊ]　. Am J Pathol， 1999; 154(2)∶537.
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1. 　Petersen BE， Bowen WC， Patrene KD， et al. Bone marrow as a potential source of hepatic oval cells [Ｊ]　. Science， 1999； 284(5417)∶1168.
2. 　Wang X, Ge S, McNamara G, et al. Albumin-expressing hepatocyte-like cells develop in the livers of immune-deficient mice that received transplants of highly purified human hematopoietic stem cells [Ｊ]　. Blood, 2003; 101(10)∶4201.
3. 　Ying QL, Nichols J, Evans EP, et al. Changing potency by spontaneous fusion [Ｊ]　. Nature， 2002; 416(6880)∶545.
4. 　Lee OK, Kuo TK, Chen WM, et al. Isolation of multipotent mesenchymal stem cells from umbilical cord blood [Ｊ]　. Blood， 2004; 103(5)∶1669.
5. 　Fiegel HC, Lioznov MV, Cortes-Dericks L, et al. Liver-specific gene expression in cultured human hematopoietic stem cells [Ｊ]　. Stem Cells， 2003; 21(1)∶98.
6. 　Terstappen LW, Huang S, Safford M, et al. Sequential generations of hematopoietic colonies derived from single nonlineage-committed CD34+CD38- progenitor cells [Ｊ]　. Blood， 1991; 77(6)∶1218.
7. 　丁顺利，诸建新，赵钧铭，等. 骨健移植造血重建过程中干/祖细胞增殖特性的研究 [Ｊ]　. 中国实验血液学杂志， 1999； 7(2)∶138.
8. 　王嵋景，杨崇礼，符仁义，等. 人脐血干细胞对裸小鼠移植的实验研究 [Ｊ]　. 四川医学， 2002； 23(8)∶831.
9. 　Hogan CJ, Shpall EJ, McNulty O, et al. Engraftment and development of human CD34+-enriched cells from umbilical cord blood in NOD/LtSz-scid/scid mice [Ｊ]　. Blood, 1997; 90(1)∶85.
10. 　金世龙，刘宝华，刘宏鸣，等. 大鼠肝卵圆细胞的分离培养及干细胞标志分析 [Ｊ]　. 中国普外基础与临床杂志, 2007; 14(1)∶68　.
11. 　马军，段芳龄，颜伏归，等. 部分肝切除后的肝损伤血清和肝细胞生长因子促进大鼠骨髓细胞表达甲胎蛋白和白蛋白 [Ｊ]　. 中华肝脏病杂志， 2004; 12（7）∶410.
12. 　Theise ND, Saxena R, Portmann BC, et al. The canals of Hering and hepatic stem cells in humans [Ｊ]　. Hepatology， 1999; 30(6)　∶1425.
13. 　Lowes KN, Brennan BA, Yeoh GC, et al. Oval cell numbers in human chronic liver diseases are directly related to disease severity [Ｊ]　. Am J Pathol， 1999; 154(2)∶537.
14. 　Schmidt C, Bladt F, Goedecke S, et al. Scatter factor/hepatocyte growth factor is essential for liver development [Ｊ]　. Nature， 1995; 373(6516)∶699.
15. 　French SW, Hoyer KK, Shen RR, et al. Transdifferentiation and nuclear reprogramming in hematopoietic development and neoplasia [Ｊ]　. Immunol Rev, 2002; 187∶22.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Experimental Study on Differentiation of Human Cord Blood Stem Cells into Hepatocytes

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