Alterations of Toll Like Receptor 4 on Peripheral Blood Monocytes During Early Stage of Human Acute Pancreatitis_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

LI Hongguang ¹ , ZHOU Zongguang ¹ , LEI Song ² , ZHU Lin ³ , ZHENG Xuelian ¹ , ZHANG Hongyan ³ , WANG Yong ¹ , ZHANGJiancheng. ¹

Institute of Digestive Surgery, West China Hospital, Sichuan University, Chengdu 610041, China;

Corresponding author：

Keywords：

Acute pancreatitis Heparan sulfate Toll like receptor 4 Innate immune

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【Abstract】ObjectiveTo study Toll like receptor 4 (TLR4) expression on peripheral blood monocytes (PBMCs) during the early stage of human acute pancreatitis.
MethodsThirty consecutive patients with acute pancreatitis admitted within 24 h of onset of abdominal pain were enrolled in this study. Another 20 healthy volunteers were included as control. Blood samples were collected by venipuncture on the day of admission and 3， 7 d after admission and PBMCs were isolated. TLR4 and CD14 expressions on PBMCs were detected by flow cytometry. Serum tumor necrosis factor alpha (TNF-α) and interleukin 6 (IL-6) were measured simultaneously. Correlations between these parameters were analyzed.
ResultsTLR4 expression increased on the day of admission and then continued to decline for several days. On third day, TLR4 expression was almost normal compared with the normal control. The alteration of serum TNF-α was consistent with that of TLR4.
ConclusionDuring the early stage of human acute pancreatitis, mononuclear-macrophages may be ignited through TLR4 (a door keeper of innate immune system), which lead to TNF-α production.

Citation： LI Hongguang,ZHOU Zongguang,LEI Song,ZHU Lin,ZHENG Xuelian,ZHANG Hongyan,WANG Yong,ZHANGJiancheng.. Alterations of Toll Like Receptor 4 on Peripheral Blood Monocytes During Early Stage of Human Acute Pancreatitis. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2006, 13(2): 197-200. doi: Copy

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1. [Ｊ].Science, 2002; 296(5566)∶ 301.
2.
3.
4.
5. [Ｊ]. J Immunol， 2004; 172(1)∶20.
6.
7. [Ｊ]. Arch Surg, 1993; 128(5)∶586.
8.
9. [Ｊ]. J Surg Res, 1996; 63(1)∶369.
10. [Ｊ]. Am J Respir Crit Care Med, 2003; 168(2)∶148.
11. [Ｊ].Dig Dis Sci, 1997; 42(8)∶1783.
12.
13. [Ｊ].Gastroenterology, 1997; 113(5)∶1741.
14. [Ｊ]. Surgery, 2004; 135(4)∶376.
15. [Ｊ]. Circulation, 2002; 105(6)∶685.
16. [Ｊ]. Pancreas, 2005; 30(4)∶375.
17. [Ｊ]. Eur J Surg, 2000; 166(8)∶628.
18. Matzinger P. The danger model: a renewed sense of self.
19. Jaffray C, Yang J, Norman J. Elastase mimics pancreatitisinduced hepatic injury via inflammatory mediators [Ｊ]. J Surg Res, 2000; 90(2)∶95.
20. Murr MM, Yang J, Fier A, et al. Pancreatic elastase induces liver injury by activating cytokine production within Kupffer cells via nuclear factorKappa B [Ｊ]. J Gastrointest Surg, 2002; 6(3)∶474.
21. Johnson GB, Brunn GJ, Platt JL. Cutting edge: an endogenous pathway to systemic inflammatory response syndrome (SIRS)like reactions through Tolllike receptor 4.
22. Bradley EL 3rd. A clinically based classification system for acute pancreatitis. Summary of the International Symposium on Acute Pancreatitis, Atlanta, Ga, September 11 through 13, 1992.
23. Fink GW, Norman JG. Intrapancreatic interleukin1beta gene expression by specific leukocyte populations during acute pancreatitis.
24. Dugernier TL, Laterre PF, Wittebole X, et al. Compartmentalization of the inflammatory response during acute pancreatitis: correlation with local and systemic complications.
25. Norman JG, Fink GW, Denham W, et al. Tissuespecific cytokine production during experimental acute pancreatitis. A probable mechanism for distant organ dysfunction.
26. Denham W, Yang J, Fink G, et al. Gene targeting demonstrates additive detrimental effects of interleukin 1 and tumor necrosis factor during pancreatitis.
27. Ikushima H, Nishida T, Takeda K, et al. Expression of Tolllike receptors 2 and 4 is downregulated after operation.
28. Dybdahl B, Wahba A, Lien E, et al. Inflammatory response after open heart surgery: release of heatshock protein 70 and signaling through tolllike receptor4.
29. Li Y, Zhou ZG, Xia QJ, et al. Tolllike receptor 4 detected in exocrine pancreas and the change of expression in ceruleininduced pancreatitis.
30. Gotzinger P, Sautner T, Spittler A, et al. Severe acute pancreatitis causes alterations in HLADR and CD14 expression on peripheral blood monocytes independently of surgical treatment.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Alterations of Toll Like Receptor 4 on Peripheral Blood Monocytes During Early Stage of Human Acute Pancreatitis

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