Expression of Aromatase and survivin and Their Clinicopathologic Significance in Primary Hepatocarcinoma_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

XIANG Zhigang ¹ , YANG Zhulin ² , DENG Xinghui. ²

Medical College of Jishou University, Jishou 416000, China;

Corresponding author：

Keywords：

Primary hepatocarcinoma Aromatase survivin Immunohistochemistry

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【Abstract】ObjectiveTo investigate the expressions of aromatase (Arom) and survivin (Surv) in primary hepatocarcinoma(PHC), and explore their relationships with the clinicopathology of PHC.
MethodsThe specimens from 47 patients with PHC were fixed in 10% formalin and routinely embedded in paraffin. The specimens were continuously sliced into 4 μmthick sections. ABC immunohistochemistry was performed to detect the expressions of Arom and Surv with polyclonal antibodies and scored them under highpower microscopy.
Results The positive rates and the scores of Arom and Surv in cancer tissues were significantly higher than those of the paratumor tissues 〔Arom: 40.43% vs 21.28% (P＜0.05), 1.53±1.69 vs 0.79±1.41 (P＜0.05); Surv: 63.83% vs 31.91% (P＜0.01), 2.40±1.96 vs 1.45±1.80 (P＜0.05)〕. The score of Surv in tumors with the maximal diameter ＜5 cm (4.00±2.10) was significantly higher than that in tumors with the maximal diameter ≥5 cm (2.17±1.86)， P＜0.05. However, there was no relationship between the expressions of Arom and Surv in PHCs and other clinicopathologic features of the PHCs. The positive correlation was found between the score of Arom and that of Surv in PHCs （r=0.316，P＜0.05）.
ConclusionThe expressions of Arom and Surv might be closely related to the carcinogenesis and development of PHC.

Citation： XIANG Zhigang,YANG Zhulin,DENG Xinghui.. Expression of Aromatase and survivin and Their Clinicopathologic Significance in Primary Hepatocarcinoma. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2006, 13(3): 321-324. doi: Copy

1.	Harada N, Ota H, Yoshimura N, et al. Localized aberrant expression of cytochrome P450 aromatase in primary and metastatic malignant tumors of human liver [Ｊ]. J Clin Endocrinol Metab, 1998; 83(2)∶ 697.
2.	Kappler M, Kotzsch M, Bartel F, et al. Elevated expression level of survivin protein in softtissue sarcomas is a strong independent predictor of survival [Ｊ]. Clin Cancer Res, 2003; 9(3)∶1098.
3.	胡淳玲，喻伦银，陈德基，等. 大鼠试验性肺鳞癌癌变各阶段间质微血管密度及VEGF、FLK1表达的动态变化 [Ｊ]. 癌症， 2001; 20(10)∶713.
4.	Meinhardt U, Mullis PE. The aromatase cytochrome P450 and its clinical impact [Ｊ]. Horm Res, 2002; 57(5-6)∶145.
5.	Castagnetta LA, Agostara B, Montalto G, et al. Local estrogen formation by nontumoral, cirrhotic, and malignant human liver tissues and cells[Ｊ]. Cancer Res, 2003; 63(16)∶5041.
6.	Shin S, Sung BJ, Cho YS, et al. An antiapoptotic protein human survivin is a direct inhibitor of caspase3 and 7[Ｊ]. Biochemistry, 2001; 40(4)∶1117.
7.	林木生，伊新，缪辉来，等. 生存素和天冬氨酸特异性半胱氨酸蛋白酶3在肝癌中的表达及其关系[Ｊ]. 中华实验外科杂志, 2004; 21(3)∶210.
8.	Shekhar MP, Werdell J, Tait L. Interaction with endothelial cells is a prerequisite for branching ductalalveolar morphogenesis and hyperplasia of preneoplastic human breast epithelial cells: regulation by estrogen[Ｊ]. Cancer Res, 2000;60(2)∶439.
9.	Kirma N, Gill K, Mandava U, et al. Overexpression of aromatase leads to hyperplasia and changes in the expression of genes involved in apoptosis, cell cycle, growth, and tumor suppressor functions in the mammary glands of transgenic mice [Ｊ]. Cancer Res, 2001; 61(5)∶1910.
10.	Thiantanawat A, Long BJ, Brodie AM. Signaling pathways of apoptosis activated by aromatase inhibitors and antiestrogens[Ｊ]. Cancer Res, 2003; 63(22)∶8037.

1. Harada N, Ota H, Yoshimura N, et al. Localized aberrant expression of cytochrome P450 aromatase in primary and metastatic malignant tumors of human liver [Ｊ]. J Clin Endocrinol Metab, 1998; 83(2)∶ 697.
2. Kappler M, Kotzsch M, Bartel F, et al. Elevated expression level of survivin protein in softtissue sarcomas is a strong independent predictor of survival [Ｊ]. Clin Cancer Res, 2003; 9(3)∶1098.
3. 胡淳玲，喻伦银，陈德基，等. 大鼠试验性肺鳞癌癌变各阶段间质微血管密度及VEGF、FLK1表达的动态变化 [Ｊ]. 癌症， 2001; 20(10)∶713.
4. Meinhardt U, Mullis PE. The aromatase cytochrome P450 and its clinical impact [Ｊ]. Horm Res, 2002; 57(5-6)∶145.
5. Castagnetta LA, Agostara B, Montalto G, et al. Local estrogen formation by nontumoral, cirrhotic, and malignant human liver tissues and cells[Ｊ]. Cancer Res, 2003; 63(16)∶5041.
6. Shin S, Sung BJ, Cho YS, et al. An antiapoptotic protein human survivin is a direct inhibitor of caspase3 and 7[Ｊ]. Biochemistry, 2001; 40(4)∶1117.
7. 林木生，伊新，缪辉来，等. 生存素和天冬氨酸特异性半胱氨酸蛋白酶3在肝癌中的表达及其关系[Ｊ]. 中华实验外科杂志, 2004; 21(3)∶210.
8. Shekhar MP, Werdell J, Tait L. Interaction with endothelial cells is a prerequisite for branching ductalalveolar morphogenesis and hyperplasia of preneoplastic human breast epithelial cells: regulation by estrogen[Ｊ]. Cancer Res, 2000;60(2)∶439.
9. Kirma N, Gill K, Mandava U, et al. Overexpression of aromatase leads to hyperplasia and changes in the expression of genes involved in apoptosis, cell cycle, growth, and tumor suppressor functions in the mammary glands of transgenic mice [Ｊ]. Cancer Res, 2001; 61(5)∶1910.
10. Thiantanawat A, Long BJ, Brodie AM. Signaling pathways of apoptosis activated by aromatase inhibitors and antiestrogens[Ｊ]. Cancer Res, 2003; 63(22)∶8037.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Expression of Aromatase and survivin and Their Clinicopathologic Significance in Primary Hepatocarcinoma

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