Alleviating Ischemia-Reperfusion Injury in Aged Liver by Inducing of Heme Oxygenase-1: Ameliorating Oxidative Stress_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

WANG Ke , WANG Xuehao , ZHANG Feng , LI Xiangcheng , QIAN Xiaofeng

The Liver Transplantation Center of Jiangsu Province, Department of Hepatic Surgery, The First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China;

Corresponding author：

Keywords：

Aged donor Heme oxygenase-1 Ischemia-reperfusion injury

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

【Abstract】ObjectiveTo investigate whether heme oxygenase-1 can alleviate the ischemiareperfusion injury of the aged donor liver.
MethodsThe activity of superoxide dismutase (SOD) and catalase (CAT), and the contents of tocopherol (Vit E), ascorbic acid (Vit C) and malondialdehyde (MDA) were measured in the livers of adult SD rats (n=5) and aged SD rats (n=5). The experimental aged donor group (n=30) received intraperitoneal injection of Hemin 24 hours before operation, the control aged donor group(n=30) received saline. The histologic changes and apoptosis in the donor liver were observed.
ResultsThe activity of SOD and the contents of Vit E and Vit C decreased significantly in 5 aged rats（P＜0.05）, but the content of MDA increased（P＜0.05）. Before the harvesting of the grafts, the activity of SOD and the contents of Vit E and Vit C increased significantly in rats pretreated with Hemin （P＜0.05） and the content of MDA decreased（P＜0.05）. The apoptotic cells in the livers pretreated with Hemin also decreased significantly after reperfusion（P＜0.05）.
ConclusionThe liver of aged rat presents oxidative stress and peroxidative state. Ischemia-reperfusion injury can be alleviated by the induction of HO-1.

Citation： WANG Ke,WANG Xuehao,ZHANG Feng,LI Xiangcheng,QIAN Xiaofeng,FAN Ye.. Alleviating Ischemia-Reperfusion Injury in Aged Liver by Inducing of Heme Oxygenase-1: Ameliorating Oxidative Stress. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2005, 12(2): 109-112. doi: Copy

1.	Kohli V, Selzner M, Madden JF, et al. Endothelial cell and hepatocyte deaths occur by apoptosis after ischemiareperfusion injury in the rat liver [Ｊ]. Transplantation, 1999; 67(8)1099.
2.	Deschenes M, Forbes C, Tchervenkov J, et al. Use of older donor livers is associated with more extensive ischemic damage on intraoperative biopsies during liver transplantation [Ｊ]. Liver Transpl Surg, 1999; 5(5)357.
3.	Busuttil RW, Tanaka K. The utility of marginal donors in liver transplantation [Ｊ]. Liver Transpl, 2003; 9(7)651.
4.	Yersiz H, Shaked A, Olthoff K, et al. Correlation between donor age and the pattern of liver graft recovery after transplantation [Ｊ]. Transplantation, 1995; 60(8)790.
5.	Busquets J, Xiol X, Figueras J, et al. The impact of donor age on liver transplantation: influence of donor age on early liver function and on subsequent patient and graft survival [Ｊ]. Transplantation, 2001; 71(12)1765.
6.	Briceno J, Marchal T, Padillo J, et al. Influence of marginal donors on liver preservation injury [Ｊ]. Transplantation, 2002; 74(4)522.
7.	Sass G, Soares MC, Yamashita K, et al. Heme oxygenase1 and its reaction product, carbon monoxide, prevent inflammationrelated apoptotic liver damage in mice [Ｊ]. Hepatology, 2003; 38(4)909.
8.	Kato Y, Shimazu M, Kondo M, et al. Bilirubin rinse: A simple protectant against the rat liver graft injury mimicking heme oxygenase1 preconditioning [Ｊ]. Hepatology, 2003; 38(2)364.
9.	Schwartzman RA, Cidlowski JA. Apoptosis: the biochemistry and molecular biology of programmed cell death [Ｊ]. Endocr Rev, 1993; 14(2)133.

1. Kohli V, Selzner M, Madden JF, et al. Endothelial cell and hepatocyte deaths occur by apoptosis after ischemiareperfusion injury in the rat liver [Ｊ]. Transplantation, 1999; 67(8)1099.
2. Deschenes M, Forbes C, Tchervenkov J, et al. Use of older donor livers is associated with more extensive ischemic damage on intraoperative biopsies during liver transplantation [Ｊ]. Liver Transpl Surg, 1999; 5(5)357.
3. Busuttil RW, Tanaka K. The utility of marginal donors in liver transplantation [Ｊ]. Liver Transpl, 2003; 9(7)651.
4. Yersiz H, Shaked A, Olthoff K, et al. Correlation between donor age and the pattern of liver graft recovery after transplantation [Ｊ]. Transplantation, 1995; 60(8)790.
5. Busquets J, Xiol X, Figueras J, et al. The impact of donor age on liver transplantation: influence of donor age on early liver function and on subsequent patient and graft survival [Ｊ]. Transplantation, 2001; 71(12)1765.
6. Briceno J, Marchal T, Padillo J, et al. Influence of marginal donors on liver preservation injury [Ｊ]. Transplantation, 2002; 74(4)522.
7. Sass G, Soares MC, Yamashita K, et al. Heme oxygenase1 and its reaction product, carbon monoxide, prevent inflammationrelated apoptotic liver damage in mice [Ｊ]. Hepatology, 2003; 38(4)909.
8. Kato Y, Shimazu M, Kondo M, et al. Bilirubin rinse: A simple protectant against the rat liver graft injury mimicking heme oxygenase1 preconditioning [Ｊ]. Hepatology, 2003; 38(2)364.
9. Schwartzman RA, Cidlowski JA. Apoptosis: the biochemistry and molecular biology of programmed cell death [Ｊ]. Endocr Rev, 1993; 14(2)133.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Alleviating Ischemia-Reperfusion Injury in Aged Liver by Inducing of Heme Oxygenase-1: Ameliorating Oxidative Stress

Abstract Full text Figures/Tables Video References Cited by

Format

Content