Inhibition Effects of Nimesulide on Proliferation in Human Cholangiocarcinoma Cell Line QBC939 in Vitro_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

HUANG Qiang ¹ , KONG Qi ² , ZHAI Zhimin. ³

Department of General Surgery, Affiliated Provincial Hospital of Anhui Medical University, Hefei 230032, China;

Corresponding author：

Keywords：

Cyclooxygenase-2 Nimesulide Cholangiocarcinoma Proliferation Apoptosis

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

【Abstract】ObjectiveTo investigate the effects of nimesulide, a selective cyclooxygenase-2 (COX-2) inhibitor, on human cholangiocarcinoma QBC939 cell line in vitro.
MethodsThe effects of nimesulide on QBC939 cells were observed with the following techniques: the influence of nimesulide on the proliferation of QBC939 cells was determined by MTT assay; the apoptosis of QBC939 cells was viewed and measured by transmission electron microscopy and flow cytometry, respectively; the expressions of proliferation cell nuclear antigen (PCNA) and COX-2 of cholangiocarcinoma cells were detected by immunocytochemistry.
ResultsNimesulide inhibited the expressions of PCNA and COX-2 and the proliferation of cholangiocarcinoma QBC939 cells, whose effects intensified as the dose increased and time elongated. Flow cytometry showed that the apoptotic rates of QBC939 cells increased significantly as the dose of nimesulide increased. The typical morphologic features of apoptosis were also observed by transmission electron microscopy.
ConclusionNimesulide significantly inhibits the proliferation of QBC939 cells in vitro by inducting cell apoptosis, which may be associated with the downregulation of COX-2 expression, and it also presents the features of dose and time dependents.

Citation： HUANG Qiang,KONG Qi,ZHAI Zhimin.. Inhibition Effects of Nimesulide on Proliferation in Human Cholangiocarcinoma Cell Line QBC939 in Vitro. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2006, 13(4): 413-416. doi: Copy

1.	Hepatology, 2001; 33(6)∶1353.
2.	Hepatology, 2003; 38(3)∶756.
3.	Hepatobiliary Pancreat Dis Int, 2002; 1(3)∶429.
4.	Characterization of Prostaglandin G/H Synthase 1 and 2 in rat, dog, monkey, and human gastrointestinal tracts [J].
5.	Gastroenterology, 1996; 111(2)∶445.
6.	Proapoptotic and antiproliferative potential of selective cyclooxygenase2 inhibitors in human liver tumor cells [J].
7.	Hepatology, 2002; 36(4 Pt 1)∶885.
8.	Sulindac and exisulind exhibit a significant antiproliferative effect and induce apoptosis in human hepatocellular carcinoma cell lines [J].
9.	Cancer Res, 2000; 60(8)∶2085.
10.	Patel T. Increasing incidence and mortality of primary intrahepatic cholangiocarcinoma in the United States [J].
11.	李玉民，薛群基，周彦明，等. 胃癌组织中环氧合酶2的表达及其意义 [J]. 中国普外基础与临床杂志， 2003； 10(2)∶137.
12.	Li JY, Wang XZ, Chen FL, et al. Nimesulide inhibits proliferation via induction of apoptosis and cell cycle arrest in human gastric adenocarcinoma cell line [J]. World J Gastroenterol, 2003; 9(5)∶915.
13.	Leng J, Han C, Demetris AJ, et al. Cyclooxygenase2 promotes hepatocellular carcinoma cell growth through Akt activation: evidence for Akt inhibition in celecoxibinduced apoptosis [J].
14.	Wu GS, Wang JH, Liu ZR, et al. Expression of cyclooxygenase1 and2 in extrahepatic cholangiocarcinoma [J].
15.	Kargman S, Charleson S, Cartwright M, et al.
16.	Kern MA, Schubert D, Sahi D, et al.
17.	Rahman MA, Dhar DK, Masunaga R, et al.
18.	吴高松，刘正人，邹声泉, 等. 塞来昔布(celecoxib)通过前列腺素E2途径影响胆管癌细胞株生长和凋亡 [J]. 外科理论与实践， 2003； 8（2）∶137.

1. Hepatology, 2001; 33(6)∶1353.
2. Hepatology, 2003; 38(3)∶756.
3. Hepatobiliary Pancreat Dis Int, 2002; 1(3)∶429.
4. Characterization of Prostaglandin G/H Synthase 1 and 2 in rat, dog, monkey, and human gastrointestinal tracts [J].
5. Gastroenterology, 1996; 111(2)∶445.
6. Proapoptotic and antiproliferative potential of selective cyclooxygenase2 inhibitors in human liver tumor cells [J].
7. Hepatology, 2002; 36(4 Pt 1)∶885.
8. Sulindac and exisulind exhibit a significant antiproliferative effect and induce apoptosis in human hepatocellular carcinoma cell lines [J].
9. Cancer Res, 2000; 60(8)∶2085.
10. Patel T. Increasing incidence and mortality of primary intrahepatic cholangiocarcinoma in the United States [J].
11. 李玉民，薛群基，周彦明，等. 胃癌组织中环氧合酶2的表达及其意义 [J]. 中国普外基础与临床杂志， 2003； 10(2)∶137.
12. Li JY, Wang XZ, Chen FL, et al. Nimesulide inhibits proliferation via induction of apoptosis and cell cycle arrest in human gastric adenocarcinoma cell line [J]. World J Gastroenterol, 2003; 9(5)∶915.
13. Leng J, Han C, Demetris AJ, et al. Cyclooxygenase2 promotes hepatocellular carcinoma cell growth through Akt activation: evidence for Akt inhibition in celecoxibinduced apoptosis [J].
14. Wu GS, Wang JH, Liu ZR, et al. Expression of cyclooxygenase1 and2 in extrahepatic cholangiocarcinoma [J].
15. Kargman S, Charleson S, Cartwright M, et al.
16. Kern MA, Schubert D, Sahi D, et al.
17. Rahman MA, Dhar DK, Masunaga R, et al.
18. 吴高松，刘正人，邹声泉, 等. 塞来昔布(celecoxib)通过前列腺素E2途径影响胆管癌细胞株生长和凋亡 [J]. 外科理论与实践， 2003； 8（2）∶137.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Inhibition Effects of Nimesulide on Proliferation in Human Cholangiocarcinoma Cell Line QBC939 in Vitro

Abstract Full text Figures/Tables Video References Cited by

Format

Content