THE CLINICAL SIGNIFICANCE OF TELOMERASE ACTIVITY IN LIVER CANCER AND PRE-CANCEROUS LESION_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

WANG Wei ¹ , YANG Shanmin ² , LI Xinfeng ¹ , et al. ³

Department of General Surgery, The Second Affiliated Hospital, Fujian University of Medical Sciences, Quanzhou 362000;

Corresponding author：

Keywords：

Liver cancer Precancer lesion Telomerase

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Objective To investigate the variety of telomerase activity in the course of liver cancer development, and the possibility of using telomerase as a marker of HCC.
Methods Human liver specimens, comprising 22 HCC and adjacent peritumoral tissues, 12 liver cirrhosistissues, 6 nodulat regenerative hyperplasia (NRH) tissues and 10 normal liver tissues, were examined for telomerase activity by TRAP assay based on PCR.
Results Twenty of 22 HCC and 14 of 22 adjacent tissue specimens were positive for telomerase activity with a positive rate of 90.9% and 63.6% respectively. Ten of 12 liver cirrhosis tissues were positive with a positive rate of 83.3%. 5 of 6 NRH were positive with a positive rate of 83.3%. Telomerase activity was negative in 10 normal liver tissues.
Conclusion Telomerase may occur in the progress of hepatocarcinogenesis. Telomerase can be used as a tumor marker of HCC.

Citation： WANG Wei,YANG Shanmin,LI Xinfeng,et al.. THE CLINICAL SIGNIFICANCE OF TELOMERASE ACTIVITY IN LIVER CANCER AND PRE-CANCEROUS LESION. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2000, 7(4): 246-248. doi: Copy

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2.	Kim NW, Piatyszek MA, Prowse KR, et al. Specific association of human telomerase activity with immoratal cells and cancer 〔Ｊ〕. Science, 1994; 226(5193)∶2011.
3.	Chadenean C, Hay K, Hirte HW, et al. Telomerase activity associated with acquistion of malignancy in human colorectal cancer 〔Ｊ〕. Cancer Res, 1995; 55(12)∶2573.
4.	Counter CM, Cuptal J, Harley CB, et al. Telomerase activity in normal leukcoytes and in hematologic malignancies 〔Ｊ〕. Blood, 1995; 85(9)∶2315.
5.	Tsujiuchi T, Tustsumi M, Kido A, et al. Increase telomerase activity in hyperplastic nodules and hepatocellular carcinomas induced by a cholinedeficient Lamino aciddefined diet in rats 〔Ｊ〕. Japan J Cancer Research, 1996; 87(11)∶1111.
6.	Ohnishi M, Univ G, Med S, et al. Inhibitory effects of dietary protocatechuic acid and costunolide on 7, 12dimethybenz [a] anthraceneinduced hamster cheek pouch carcinogenesis 〔Ｊ〕. Japan J Cancer Research, 1997; 88(2)∶111.
7.	Takara H, Nakanish T, Kitamoto M, et al. Telomerase activity in human liver tissues; comparison between chronic liver disease and hepatocellular carcinoma 〔Ｊ〕. Cancer Ris, 1995; 55(13)∶2734.
8.	Kojima H, Yokosuka F, Imazeki F, et al. Telomerase activity and telomere length in hepatocellular carcinoma and chronic liver disease 〔Ｊ〕. Gastroenterology, 1997; 112(2)∶493.
9.	Nakashiv K, Kitamoto M, Takara H, et al. Significance of telomerase activity of small differentiated hepatocellular carcinoma 〔Ｊ〕. Int J Cancer, 1997; 74(2)∶141.
10.	Miura N, Horikawa I, Nashimoto H, et al. Progressive telomerase shortening and reactivation during hepatocellular carcinogenesis 〔Ｊ〕. Cancer Genet Cytogent, 1997; 93(1)∶56.
11.	Bednarek A, Budunova I, Slaga TJ, et al. Increased telomerase activity in mouse skin premalignant progression 〔Ｊ〕. Cancer Res, 1995; 55(2)∶4566.

1. 关晓飞，孙志贤主编. 端粒酶活性检测及其在恶性肿瘤诊断中的应用〔M〕. 北京：军事医学科学出版社， 1992∶49～56.
2. Kim NW, Piatyszek MA, Prowse KR, et al. Specific association of human telomerase activity with immoratal cells and cancer 〔Ｊ〕. Science, 1994; 226(5193)∶2011.
3. Chadenean C, Hay K, Hirte HW, et al. Telomerase activity associated with acquistion of malignancy in human colorectal cancer 〔Ｊ〕. Cancer Res, 1995; 55(12)∶2573.
4. Counter CM, Cuptal J, Harley CB, et al. Telomerase activity in normal leukcoytes and in hematologic malignancies 〔Ｊ〕. Blood, 1995; 85(9)∶2315.
5. Tsujiuchi T, Tustsumi M, Kido A, et al. Increase telomerase activity in hyperplastic nodules and hepatocellular carcinomas induced by a cholinedeficient Lamino aciddefined diet in rats 〔Ｊ〕. Japan J Cancer Research, 1996; 87(11)∶1111.
6. Ohnishi M, Univ G, Med S, et al. Inhibitory effects of dietary protocatechuic acid and costunolide on 7, 12dimethybenz [a] anthraceneinduced hamster cheek pouch carcinogenesis 〔Ｊ〕. Japan J Cancer Research, 1997; 88(2)∶111.
7. Takara H, Nakanish T, Kitamoto M, et al. Telomerase activity in human liver tissues; comparison between chronic liver disease and hepatocellular carcinoma 〔Ｊ〕. Cancer Ris, 1995; 55(13)∶2734.
8. Kojima H, Yokosuka F, Imazeki F, et al. Telomerase activity and telomere length in hepatocellular carcinoma and chronic liver disease 〔Ｊ〕. Gastroenterology, 1997; 112(2)∶493.
9. Nakashiv K, Kitamoto M, Takara H, et al. Significance of telomerase activity of small differentiated hepatocellular carcinoma 〔Ｊ〕. Int J Cancer, 1997; 74(2)∶141.
10. Miura N, Horikawa I, Nashimoto H, et al. Progressive telomerase shortening and reactivation during hepatocellular carcinogenesis 〔Ｊ〕. Cancer Genet Cytogent, 1997; 93(1)∶56.
11. Bednarek A, Budunova I, Slaga TJ, et al. Increased telomerase activity in mouse skin premalignant progression 〔Ｊ〕. Cancer Res, 1995; 55(2)∶4566.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

THE CLINICAL SIGNIFICANCE OF TELOMERASE ACTIVITY IN LIVER CANCER AND PRE-CANCEROUS LESION

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