【Abstract】ObjectiveTo investigate the effects and mechanism of Panax Notoginseng saponins (PNGS) on rat liver during cold preservation.
Methods Using isolated perfusion of rat liver model (IPRL), Fura-2 method was used to measur the concentration of calcium ion in hepatic cells which had been preserved in DMEM solution with different concentration of PNGS added and cold preserved for 2 hours. Liver function, metabolic products of oxygen free radicals, energy substance and aucount of biliary flow as well as morphological study were measured from liver tissue which had been cold preserved in lactate riuges’s solution containing different dosages of PNGS for 24 hours and 30 minutes reperfusion.
ResultsThe contents of intracellur calcium of the rat hepatocytes,MDA, AST, ALT, LDH were lower than those in the control group,but SOD, ATP, TAN, EC and bile production were higher than those in the control group(P＜0.01).During 200-600 mg,the protective effects of the PNGS were correlated with the dosage and manifest dosagedependence(P lt;0.01),800-1000 mg were not significant(P gt;0.05).
ConclusionPNGS relieve the injury of the rat liver during cold preservation. The mechanism might through inhibition calcium overload, improve the energy metabolism, play a role against free radical injury realize.
Citation：
JIANG Nan,LI Li,GUO Yongzhang.. Experimental Study on Cold Preservation of Rat Liver with Panax Notoginseng Saponins. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2005, 12(2): 153-157. doi:
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- 1. 王作仁，石景森，田和平，等. 分离大白鼠肝细胞的低温储存研究 [Ｊ]. 中华实验外科杂志，1995； 12（5） 269.
- 2. 徐东波，杜志，Potts DJ,等. 大鼠离体肝再灌注模型（IPRL）的建立及其在肝移植器官保存液实验中的意义 [Ｊ].生物医学工程与临床， 2001； 5（1）1.
- 3. 马丽英，张乃忠，刑虹，等. 快速等浓度洗脱高效液相色谱法测定心肌组织中的能量物质 [Ｊ]. 中国病理生理杂志， 1992； 8（2）140.
- 4. Kass GE, Llopis J, Chow SC, et al. Receptoroperated calcium influx in rat hepatocytes. Identification and characterization using manganese [Ｊ]. J Biol Chem, 1990; 265(29)17486.
- 5. 彭宝刚，吕明德，肖定璋，等. 三七总皂甙对大鼠肝细胞钙内流的阻断作用 [Ｊ]. 中国药理学通报， 1997； 13（1）70.
- 6. 吕明德，黄嘉凌，肖定璋，等. 三七总皂甙抑制肝细胞钙超载的机制 [Ｊ]. 中国药理学通报， 1999； 15（2）150.
- 7. 陈锋，王天成，王焱林，等. 三七总皂甙及单体Rb1对大鼠心肌缺血再灌注的保护作用 [Ｊ]. 医学新知杂志， 2001； 11（1）31.
- 8. 龚国清，张纯，周曙，等. 人参皂甙等清除超氧阴离子自由基的研究 [Ｊ]. 中国药科大学学报， 1991； 22（1）41.
- 9. Orii T, Ohkohchi N, Satomi S, et al. Assessment of liver graft function after cold preservation using 31P and 23Na magnetic resonance spectroscopy [Ｊ]. Transplantation, 1992; 53(4)730.
