Experimental Study on Liver Regeneration Following Portal Branch Ligation in Rats_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

GUO Meixiang ¹

Department of General Surgery, People’s Hospital of Laizhou City, Laizhou 261400, China;

Corresponding author：

Keywords：

LigationPortal branchLiver regeneration

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【Abstract】　Objective　To study liver regeneration of the non-ligated liver lobes following portal branch ligation (PBL). Methods　 Sixty male Wistar rats were randomly divided into PBL group and sham operation (SO) group. Under ether anesthesia, the rats were subjected to PBL and sham operation, respectively. The animals were sacrificed on the 1st, 2nd, 3rd, 7th and 14th day respectively. The blood sample was collected from heart and the livers were harvested to determine serum alanine aminotransferase (ALT) levels and total liver weight, respectively. The hepatic histopathology was studied through light microscopy. The number of liver cell nuclear mitosis index was counted. The number of proliferative cell nuclear antigen (PCNA) index was counted by immunohistochemistry. The hepatic ultrastructural changes were studied under electron microscope. Results　①Elevated serum ALT level was observed in the first postoperative day in PBL group compared with SO group (P＜0.01)， but began to recover in the second day. ②No significant total liver weight change in PBL group and SO group were found. ③Liver cell nuclear mitosis index and PCNA index were markedly increased in PBL group compared with SO group in day 1-3 postoperative day (P＜0.01). It reached the peak in the second day and decreased slightly in the 3rd day, but still higher than SO group, then gradually return to normal lately. Conclusion　The ligation of left portal branch can induce active regeneration of hepatic cell of non-ligated liver lobes in rats. The regeneration of non-ligated liver lobes may restore previous total liver weight. The ligation of 75% portal branch does not affect liver function and may be safely performed. The portal branch ligation in rats may be used as an animal model in study of liver regeneration.

Citation： GUO Meixiang,GAO Lihua,LIU Libo,MENG Zhaohua,GONG Xiumei.. Experimental Study on Liver Regeneration Following Portal Branch Ligation in Rats. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2006, 13(5): 550-554. doi: Copy

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13.	Elias D, Debaere T, Roche A, et al. Preoperative selective portal vein embolizations are an effective means of extending the indications of major hepatectomy in the normal and injured liver \[J\]. Hepatogastroenterology， 1998; 45(19)∶170.
14.	Taniguchi H, Daidoh T, Shioaki Y, et al. Blood supply and drug delivery to primary and secondary human liver cancers studied with in vivo bromodeoxyuridine labeling\[J\]. Cancer, 1993； 71（1）∶50.
15.	Kinoshita H, Sakai K, Hirohashi K, et al. Preoperative portal vein embolization for hepatocellular carcinoma \[J\].World J Surg， 1986; 10(5)∶803.
16.	Castaing D, Garden OJ, Bismuth H. Segmental liver resection using ultrasound-guided selective portal venous occlusion \[J\]. Ann Surg， 1989; 210(1)∶20.
17.	Matsumata T, Kanematsu T, Takenaka K, et al. Lack of intrahepatic recurrence of hepatocellular carcinoma by temporary portal venous embolization with starch microspheres \[J\]. Surgery， 1989; 105(2 Pt 1)∶188.

1. 　Tanaka H, Hirohashi K, Kubo S, et al. Preoperative portal vein embolization improves prognosis after right hepatectomy for hepatocellular carcinoma in patients with impaired hepatic function \[J\]. Br J Surg， 2000; 87(7)∶879.
2. 　葛敦钧，钱　峰. 肝癌预治疗后二期切除时机的探讨 \[J\]. 中华肝胆外科杂志， 1998; 5（5）∶331.
3. 　Shimamura T, Nakajima Y, Une Y, et al. Efficacy and safety of preoperative percutaneous transhepatic portal embolization with absolute ethanol: a clinical study \[J\]. Surgery， 1997; 121(2)∶135.
4. 　汤钊猷主编. 原发性肝癌研究与进展 \[M\]. 上海: 上海医科大学出版社， 1990∶86~89.
5. 　陆　森，钱建民，黄海清，等. 几种不同预测大鼠肝脏体积（重量）方法的比较 \[J\]. 中华实验外科杂志， 2002; 19（3）∶252.
6. 　Rocheleau B, Ethier C, Houle R, et al. Hepatic artery buffer response following left portal vein ligation: its role in liver tissue homeostasis \[J\]. Am J Physiol， 1999; 277(5 Pt 1)∶G1000.
7. 　Yokoyama Y, Baveja R, Sonin N, et al. Hepatic neovascularization after partial portal vein ligation: novel mechanism of chronic regulation of blood flow \[J\]. Am J Physiol Gastrointest Liver Physiol， 2001; 280(1)∶21.
8. 　Bilodeau M, Aubry MC, Houle R, et al. Evaluation of hepatocyte injury following partial ligation of the left portal vein \[J\]. J Hepatol， 1999; 30(1)∶29.
9. 　Fausto N, Laird AD, Webber EM. Liver regeneration. 2. Role of growth factors and cytokines in hepatic regeneration \[J\]. FASEB J， 1995; 9(15)∶1527.
10. 　Picard C, Starkel P, Sempoux C, et al. Molecular mechanisms of apoptosis in the liver of rats after portal branch ligation with and without retrorsine \[J\]. Lab Invest， 2004; 84(5)∶618.
11. 　Hashimoto M, Watanabe G. Changes in functional capacity after portal branch ligation in rats \[J\]. Surg Today， 2001; 31(12)∶1065.
12. 　Schwartz JD, Schwartz M, Mandeli J, et al. Neoadjuvant and adjuvant therapy for resectable hepatocellular carcinoma: review of the randomised clinical trials \[J\]. Lancet Oncol， 2002; 3(10)∶593.
13. 　Elias D, Debaere T, Roche A, et al. Preoperative selective portal vein embolizations are an effective means of extending the indications of major hepatectomy in the normal and injured liver \[J\]. Hepatogastroenterology， 1998; 45(19)∶170.
14. 　Taniguchi H, Daidoh T, Shioaki Y, et al. Blood supply and drug delivery to primary and secondary human liver cancers studied with in vivo bromodeoxyuridine labeling\[J\]. Cancer, 1993； 71（1）∶50.
15. 　 Kinoshita H, Sakai K, Hirohashi K, et al. Preoperative portal vein embolization for hepatocellular carcinoma \[J\].World J Surg， 1986; 10(5)∶803.
16. 　Castaing D, Garden OJ, Bismuth H. Segmental liver resection using ultrasound-guided selective portal venous occlusion \[J\]. Ann Surg， 1989; 210(1)∶20.
17. 　Matsumata T, Kanematsu T, Takenaka K, et al. Lack of intrahepatic recurrence of hepatocellular carcinoma by temporary portal venous embolization with starch microspheres \[J\]. Surgery， 1989; 105(2 Pt 1)∶188.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Experimental Study on Liver Regeneration Following Portal Branch Ligation in Rats

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