• Department of General Surgery, The First Affiliated Hospital, Xinxiang Medical College, Weihui 453100;
Arbitrarily primer polymerase chain reaction Gastric adenocarcinoma Genome variationCloningSouthern blot hybridization
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Objective  To identify and isolate the variant gene associated with gastric adenocarcinoma and clone the fragment of variant gene.
Methods  By arbitrarily primer polymerase chain reaction (AP-PCR), DNA samples from 5 matched gastric adenocarcinoma and non-tumor gastric tissues were analysed.
Results  The produced AP-PCR profiles were different in each matched gastric adenocarcinoma and non-tumor gastric tissue. One differentiated amplified DNA fragments PW2.2 from a matched gastric adenocarcinoma were cloned. The result of Southern blot hybridization with PW2.2 as a probe showing that this fragment was also found in some other gastric adenocarcinoma samples.
Conclusion  AP-PCR fingerprinting assay can be used to identify and clone the variant genes associated with gastric adenocarcinoma.

Citation： YAN Zhengqiang,LU Fengmin,WANG Yanxin,et al.. A STUDY ON THE GENOMIC VARIANT IN MATCHED ADENOCINOMA AND NON-TUMOR GASTRIC TISSUE BY ARBITRARILY PRIMER POLYMERASE CHAIN REACTION. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2000, 7(3): 144-146. doi: Copy