Impact of Panax Notoginseng Saponins Preconditioning on Apoptosis and Expression of Bcl-2 and Caspase-3 in Rat Liver Transplantation_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

ZHANG Yi ¹ , YE Qifa ² , ZHOU Jinxue ¹ , MING Yingzi ² , XU Xianlin ¹ , XIAO Jiansheng. ¹

Institute of Organ Transplantation, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China;

Corresponding author：

Keywords：

Panax notoginseng saponinsRatLiver transplantationBcl-2Caspase-3

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【Abstract】ObjectiveTo investigate the protective effects and the impact on the expression of Bcl-2 and Caspase-3 mRNA by Panax notoginseng saponins (PNS) preconditioning in rat liver transplantation.
MethodsMale Sprague-Dawley rats were used as donors and recipients of orthotopic liver transplantation (OLT) and were divided into PNS preconditioning group (PNS group) and NS control group (NS group) randomly according to whether PNS was injected by venous (50 mg/kg) 1 h before liver grafts harvesting. The animals were respectively killed 2 h, 6 h and 24 h after reperfusion. Plasma samples were collected for ALT and AST test. Liver tissues were collected to detect histological changes, apoptosis and the expression of Bcl-2, Caspase-3 mRNA.
ResultsThe serum levels of ALT and AST and the apoptosis index (AI) of liver tissue in PNS group were lower than those in NS group’s significantly (P＜0.05) at 2 h, 6 h and 24 h after reperfusion. The expression of Bcl-2 mRNA was enhanced significantly in PNS group at 6 h, 24 h after reperfusion and the expression of Caspase-3 mRNA was decreased significantly in PNS group at 2 h, 6 h after reperfusion as compared with NS group’s(P＜0.05).
ConclusionPNS preconditioning can attenuate liver grafts ischemia/reperfusion injury and apoptosis of hepatocytes. Affecting expression of Bcl-2 and Caspase-3 genes may be one of the mechanisms of PNS antiapoptotic effects.

Citation： ZHANG Yi,YE Qifa,ZHOU Jinxue,MING Yingzi,XU Xianlin,XIAO Jiansheng.. Impact of Panax Notoginseng Saponins Preconditioning on Apoptosis and Expression of Bcl-2 and Caspase-3 in Rat Liver Transplantation. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2005, 12(2): 113-116下转126. doi: Copy

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1. Rudiger HA, Graf R, Clavien PA. Liver ischemia: apoptosis as a central mechanism of injury [Ｊ]. J Invest Surg, 2003; 16(3)149.
2. Kamada N, Calne RY. Orthotopic liver transplantation in the rat. Technique using cuff for portal vein anastomosis and biliary drainage [Ｊ]. Transplantation, 1979; 28(1)47.
3. Kohli V, Selzner M, Madden JF, et al. Endothelial cell and hepatocyte deaths occur by apoptosis after ischemiareperfusion injury in the rat liver [Ｊ]. Transplantation, 1999; 67(8)1099.
4. 朱陵群，范吉平，黄启福，等. 三七总皂苷抗缺氧缺糖再给氧诱导大鼠海马神经细胞凋亡的研究 [Ｊ]. 中国中药杂志, 2003； 28（1）52.
5. Gross A, McDonnell JM, Korsmeyer SJ. BCL2 family members and the mitochondria in apoptosis [Ｊ]. Genes Dev, 1999; 13(15)1899.
6. Bilbao G, Contreras JL, Eckhoff DE, et al. Reduction of ischemiareperfusion injury of the liver by in vivo adenovirusmediated gene transfer of the antiapoptotic Bcl2 gene [Ｊ]. Ann Surg, 1999; 230(2)185.
7. SS〗（下转第126页）（上承第116页）.
8. Oshiro T, Shiraishi M, Muto Y. Adenovirus mediated gene transfer of antiapoptotic protein in hepatic ischemiareperfusion injury: the paradoxical effect of Bcl2 expression in the reperfused liver [Ｊ]. J Surg Res, 2002; 103(1)30.
9. Fumarola C, Guidotti GG. Stressinduced apoptosis: toward a symmetry with receptormediated cell death [Ｊ]. Apoptosis, 2004; 9(1)77.
10. Cursio R, Gugenheim J, Ricci JE, et al. Caspase inhibition protects from liver injury following ischemia and reperfusion in rats [Ｊ]. Transpl Int, 2000; 13(Suppl 1)S568.
11. 李巾伟，朱培纯，司银楚,等. 三七总皂甙对脑出血大鼠前脑促凋亡基因Caspase3影响的研究 [Ｊ]. 北京中医药大学学报, 2003； 26（2）22.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Impact of Panax Notoginseng Saponins Preconditioning on Apoptosis and Expression of Bcl-2 and Caspase-3 in Rat Liver Transplantation

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