Study of E-cadherin Antisense Oligodeoxynucleotide on Invasive Ability of Tumor Cells_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

CHE Xiangming , WANG Shufeng , FAN Lin , ZHANG Ruyuan , ZHAO Wei.

Department of General Surgery, The First Hospital, Xi’an Jiaotong University, Xi’an 710061, China;

Corresponding author：

Keywords：

E-cadherin Tumor cell Invasion Antisense oligodeoxynucleotied

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【Abstract】ObjectiveTo study the effect of down-regulation of E-cadherin on the invasion ability of tumor cells.
MethodsHuman pancreatic carcinoma cell line JHP-1 was treated with E-cadherin antisense oligodeoxynucleotied (ASODN). The immunocytochemistry, Western blot were used to detect the expression and the contents of E-cadherin in the tumor cells, and the invasive ability of tumor cells were evaluated by invasive-MTT assay.
Results Treated with E-cadherin ASODN, the expression of E-cadherin on JHP-1 cells were reduced, and the protein contents were decreased as well compared with control groups and ODN group. The invasive ability of JHP-1 cells to the basement membrane was increased (P＜0.001) compared with ODN group and control group.
ConclusionE-cadherin was related to the invasive ability of tumor cells.

Citation： CHE Xiangming,WANG Shufeng,FAN Lin,ZHANG Ruyuan,ZHAO Wei.. Study of E-cadherin Antisense Oligodeoxynucleotide on Invasive Ability of Tumor Cells. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2006, 13(2): 191-193. doi: Copy

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1. Xiangming C, Hokita S, Nuruki K, et al. The expression of cadherincatenin complex in association with the clinicopathologic features of early gastric cancer [Ｊ]. Surg Today, 1998; 28(6)∶ 587.
2. 董全进，邹寿椿，邓高里，等. 表皮钙黏附素和α连接素在结直肠癌中表达的临床意义 [Ｊ]. 中华胃肠外科杂志， 2002; 5（4）∶291.
3. Xiangming C, Hokita S, Natsugoe S, et al. Cooccurrence of reduced expression of alphacatenin and overexpression of p53 is a predictor of lymph node metastasis in early gastric cancer [Ｊ]. Oncology, 1999; 57(2)∶131.
4. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of proteindye binding [Ｊ]. Anal Biochem, 1976; 72（1）∶248.
5. Jansen B, SchlagbauerWadl H, Brown BD, et al. bcl2 antisense therapy chemosensitizes human melanoma in SCID mice [Ｊ]. Nat Med, 1998; 4(2)∶232.
6. Imamura H, Takao S, Aikou T. A modified invasion3(4,5dimethylthiazole2yl)2,5diphenyltetrazolium bromide assay for quantitating tumor cell invasion [Ｊ]. Cancer Res, 1994; 54(13)∶3620.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Study of E-cadherin Antisense Oligodeoxynucleotide on Invasive Ability of Tumor Cells

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