Effect of IL-10 and IL-18 on Acute Lung Injury of Severe Acute Pancreatitis_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

WANG Xiankun ¹ , ZHAN Leheng ¹ , WANG Shiwen ¹ , ZHANG Youcheng ² , WANG Yuliang ³ , KANG Yingxin. ¹

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Keywords：

Pancreatitis Lung injury Interleukin-10 Interleukin-18 Gene expression

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【Abstract】Objective To investigate the role of interleukin-10（IL-10） and interleukin-18 （IL-18） in the pathogenesis of acute lung injury in experimental severe acute pancreatitis.
Methods Forty-eight SD rats were divided into control group and SAP group by the random data table. The model of experimental severe acute pancreatitis was established by injection of 3.5% sodium taurocholate into the bili-pancreatic duct. Lung wet weight index, ascities and level of serum amylase, IL-10 and IL-18 were quantitatively measured in different time. Intrapulmonary expressions of IL-10 mRNA and IL-18 mRNA were detected by semiquantitative RTPCR. The histopathology of pancreas and lung were observed under the light microscope.
Results Lung wet weight index, ascities, level of serum amylase, IL-10 and IL-18, intrapulmonary expressions of IL-10 mRNA and IL-18 mRNA were significantly increased in SAP group (P＜0.01). The level of serum IL-18 and intrapulmonary expression of IL-18mRNA are positively correlated with lung wet weight index （r=0.68,P＜0.01; r=0.72,P＜0.01） and lung injury score （r=0.74,P＜0.01; r=0.79,P＜0.01) respectively, whereas the level of serum IL-10 and intrapulmonary expression of IL-10 mRNA are negatively correlated with lung wet weight index（r=-0.62,P＜0.01; r=-0.69,P＜0.01） and lung injury score（r=-0.66,P＜0.01; r=-0.60,P＜0.01）.
Conclusion IL-18 may play a key role in the pathogenesis of acute lung injury in experimental severe acute pancreatitis, and IL-10 exerts the protection role in this process.

Citation： WANG Xiankun,ZHAN Leheng,WANG Shiwen,ZHANG Youcheng,WANG Yuliang,KANG Yingxin.. Effect of IL-10 and IL-18 on Acute Lung Injury of Severe Acute Pancreatitis. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2005, 12(5): 443-446. doi: Copy

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1. Shields CJ, Winter DC, Redmond HP. Lung injury in acute pancreatitis: mechanisms, prevention, and therapy [Ｊ]. Curr Opin Crit Care， 2002; 8(2)∶158.
2. Kusske AM, Rongione AJ, Ashley SW, et al. Interleukin10 prevents death in lethal necrotizing pancreatitis in mice [Ｊ]. Surgery, 1996; 120(2)∶284.
3. Denham W, Yang J, Fink G, et al. Gene targeting demonstrates additive detrimental effects of interleukin 1 and tumor necrosis factor during pancreatitis [Ｊ]. Gastroenterology, 1997; 113(5)∶1741.
4. Hofbauer B, Saluja AK, Bhatia M, et al. Effect of recombinant plateletactivating factor acetylhydrolase on two models of experimental acute pancreatitis [Ｊ]. Gastroenterology, 1998; 115(5)∶1238.
5. Norman J. The role of cytokines in the pathogenesis of acute pancreatitis [Ｊ]. Am J Surg, 1998； 175(1)∶76.
6. Leung BP, Culshaw S, Gracie JA, et al. A role for IL18 in neutrophil activation [Ｊ]. J Immunol, 2001; 167(5)∶2879.
7. Dinarello CA. IL18: A TH1inducing, proinflammatory cytokine and new member of the IL1 family [Ｊ]. J Allergy Clin Immunol, 1999; 103(1 Pt 1)∶11.
8. Jordan JA, Guo RF, Yun EC, et al. Role of IL18 in acute lung inflammation [Ｊ]. J Immunol, 2001; 167(12)∶7060.
9. Imrie CW, McKay CJ. The scientific basis of medical therapy of acute pancreatitis. Could it work, and is there a role for lexipafant? [Ｊ]. Gastroenterol Clin North Am, 1999; 28(3)∶591.
10. WereszczynskaSiemiatkowska U, Dabrowski A, Siemiatkowski A, et al. Serum profiles of Eselectin, interleukin10, and interleukin6 and oxidative stress parameters in patients with acute pancreatitis and nonpancreatic acute abdominal pain [Ｊ]. Pancreas, 2003; 26(2)∶144.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effect of IL-10 and IL-18 on Acute Lung Injury of Severe Acute Pancreatitis

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