Liposome Mediated Allograft Transduction of IL-10 in Rat Liver Transplantation_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

ZHANG Dong , GAO Dongchen , ZHANG Zhongtao , WANG Yu.

Department of General Surgery, Beijing Friendship Hospital Affiliated of Capital University of Medical Sciences, Beijing 100050, China;

Corresponding author：

Keywords：

Liver transplantation Interleukine-10 Liposome Gene therapy

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【Abstract】ObjectiveTo investigate the effect of genetic modulation of the hepatic graft with IL-10 during liver preservation in rat liver transplantation.
MethodsEleven cases of orthotopic liver transplantation were performed in Lewis to BN rats according to the cuff’s technique. All rats were divided into 3 groups,which were control group(n=3), Lipo group(n=4) and Lipo-rIL-10 group(n=4). Lipofectamine 2000-pCR3.1 complex and Lipofectamine 2000-pCR3.1 rIL10 complex were respectively injected into portal vein and kept for 45 minutes to transfect grafts during cold preservation in vitro. All rats were killed on postoperative day 6. Serum samples were collected for decting IL-10 by means ELISA. Transgene expression of rIL-10 was assessed by means of RT-PCR and immunohistochemistry.
ResultsIn Lipo-rIL-10 group, levels of IL-10 from suprahepatic vena cava were significant higher than those from infrahepatic vena cava (P=0.024), transgene expression of rIL-10 in Lipo-rIL-10 were higher than those of control group and Lipo group assessed by means of RT-PCR and immunohistochemistry.
ConclusionDuring cold preservation in vitro through portal vein injection to donor liver, liposome mediated gene transfection can successfully achieve local gene expression.

Citation： ZHANG Dong,GAO Dongchen,ZHANG Zhongtao,WANG Yu.. Liposome Mediated Allograft Transduction of IL-10 in Rat Liver Transplantation. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2005, 12(2): 123-126. doi: Copy

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1. Kamada N, Calne RY. Orthotopic liver transplantation in the rat： Technique using cuff for portal vein anastomosis and biliary drainage [Ｊ]. Transplantation， 1979; 28(1)∶47.
2. Kamada N, Sumimoto R, Kaneda K. The value of hepatic artery reconstruction as a technique in rat liver transplantation [Ｊ]. Surgery， 1992; 111(2)195.
3. Sen L, Hong YS, Luo H, et al. Efficiency, efficacy, and adverse effects of adenovirus vs. liposomemediated gene therapy in cardiac allografts [Ｊ]. Am J Physiol Heart Circ Physiol, 2001; 281(3)H1433.
4. Shinozaki K, Yahata H, Tanji H, et al. Allograft transduction of IL10 prolongs survival following orthotopic liver transplantation [Ｊ]. Gene Ther, 1999; 6(5)816.
5. Herweijer H, Wolff JA. Progress and prospects: naked DNA gene transfer and therapy [Ｊ]. Gene Ther, 2003; 10(6)453.
6. Kren BT, Chowdhury NR, Chowdhury JR, et al. Gene therapy as an alternative to liver transplantation [Ｊ]. Liver Transpl, 2002; 8(12)1089.
7. Itano H, Mora BN, Zhang W, et al. Lipidmediated ex vivo gene transfer of viral interleukin 10 in rat lung allotransplantation [Ｊ]. J Thorac Cardiovasc Surg, 2001; 122(1)29.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Liposome Mediated Allograft Transduction of IL-10 in Rat Liver Transplantation

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