Effect of Tumor Suppressor RECK Gene on Biological Behavior of Hepatocellular Carcinoma_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

ZHANG Yong , ZHENG Qichang , LU Xin , QIN Tao.

Department of General Surgery, Xiehe Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China;

Corresponding author：

Keywords：

Reversion-inducing-cysteine-rich protein with Kazal motifs gene HepG2 cell Transfect

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【Abstract】ObjectiveTo explore the effects of RECK gene on the biological behaviors of hepatocellular (HepG2).
MethodsThe RECK cDNA was transfected to HepG2 with lipofectamine 2000. Detected its protein expressions with Western blot before and after transfection, analyzed the effects of RECK on MMP-9 activity using gelatin zymography, observed the effects on proliferation ability by MTT assay and plate clone formation assay, compare the changes of invasion ability by cell adhesion assay and in vitro invasion experiment.
Results RECK protein was expressed steadily in transfected HepG2 cells and the amount of activated MMP-9 were decreased significantly. Their proliferation abilities weren’t different before and after transfection but their invasion abilities decreased sharply.
ConclusionRECK gene can transfect HepG2 cells by liposome efficiently. It can inhibit the activity of MMP-9 and the invasion ability of HepG2.

Citation： ZHANG Yong,ZHENG Qichang,LU Xin,QIN Tao.. Effect of Tumor Suppressor RECK Gene on Biological Behavior of Hepatocellular Carcinoma. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2006, 13(2): 150-153. doi: Copy

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3.	Eisenberg I, Hochner H, Sadeh M, et al. Establishment of the genomic structure and identification of thirteen singlenucleotide polymorphisms in the human RECK gene [Ｊ]. Cytogenet Genome Res, 2002; 97(1-2)∶58.
4.	Sasahara RM, Takahashi C, Noda M. Involvement of the Sp1 site in rasmediated downregulation of the RECK metastasis suppressor gene [Ｊ]. Biochem Biophys Res Commun, 1999; 264(3)∶668.
5.	Oh J, Takahashi R, Kondo S, et al. The membraneanchored MMP inhibitor RECK is a key regulator of extracellular matrix integrity and angiogenesis [Ｊ]. Cell, 2001; 107(6)∶789.
6.	Span PN, Sweep CG, Manders P, et al. Matrix metalloproteinase inhibitor reversioninducing cysteinerich protein with Kazal motifs: a prognostic marker for good clinical outcome in human breast carcinoma [Ｊ]. Cancer, 2003; 97(11)∶2710.
7.	Masui T, Doi R, Koshiba T, et al. RECK expression in pancreatic cancer: its correlation with lower invasiveness and better prognosis [Ｊ]. Clin Cancer Res, 2003; 9(5)∶1779.
8.	Furumoto K, Arii S, Mori A, et al. RECK gene expression in hepatocellular carcinoma: correlation with invasionrelated clinicopathological factors and its clinical significance. Reverseinducingcysteinerich protein with Kazal motifs [Ｊ]. Hepatology, 2001; 33(1)∶189.

1. Takahashi C, Sheng Z, Horan TP, et al. Regulation of matrix metalloproteinase9 and inhibition of tumor invasion by the membraneanchored glycoprotein RECK [Ｊ]. Proc Natl Acad Sci USA, 1998; 95(22)∶ 13221.
2. 骞爱荣，商澎，陈志南. 改良明胶酶谱测定明胶酶活性 [Ｊ]. 解放军医学杂志， 2003； 28（3）∶282.
3. Eisenberg I, Hochner H, Sadeh M, et al. Establishment of the genomic structure and identification of thirteen singlenucleotide polymorphisms in the human RECK gene [Ｊ]. Cytogenet Genome Res, 2002; 97(1-2)∶58.
4. Sasahara RM, Takahashi C, Noda M. Involvement of the Sp1 site in rasmediated downregulation of the RECK metastasis suppressor gene [Ｊ]. Biochem Biophys Res Commun, 1999; 264(3)∶668.
5. Oh J, Takahashi R, Kondo S, et al. The membraneanchored MMP inhibitor RECK is a key regulator of extracellular matrix integrity and angiogenesis [Ｊ]. Cell, 2001; 107(6)∶789.
6. Span PN, Sweep CG, Manders P, et al. Matrix metalloproteinase inhibitor reversioninducing cysteinerich protein with Kazal motifs: a prognostic marker for good clinical outcome in human breast carcinoma [Ｊ]. Cancer, 2003; 97(11)∶2710.
7. Masui T, Doi R, Koshiba T, et al. RECK expression in pancreatic cancer: its correlation with lower invasiveness and better prognosis [Ｊ]. Clin Cancer Res, 2003; 9(5)∶1779.
8. Furumoto K, Arii S, Mori A, et al. RECK gene expression in hepatocellular carcinoma: correlation with invasionrelated clinicopathological factors and its clinical significance. Reverseinducingcysteinerich protein with Kazal motifs [Ｊ]. Hepatology, 2001; 33(1)∶189.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effect of Tumor Suppressor RECK Gene on Biological Behavior of Hepatocellular Carcinoma

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