Investigation on Perioperative Renal Aquaporin 2 Expression in Experimental Obstructive Jaundice_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

WANG Yong , HAN Jilong , LIU Jingang

Department of The Second Biliary Surgery, Shengjing Hospital, China Medical University, Shenyang 110004, China;

Corresponding author：

Keywords：

Obstructive jaundice; Aquaporin 2; Perioperative period

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Objective 　To investigate the changes of renal medulla aquaporin 2 expression and morphological changes of epithelia of collecting tube after bile duct recanalizaiton operation.
Methods 　Thirty rats were divided into two groups randomly. Common bile duct ligation was performed on 20 experimental rats with silicon tubes 2 mm in extre-diameter, and sham operation on the other 10 rats. Seven days later, bile duct recanalizaiton was performed on obstructive jaundice group and sham operation on contrast group. Experimental rats were divided into two subgroups randomly. Half of them were killed immediately and the others would be killed 24 hours later. Serum of each rat was collected to detect hepatic function and renal function. Renal medulla was fixed for microscopic examination and was kept in the -80 ℃ refrigerator for aquaporin 2 expression measurement by Western blot technique.
Results 　All of the animals accomplished the experiment smoothly. Golden ascites were found in the rats of obstructive jaundice group. Twenty-four hours after recanalization, serum bilirubin levels decreased 〔(45.95±8.39) μmol/L〕, P＜0.01, and there was no significant change in blood urine and creatine level. Compared with sham operation group (21 966.20±1 544.70), expression of aquaporin 2 decreased significantly after common bile duct ligation in obstructive jaundice group (15 665.30±1 181.85), P＜0.01. After recanalizaion, the expression of aquaporin 2 in obstructive jaundice group increased (19 490.80±4 239.32), P＜0.01.
Conclusion 　Common bile duct obstruction would lead to epithelium injury of renal collecting tube, and down regulate the aquaporin 2 expression.

Citation： WANG Yong,HAN Jilong,LIU Jingang. Investigation on Perioperative Renal Aquaporin 2 Expression in Experimental Obstructive Jaundice. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2007, 14(5): 538-542. doi: Copy

1.	Fogarty BJ, Parks RW, Rowlands BJ, et al. Renal dysfunction in obstructive jaundice [J]. Br J Surg， 1995; 82(7)∶877.
2.	Preston GM, Carroll TP, Guggino WB, et al. Appearance of water channels in xenopus oocytes expressing red cell CHIP28 protein [J]. Science, 1992; 256(5055)∶385.
3.	King LS, Kozono D, Agre P. From structure to disease: the evolving tale of aquaporin biology [J]. Nat Rev Mol Cell Biol， 2004; 5(9)∶687.
4.	Sohn EJ, Kang DG, Lee HS. Protective effects of glycyrrhizin on gentamicin-induced acute renal failure in rats [J]. Pharmacol Toxicol， 2003; 93(3)∶116.
5.	Inan M, Sayek I, Tel BC, et al. Role of endotoxin and nitric oxide in the pathogenesis of renal failure in obstructive jaundice [J]. Br J Surg， 1997; 84(7)∶943.
6.	王勇，刘金钢，宋军，等. 生长激素对大鼠梗阻性黄疸肠菌移位的影响 [J]. 中华实验外科杂志， 2002; 19(1)∶85.
7.	刘金钢，王勇，宋军，等. 生长激素对实验性梗阻性黄疸大鼠内毒素血症的防治 [J]. 中华普通外科杂志， 2001; 16(8)∶493.
8.	刘金钢，那延立，郭宏斌，等. 生长激素减轻梗阻性黄疸时肠菌及内毒素移位的实验研究 [J]. 中国普外基础与临床杂志， 2002; 9(3)∶172.
9.	刘金钢，宋军，田野，等. 生长激素对梗阻性黄疸时肿瘤坏死因子-α及内毒素血症的影响 [J]. 中华实验外科杂志， 2002; 19(5)∶473.
10.	Zeidel ML, Ambudkar SV, Smith BL, et al. Reconstitution of functional water channels in liposomes containing purified red cell CHIP28 protein [J]. Biochemistry， 1992; 31(33)∶7436.
11.	Nielsen S, Frokiaer J, Marples D, et al. Aquaporins in the kidney: from molecules to medicine [J]. Physiol Rev， 2002; 82(1)∶205.
12.	Gong H, Wang W, Kwon TH, et al. Reduced renal expression of AQP2, p-AQP2 and AQP3 in haemorrhagic shock-induced acute renal failure [J]. Nephrol Dial Transplant， 2003; 18(12)∶2551.
13.	Nejsum LN. The renal plumbing system: aquaporin water ch-annels [J]. Cell Mol Life Sci， 2005; 62(15)∶1692.
14.	Jung JS, Lee RH, Koh SH, et al. Changes in expression of sodium cotransporters and aquaporin-2 during ischemia-reperfusion injury in rabbit kidney [J]. Ren Fail， 2000; 22(4)∶407.
15.	Kim SW, Jeon YS, Lee JU, et al. Diminished adenylate cyclase activity and aquaporin 2 expression in acute renal failure rats [J]. Kidney Int， 2000; 57(4)∶1643.
16.	Lee S, Kim W, Moon SO, et al. Rosiglitazone ameliorates cisplatin-induced renal injury in mice [J]. Nephrol Dial Transplant， 2006; 21(8)∶2096.
17.	Misharin AV, Resnenko AB, Fidelina OV, et al. Antidiuretic hormone-V2-receptor-aquaporin-2 system in rat kidneys during acute inflammation [J]. Bull Exp Biol Med， 2004; 138(5)∶452.
18.	Cauwels A. Nitric oxide in shock [J]. Kidney Int， 2007 May 30.
19.	Padillo FJ, Cruz A, Briceno J, et al. Multivariate analysis of factors associated with renal dysfunction in patients with obstructive jaundice [J]. Br J Surg， 2005; 92(11)∶1388.
20.	Uslu A, Cayci M, Nart A, et al. Renal failure in obstructive jaundice [J]. Hepatogastroenterology， 2005; 52(61)∶52.

1. 　Fogarty BJ, Parks RW, Rowlands BJ, et al. Renal dysfunction in obstructive jaundice [J]. Br J Surg， 1995; 82(7)∶877.
2. 　Preston GM, Carroll TP, Guggino WB, et al. Appearance of water channels in xenopus oocytes expressing red cell CHIP28 protein [J]. Science, 1992; 256(5055)∶385.
3. 　King LS, Kozono D, Agre P. From structure to disease: the evolving tale of aquaporin biology [J]. Nat Rev Mol Cell Biol， 2004; 5(9)∶687.
4. 　Sohn EJ, Kang DG, Lee HS. Protective effects of glycyrrhizin on gentamicin-induced acute renal failure in rats [J]. Pharmacol Toxicol， 2003; 93(3)∶116.
5. 　Inan M, Sayek I, Tel BC, et al. Role of endotoxin and nitric oxide in the pathogenesis of renal failure in obstructive jaundice [J]. Br J Surg， 1997; 84(7)∶943.
6. 　王勇，刘金钢，宋军，等. 生长激素对大鼠梗阻性黄疸肠菌移位的影响 [J]. 中华实验外科杂志， 2002; 19(1)∶85.
7. 　刘金钢，王勇，宋军，等. 生长激素对实验性梗阻性黄疸大鼠内毒素血症的防治 [J]. 中华普通外科杂志， 2001; 16(8)∶493.
8. 　刘金钢，那延立，郭宏斌，等. 生长激素减轻梗阻性黄疸时肠菌及内毒素移位的实验研究 [J]. 中国普外基础与临床杂志， 2002; 9(3)∶172.
9. 　刘金钢，宋军，田野，等. 生长激素对梗阻性黄疸时肿瘤坏死因子-α及内毒素血症的影响 [J]. 中华实验外科杂志， 2002; 19(5)∶473.
10. 　Zeidel ML, Ambudkar SV, Smith BL, et al. Reconstitution of functional water channels in liposomes containing purified red cell CHIP28 protein [J]. Biochemistry， 1992; 31(33)∶7436.
11. 　Nielsen S, Frokiaer J, Marples D, et al. Aquaporins in the kidney: from molecules to medicine [J]. Physiol Rev， 2002; 82(1)∶205.
12. 　Gong H, Wang W, Kwon TH, et al. Reduced renal expression of AQP2, p-AQP2 and AQP3 in haemorrhagic shock-induced acute renal failure [J]. Nephrol Dial Transplant， 2003; 18(12)∶2551.
13. 　Nejsum LN. The renal plumbing system: aquaporin water ch-annels [J]. Cell Mol Life Sci， 2005; 62(15)∶1692.
14. 　Jung JS, Lee RH, Koh SH, et al. Changes in expression of sodium cotransporters and aquaporin-2 during ischemia-reperfusion injury in rabbit kidney [J]. Ren Fail， 2000; 22(4)∶407.
15. 　Kim SW, Jeon YS, Lee JU, et al. Diminished adenylate cyclase activity and aquaporin 2 expression in acute renal failure rats [J]. Kidney Int， 2000; 57(4)∶1643.
16. 　Lee S, Kim W, Moon SO, et al. Rosiglitazone ameliorates cisplatin-induced renal injury in mice [J]. Nephrol Dial Transplant， 2006; 21(8)∶2096.
17. 　Misharin AV, Resnenko AB, Fidelina OV, et al. Antidiuretic hormone-V2-receptor-aquaporin-2 system in rat kidneys during acute inflammation [J]. Bull Exp Biol Med， 2004; 138(5)∶452.
18. 　Cauwels A. Nitric oxide in shock [J]. Kidney Int， 2007 May 30.
19. 　Padillo FJ, Cruz A, Briceno J, et al. Multivariate analysis of factors associated with renal dysfunction in patients with obstructive jaundice [J]. Br J Surg， 2005; 92(11)∶1388.
20. 　Uslu A, Cayci M, Nart A, et al. Renal failure in obstructive jaundice [J]. Hepatogastroenterology， 2005; 52(61)∶52.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Investigation on Perioperative Renal Aquaporin 2 Expression in Experimental Obstructive Jaundice

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