Objective To identify and isolate the variant gene associated with gastric adenocarcinoma and clone the fragment of variant gene.
Methods By arbitrarily primer polymerase chain reaction (AP-PCR), DNA samples from 5 matched gastric adenocarcinoma and non-tumor gastric tissues were analysed.
Results The produced AP-PCR profiles were different in each matched gastric adenocarcinoma and non-tumor gastric tissue. One differentiated amplified DNA fragments PW2.2 from a matched gastric adenocarcinoma were cloned. The result of Southern blot hybridization with PW2.2 as a probe showing that this fragment was also found in some other gastric adenocarcinoma samples.
Conclusion AP-PCR fingerprinting assay can be used to identify and clone the variant genes associated with gastric adenocarcinoma.
Citation：
YAN Zhengqiang,LU Fengmin,WANG Yanxin,et al.. A STUDY ON THE GENOMIC VARIANT IN MATCHED ADENOCINOMA AND NON-TUMOR GASTRIC TISSUE BY ARBITRARILY PRIMER POLYMERASE CHAIN REACTION. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2000, 7(3): 144-146. doi:
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- 1. 卢圣栋主编. 现代分子生物学技术与方法 〔Ｍ〕. 北京： 中国科学技术出版社， 1993∶33～38.
- 2. 刘霜，芮静安，鲁凤民等. 配对肝癌组织与非癌肝组织基因差异的随机多态性DNA分析 〔Ｊ〕. 北京医科大学学报， 1996； 28(6)∶408.
- 3. 刘霜， 鲁凤民. 一种简便的质粒DNA快速提取方法 〔Ｊ〕. 北京医科大学学报， 1996； 28(3)∶182.
- 4. 鲁凤民， 张贵寅， 杜杨拄等. 一个进行性肌营养不良家系的携带者检出及基因诊断 〔Ｊ〕. 中华神经精神科杂志， 1990； 23(4)∶231.
- 5. Peinado MA, Malkhosyan S, Velazquez A, et al. Isolation and characterization of allelic losses and gains in colorectal tumors by arbitrarily primed polymerase chain reaction 〔Ｊ〕. Pro Natl Acad Sci USA, 1992; 89(17)∶10065.
- 6. Yang XL, Nakao Y, Pater MM, et al. Identification of two novel cellular genes with multistage carcinogenesis of human endocervical cells by mRNA differential display 〔Ｊ〕. Carcinogenesis, 1996; 17(3)∶563.
- 7. 孟祥文， 李璞. APPCR技术及应用 〔Ｊ〕. 国外医学遗传学分册， 1995； 18(3)∶213.
- 8. 顾建人. 抑癌基因及人原发性胃腺癌中抑癌基因研究进展 〔Ｊ〕. 肿瘤， 1995； 15(3)∶105.
