【Abstract】ObjectiveTo discuss the possibility and clinical significance of SSX-1 mRNA used as specific marker for examining HCC cells in peripheral blood of HCC patients.
MethodsUsing the RT-PCR method, the SSX1 mRNA of the peripheral blood was examined in 25 cases of HCC patients and 20 non-HCC patients. The same method was used to detect the expression of SSX-1 mRNA in the tumor tissues, para-tumor tissues, cirrhosis tissues and normal hepatic tissues. A randomized sample was extracted for DNA sequencing from positive electrophoresis expression samples of SSX-1 to examine the reliability of results.
ResultsThe expression rates of SSX-1 mRNA were 60%(15/25) and 40%（10/25） respectively in tumor tissues of HCC and the corresponding peripheral blood. SSX-1 mRNA were not expressed in para-tumor tissues,cirrhosis tissues and normal hepatic tissues. The DNA sequence -confirmed that the RTPCR products were true target cDNA. No relationships were found between the expression of SSX-1 gene and clinical characteristics, such as age, sex, tumor size, TNM stage， extent of differentiation and serum AFP level (P gt;0.05). However, in 33%（3/9） patients with normal serum AFP ( lt;20 μg/L), specific expression of SSX1 mRNA was observed.
ConclusionHigh specific expression of SSX-1 mRNA is observed in the peripheral blood of patients with HCC, it suggests that applying it as a tumor marker to detect HCC cells in peripheral blood is an adjuvant diagnostic tool. The expression of SSX-1 mRNA in the peripheral blood is observed in the group HCC patients whose serum AFP ( lt;20 μg/L) are normal, which suggests that applying both SSX-1 mRNA and AFP as tumor markers together might be useful to improve the diagnostic accuracy for HCC.
Citation：
WANG Xinjian,XIA Min,WANG Lianbo.. Detection of Hepatocellular Carcinoma Cells Using SSX1 mRNA as Tumor Specific Marker. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2006, 13(4): 437-440. doi:
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Copyright © the editorial department of CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY of West China Medical Publisher. All rights reserved
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- 1. Dos Santos NR, Torensma R, de Vries TJ, et al. Heterogeneous expression of the SSX cancer/testis antigens in human melanoma lesions and cell lines [Ｊ]. Cancer Res, 2000; 60(6)∶ 1654.
- 2. Chen CH, Chen GJ, Lee HS, et al. Expressions of cancertestis antigens in human hepatocellular carcinomas [Ｊ]. Cancer Lett, 2001; 164（2）∶189.
- 3. 徐从高， 张茂宏， 杨兴季， 等主译. 癌肿瘤学原理和实践 [M]. 第5版. 济南: 山东科学技术出版社, 2001∶1123～1150.
- 4. Ghossein RA, Osman I, Bhattacharya S, et al. Detection of prostatic specific membrane antigen messenger RNA using immunobead reverse transcriptase polymerase chain reaction [Ｊ]. Diagn Mol Pathol, 1999; 8(2)∶59.
- 5. von Knebel DoeberitzM, Lacroix J. Nucleic acid based techniques for the detection of rare cancer cells in clinical samples [Ｊ]. Cancer Metastasis Rev, 1999; 18(1)∶43.
- 6. Mori M, Mimori K, Ueo H, et al. Clinical significance of molecular detection of carcinoma cells in lymph nodes and peripheral blood by reverse transcriptionpolymerase chain reaction in patients with gastrointestinal or breast carcinomas [Ｊ]. J Clin Oncol, 1998； 16（1）∶128.
- 7. Yamaguchi K, Takagi Y, Aoki S, et al. Significant detection of circulating cancer cells in the blood by reverse transcriptasepolymerase chain reaction during colorectal cancer resection [Ｊ]. Ann Surg, 2000； 232(1)∶58.
- 8. Müller C, Petermann D, Pfeffel F, et al. Lack of specificity of albuminmRNApositive cells as a marker of circulating hepatoma cells [Ｊ]. Hepatology, 1997; 25(4)∶896.
- 9. Lemoine A, LeBricon T, Salvucci M, et al. Prospective evaluation of circulating hepatocytes by alphafetoprotein mRNA in humans during liver surgery [Ｊ]. Ann Surg, 1997; 226(1)∶43.
- 10. Luo G, Hang S, Xie X, et al. Expression of cancertestis genes in human hepatocellular carcinomas[Ｊ]. Cancer Immun, 2002; 2∶11.
- 11. 牟东成， 冷希圣， 彭吉润， 等.肿瘤睾丸抗原SSX1基因mRNA在肝细胞肝癌中的表达 [Ｊ]. 中华实验外科杂志， 2003; 19(2)∶110.
