Detection of Hepatocellular Carcinoma Cells Using SSX1 mRNA as Tumor Specific Marker_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

WANG Xinjian , XIA Min , WANG Lianbo.

Department of Hepatobiliary Surgery, Central Hospital of Wendeng City, Shandong Province， Wendeng 264400, China;

Corresponding author：

Keywords：

Hepatocellular carcinoma Reverse-transcriptase polymerase chain reaction SSX-1 gene

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【Abstract】ObjectiveTo discuss the possibility and clinical significance of SSX-1 mRNA used as specific marker for examining HCC cells in peripheral blood of HCC patients.
MethodsUsing the RT-PCR method, the SSX1 mRNA of the peripheral blood was examined in 25 cases of HCC patients and 20 non-HCC patients. The same method was used to detect the expression of SSX-1 mRNA in the tumor tissues, para-tumor tissues, cirrhosis tissues and normal hepatic tissues. A randomized sample was extracted for DNA sequencing from positive electrophoresis expression samples of SSX-1 to examine the reliability of results.
ResultsThe expression rates of SSX-1 mRNA were 60%(15/25) and 40%（10/25） respectively in tumor tissues of HCC and the corresponding peripheral blood. SSX-1 mRNA were not expressed in para-tumor tissues,cirrhosis tissues and normal hepatic tissues. The DNA sequence -confirmed that the RTPCR products were true target cDNA. No relationships were found between the expression of SSX-1 gene and clinical characteristics, such as age, sex, tumor size, TNM stage， extent of differentiation and serum AFP level (P gt;0.05). However, in 33%（3/9） patients with normal serum AFP ( lt;20 μg/L), specific expression of SSX1 mRNA was observed.
ConclusionHigh specific expression of SSX-1 mRNA is observed in the peripheral blood of patients with HCC, it suggests that applying it as a tumor marker to detect HCC cells in peripheral blood is an adjuvant diagnostic tool. The expression of SSX-1 mRNA in the peripheral blood is observed in the group HCC patients whose serum AFP ( lt;20 μg/L) are normal, which suggests that applying both SSX-1 mRNA and AFP as tumor markers together might be useful to improve the diagnostic accuracy for HCC.

Citation： WANG Xinjian,XIA Min,WANG Lianbo.. Detection of Hepatocellular Carcinoma Cells Using SSX1 mRNA as Tumor Specific Marker. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2006, 13(4): 437-440. doi: Copy

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1. Dos Santos NR, Torensma R, de Vries TJ, et al. Heterogeneous expression of the SSX cancer/testis antigens in human melanoma lesions and cell lines [Ｊ]. Cancer Res, 2000; 60(6)∶ 1654.
2. Chen CH, Chen GJ, Lee HS, et al. Expressions of cancertestis antigens in human hepatocellular carcinomas [Ｊ]. Cancer Lett, 2001; 164（2）∶189.
3. 徐从高，张茂宏，杨兴季，等主译. 癌肿瘤学原理和实践 [M]. 第5版. 济南: 山东科学技术出版社, 2001∶1123～1150.
4. Ghossein RA, Osman I, Bhattacharya S, et al. Detection of prostatic specific membrane antigen messenger RNA using immunobead reverse transcriptase polymerase chain reaction [Ｊ]. Diagn Mol Pathol, 1999; 8(2)∶59.
5. von Knebel DoeberitzM, Lacroix J. Nucleic acid based techniques for the detection of rare cancer cells in clinical samples [Ｊ]. Cancer Metastasis Rev, 1999; 18(1)∶43.
6. Mori M, Mimori K, Ueo H, et al. Clinical significance of molecular detection of carcinoma cells in lymph nodes and peripheral blood by reverse transcriptionpolymerase chain reaction in patients with gastrointestinal or breast carcinomas [Ｊ]. J Clin Oncol, 1998； 16（1）∶128.
7. Yamaguchi K, Takagi Y, Aoki S, et al. Significant detection of circulating cancer cells in the blood by reverse transcriptasepolymerase chain reaction during colorectal cancer resection [Ｊ]. Ann Surg, 2000； 232(1)∶58.
8. Müller C, Petermann D, Pfeffel F, et al. Lack of specificity of albuminmRNApositive cells as a marker of circulating hepatoma cells [Ｊ]. Hepatology, 1997; 25(4)∶896.
9. Lemoine A, LeBricon T, Salvucci M, et al. Prospective evaluation of circulating hepatocytes by alphafetoprotein mRNA in humans during liver surgery [Ｊ]. Ann Surg, 1997; 226(1)∶43.
10. Luo G, Hang S, Xie X, et al. Expression of cancertestis genes in human hepatocellular carcinomas[Ｊ]. Cancer Immun, 2002; 2∶11.
11. 牟东成，冷希圣，彭吉润，等.肿瘤睾丸抗原SSX1基因mRNA在肝细胞肝癌中的表达 [Ｊ]. 中华实验外科杂志， 2003; 19(2)∶110.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Detection of Hepatocellular Carcinoma Cells Using SSX1 mRNA as Tumor Specific Marker

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