Study on Gallbladder Carcinoma Apoptosis Induced by Antisense Oligodeoxynucleotide Targeting survivin_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

FENG Limin , JIANG Xihong , WU Xinlin , WANG Jianli , SHOU Nanhai.

Department of Surgery, Qilu Hospital, Shandong University, Jinan 250012, China;

Corresponding author：

Keywords：

Antisense oligodeoxynucleotide survivin Apoptosis Gallbladder carcinoma

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【Abstract】ObjectiveTo study the apoptosis of gallbladder carcinoma cell line GBCSD induced by antisense oligodeoxynucleotide (ASODN) targeting survivin.
MethodsASODN targeting survivin was transfected into GBCSD cells mediated by lipofectin. Cultured cells were divided into 3 groups: control group,sense oligonucleotide (SODN) group and ASODN group. After transfected for 16 h, the cultured cells were harvested and the following texts were carried out. The expression of survivin mRNA was detected by RTPCR. Flow cytometer were used to detect apoptosis. Morphological changes were observed by electron microscopy.
ResultsThe expression of survivin mRNA was decreased 47.83% in ASODN group while apoptosis was increased from (0.50±0.23)% to (26.28±3.91)%. Abnormal morphological changes of cells were observed in ASODN group and apoptosis bodies were found in some gallbladder carcinoma cells.
ConclusionThe expression of survivin may be decreased in GBCSD cells after ASODN transfection.ASODN targeting survivin could induce gallbladder carcinoma cells apoptosis effectively.

Citation： FENG Limin,JIANG Xihong,WU Xinlin,WANG Jianli,SHOU Nanhai.. Study on Gallbladder Carcinoma Apoptosis Induced by Antisense Oligodeoxynucleotide Targeting survivin. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2006, 13(3): 298-301. doi: Copy

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1. Adida C, Crotty PL, McGrath J, et al. Developmentally regulated expression of the novel cancer antiapoptosis gene survivin in human and mouse differentiation [Ｊ]. Am J Pathol, 1998; 152(1)∶ 43.
2. Todoroki T, Kawamoto T, Takahashi H, et al. Treatment of gallbladder cancer by radical resection [Ｊ]. Br J Surg, 1999; 86(5)∶622.
3. Hay BA, Wassarman DA, Rubin GM. Drosophila homologs of baculovirus inhibitor of apoptosis proteins function to block cell death [Ｊ]. Cell, 1995; 83(7)∶1253.
4. ZangemeisterWittke U, Simon HU. An IAP in action: the multiple roles of survivin in differentiation, immunity and malignancy [Ｊ]. Cell Cycle, 2004; 3(9)∶1121.
5. Li F, Ambrosini G, Chu EY, et al. Control of apoptosis and mitotic spindle checkpoint by survivin [Ｊ]. Nature, 1998; 396(6711)∶580.
6. Sarela AI, Macadam RC, Farmery SM, et al. Expression of the antiapoptosis gene, survivin, predicts death from recurrent colorectal carcinoma [Ｊ]. Gut, 2000; 46(5)∶645.
7. 王治, 梅柱中, 童新，等. 靶向survivin的反义寡核苷酸对肿瘤细胞增殖的抑制作用 [Ｊ]. 中国生物化学与分子生物学报, 2001; 17(6)∶795.
8. 罗顺峰, 朱虹, 张万广, 等. 5FU对肝癌细胞内凋亡抑制蛋白survivin的诱导作用 [Ｊ]. 肝胆外科杂志, 2002; 10(3)∶227.
9. Ikeguchi M, Liu J, Kaibara N. Expression of survivin mRNA and protein in gastric cancer cell line (MKN45) during cisplatin treatment [Ｊ]. Apoptosis, 2002; 7(1)∶23.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Study on Gallbladder Carcinoma Apoptosis Induced by Antisense Oligodeoxynucleotide Targeting survivin

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