Effects of Preservative Temperature on Pancreatic Function Following Pancreaticoduodenal Transplantation in Rats_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

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YUAN Chunhui , LIU Yongfeng , CHENG Ying , ZHAO Ning , LIANG Jiang , HE Sanguang.

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Pancreatic transplantation Organ preservation Preservative temperature

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【Abstract】Objective To investigate the effects of preservative temperature on pancreatic function and determine the optimal preservative temperature for pancreatic transplantation.
MethodsCold pancreatic preservation was performed and a homologous male Wistar rat model of heterotopic total pancreaticoduodenal transplantation was established. The pancreas was preserved for 6 h in UW solution at specific temperatures(0 ℃, 4 ℃, 8 ℃ and 12 ℃). After preservation, pancreatic tissue was taken for histologic examination in every group. ATP and total adenine nucleotides (TAN) were determined by using high performance liquid chromatography (HPLC). Blood glucose(BG), serum amylase and lipase were measured 24 h after transplantation. And the activity of myeloperoxidase (MPO) in the pancreatic grafts was also measured at the same time. Besides, histological observation was performed.
Results Microscopic studies showed that the histomorphological changes of pancreas in 4 ℃ group were less obvious than those in other groups. Tissual concentrations of ATP and TAN decreased gradually in 4 ℃ group, 0 ℃ group, 8 ℃ group, and 12 ℃ group after 6 h of preservation(PH＜0.05). The levels of BG, serum lipase and MPO increased gradually in 4 ℃ group, 0 ℃ group, 8 ℃ group, and 12 ℃ group(PH＜0.05). The activity of MPO in 4 ℃ group （1.19±0.16 U/g ）was significantly lower than that of the control group（0.26±0.09 U/g，PH＜0.05).
Conclusion The temperature of 4 ℃ is most appropriate for hypothermic pancreatic preservation and can considerably alleviate cold ischemic injury of rat pancreas.

Citation： YUAN Chunhui,LIU Yongfeng,CHENG Ying,ZHAO Ning,LIANG Jiang,HE Sanguang.. Effects of Preservative Temperature on Pancreatic Function Following Pancreaticoduodenal Transplantation in Rats. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2005, 12(4): 365-368. doi: Copy

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1. Troisi R, Meester D, Regaert B, et al. Tolerance of the porcine pancreas to warm and cold ischemia: comparison between University of Wisconsin and histidinetryptophanketoglutarate solution [Ｊ]. Transplant Proc,2002; 34(3)∶ 820.
2. Lee S, Tung KSK, Koopmans H, et al. Pancreaticoduodenal transplantation in the rat [Ｊ]. Transplantation, 1971; 13(6)∶ 421.
3. Kwon AH, Inui H, Yoshida K, et al. Pancreatic islet preservation at temperatures just above the freezing point [Ｊ]. Transplant Proc,2003; 35(1)∶ 480.
4. Ludwig S, Armann B, Escher E, et al. Pathomorphologic and microcirculatory changes and endothelin1 expression in UWand Celsiorpreserved pancreata in experimental pancreas transplantation [Ｊ]. Transplant Proc,2002; 34(6)∶ 2364.
5. Mahmoud MS, Wang P, Chaudry IH. Salutary effects of ATPMgCl2 on altered hepatocyte signal transduction after hemorrhagic shock [Ｊ]. Am J Physiol，1997; 272(6)∶ 1347.
6. Lakey JR, Kneteman NM, Rajotte RV, et al. Effect of core pancreas temperature during cadaveric procurement on human islet isolation and functional viability [Ｊ]. Transplantation,2002; 73(7)∶ 1106.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effects of Preservative Temperature on Pancreatic Function Following Pancreaticoduodenal Transplantation in Rats

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