STUDIES OF THE RELATIONSHIP BETWEEN ISLETS OF LANGERHANS TRANSPLANTATION AND MICROCIRCULATION_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

CHEN Chuangqi , LAN Ping

Department of Gastrointestinal and Pancreatic Surgery, The First Affiliated Hospital, Sun YatSen University of Medical Sciences, Guangzhou 510080;

Corresponding author：

Keywords：

Islets of Langerhans transplantation Microcirculation Angiogenesis

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Objective To study the advances in microcirculation after islets of Langerhans transplantation (ILT).
Methods The literature in the recent years on the study of the relationship between ILT and microcirculation was reviewed.
Results The process of angiogenesis and revascularization of the islet grafts was in progress within 1 week after transplantation, and was completed within 10-14 days after transplantation, exhibiting a microangioarchitecture similar to pancreatic islets in situ. The sequence of vascular intraislet cellular perfusion was from β cells outward to α-and δ-cell cortex, with the majority of α cells perfused before the majority of δ cells. Freely transplanted islet grafts were revascularized from the hostderived microvascular bed. The interstitial pressure in the islet transplants was markedly lower than the capillary pressure. There were clearly differences in microcirculation between syngeneic and xenogeneic islet grafts. The phenomena of microcirculation failure were observed in xenografts. The influential factors of microcirculation after ILT were ①culture temperature of isolated islets, ②cultured time and cryopreserved method of islets, ③blood glucose, ④immunosuppressive agents, ⑤angiogenesis factors.
Conclusion Microvascularization of freely islet grafts is one of the essential requirements for successful engraftment, guaranteeing sufficient nutritional blood supply to the tissue and establishing blood drainage for adequate liberation of the endocrine hormones. Through the studies of the microcirculation after ILT, it is helpful to recognize the mechanism of the survival of islet grafts.

Citation： CHEN Chuangqi,LAN Ping,ZHAN Wenhua.. STUDIES OF THE RELATIONSHIP BETWEEN ISLETS OF LANGERHANS TRANSPLANTATION AND MICROCIRCULATION. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2000, 7(6): 411-414. doi: Copy

1.	Menger MD, Jaeger S, Walter P, et al. Angiogenesis and hemodynamics of microvasculature of transplanted islets of Langerhans 〔Ｊ〕. Diabetes, 1989; 38(Suppl 1)∶199.
2.	Vajkoczy P, Olofsson AM, Lehr HA, et al. Histogenesis and ultrastructure of pancreatic islet graft microvasculature. Evidence for graft revascularization by endothelial cells of host origin 〔Ｊ〕. Am J Pathol, 1995; 146(6)∶1397.
3.	Jansson L, Sandler S. Influence of hyperglycemia on blood perfusion of autotransplanted pancreatic islets in diabetic rats 〔Ｊ〕. Diabetes, 1989; 38(Suppl 1)∶196.
4.	Vajkoczy P, Menger MD, Simpson E, et al. Angiogenesis and vascularization of murine pancreatic islet isografts 〔Ｊ〕. Transplantation, 1995; 60(2)∶123.
5.	Merchant FA, Aggarwal SJ, Diller KR, et al. Invivo analysis of angiogenesis and revascularization of transplanted pancreatic islets using confocal microscopy 〔Ｊ〕. J Microsc, 1994; 176(pt 3)∶262.
6.	Borud LJ, Shaw WW, Brunicardi FC, et al. Prefabrication of a neoendocrine organ: a rat model 〔Ｊ〕. J Surg Res, 1996; 61(1)∶221.
7.	Carlsson PO, Jansson L, Andersson A, et al. Capillary blood pressure in syngeneic rat islets transplanted under the renal capsule is similar to that of the implantation organ 〔Ｊ〕. Diabetes, 1998; 47(10)∶1586.
8.	Yamauchi J, Wolf B, Vollmar B, et al. In vivo analysis of the origin of capillary sprout formation in angiogenesis of freely transplanted islets of Langerhans 〔Ｊ〕. Adv Exp Med Biol, 1998; 454(3)∶355.
9.	Tatarkiewicz K, HollisterLock J, Quickel RR, et al. Reversal of hyperglycemia in mice after subcutaneous transplantation of maroencapsulated islets 〔Ｊ〕. Transplantation, 1999; 67(5)∶665.
10.	Beger C, Cirulli V, Vajkoczy P, et al. Vascularization of purified pancreatic isletlike cell aggregates (pseudoislets) after syngeneic transplantation 〔Ｊ〕. Diabetes, 1998; 47(4)∶559.
11.	Merchant FA, Diller KR, Aggarwal SJ, et al. Angiogenesis in cultured and cryopreserved pancreatic islet grafts 〔Ｊ〕. Transplantation, 1997; 63(11)∶1652.
12.	Menger MD, Beger C, Vajkoczy P. Restitution of intraislet portal system in pancreatic islet isografts 〔Ｊ〕. Transplant Proc, 1994; 26(2)∶688.
13.	Menger MD, Vajkoczy P, Beger C, et al. Orientation of microvascular blood flow in panreatic islet isografts 〔Ｊ〕. J Clin Invest, 1994; 93(5)∶2280.
14.	Risau W. Mechanisms of angiogenesis 〔Ｊ〕. Nature, 1997; 386∶671.
15.	Menger MD, Jger S, Wolf B, et al. The influence of cyclosporine on the microvasculature of xenogeneic pancreatic islet grafts 〔Ｊ〕. Transplant Proc, 1991; 23(4)∶2217.
16.	Menger MD, Jger S, Walter P, et al. Influence of culture temperature(24℃/37℃) on revascularization of transplanted islets of Langerhans in the Syrian hamster 〔Ｊ〕. Transplant Proc, 1990; 22(2)∶823.
17.	Mendola JF, FernndezAlvarez J, Saenz A, et al. Effect of hyperglycemia and of oneweek culture of islets on the revascularization of pancreatic islet isografts 〔Ｊ〕. Transplant Proc, 1994; 26(2)∶689.
18.	Menger MD, Jger S, Walter P, et al. The influence of (±)15deoxyspergualin on revascularization of xenogeneic transplanted islets of Langerhans 〔Ｊ〕. Transplant Proc, 1990; 22(4)∶1631.
19.	Vajkoczy P, Vollmar B, Wolf B, et al. Effects of cyclosporine A on the process of vascularization of freely transplanted islets of Langerhans 〔Ｊ〕. J Mol Med, 1999; 77(1)∶111.
20.	Menger MD, Wolf B, Jger S, et al. The influence of prednisolone on revascularization of pancreatic islet grafts 〔Ｊ〕. Transplant Proc, 1990; 22(4)∶2042.
21.	Menger MD, Vajkoczy P, Leiderer R, et al. Influence of experimental hyperglycemia on microvascular blood perfusion of pancreatic islet isografts 〔Ｊ〕. J Clin Invest, 1992; 90(4)∶1361.
22.	Menger MD, Jger S, Walter P, et al. Influence of hyperglycemia on the process of angiogenesis and revascularization of freely transplanted islets of Langerhans 〔Ｊ〕. Transplant Proc, 1990; 22(2)∶821.
23.	Stagner JI, Mokshagundam S, Samols E. Hormone secretion from transplanted islets is dependent upon changes in islet revascularization and architecture 〔Ｊ〕. Transplant Proc, 1995; 27(6)∶3251.
24.	Stagner JI, Samols E. The vascular order of islet cellular perfusion in the human pancreas 〔Ｊ〕. Diabetes, 1992; 41(1)∶93.
25.	Stagner JI, Samols E. Altered microcirculation and secretion in tranplanted islets 〔Ｊ〕. Transplant Proc, 1994; 26(3)∶1100.
26.	Hayek A, Beattie GM, Lopez AD, et al. The use of digital image processing to quantitate angiogenesis induced by basic fibroblast growth factor and transplanted pancreatic islets 〔Ｊ〕. Microvasc Res, 1991; 41(2)∶203.

1. Menger MD, Jaeger S, Walter P, et al. Angiogenesis and hemodynamics of microvasculature of transplanted islets of Langerhans 〔Ｊ〕. Diabetes, 1989; 38(Suppl 1)∶199.
2. Vajkoczy P, Olofsson AM, Lehr HA, et al. Histogenesis and ultrastructure of pancreatic islet graft microvasculature. Evidence for graft revascularization by endothelial cells of host origin 〔Ｊ〕. Am J Pathol, 1995; 146(6)∶1397.
3. Jansson L, Sandler S. Influence of hyperglycemia on blood perfusion of autotransplanted pancreatic islets in diabetic rats 〔Ｊ〕. Diabetes, 1989; 38(Suppl 1)∶196.
4. Vajkoczy P, Menger MD, Simpson E, et al. Angiogenesis and vascularization of murine pancreatic islet isografts 〔Ｊ〕. Transplantation, 1995; 60(2)∶123.
5. Merchant FA, Aggarwal SJ, Diller KR, et al. Invivo analysis of angiogenesis and revascularization of transplanted pancreatic islets using confocal microscopy 〔Ｊ〕. J Microsc, 1994; 176(pt 3)∶262.
6. Borud LJ, Shaw WW, Brunicardi FC, et al. Prefabrication of a neoendocrine organ: a rat model 〔Ｊ〕. J Surg Res, 1996; 61(1)∶221.
7. Carlsson PO, Jansson L, Andersson A, et al. Capillary blood pressure in syngeneic rat islets transplanted under the renal capsule is similar to that of the implantation organ 〔Ｊ〕. Diabetes, 1998; 47(10)∶1586.
8. Yamauchi J, Wolf B, Vollmar B, et al. In vivo analysis of the origin of capillary sprout formation in angiogenesis of freely transplanted islets of Langerhans 〔Ｊ〕. Adv Exp Med Biol, 1998; 454(3)∶355.
9. Tatarkiewicz K, HollisterLock J, Quickel RR, et al. Reversal of hyperglycemia in mice after subcutaneous transplantation of maroencapsulated islets 〔Ｊ〕. Transplantation, 1999; 67(5)∶665.
10. Beger C, Cirulli V, Vajkoczy P, et al. Vascularization of purified pancreatic isletlike cell aggregates (pseudoislets) after syngeneic transplantation 〔Ｊ〕. Diabetes, 1998; 47(4)∶559.
11. Merchant FA, Diller KR, Aggarwal SJ, et al. Angiogenesis in cultured and cryopreserved pancreatic islet grafts 〔Ｊ〕. Transplantation, 1997; 63(11)∶1652.
12. Menger MD, Beger C, Vajkoczy P. Restitution of intraislet portal system in pancreatic islet isografts 〔Ｊ〕. Transplant Proc, 1994; 26(2)∶688.
13. Menger MD, Vajkoczy P, Beger C, et al. Orientation of microvascular blood flow in panreatic islet isografts 〔Ｊ〕. J Clin Invest, 1994; 93(5)∶2280.
14. Risau W. Mechanisms of angiogenesis 〔Ｊ〕. Nature, 1997; 386∶671.
15. Menger MD, Jger S, Wolf B, et al. The influence of cyclosporine on the microvasculature of xenogeneic pancreatic islet grafts 〔Ｊ〕. Transplant Proc, 1991; 23(4)∶2217.
16. Menger MD, Jger S, Walter P, et al. Influence of culture temperature(24℃/37℃) on revascularization of transplanted islets of Langerhans in the Syrian hamster 〔Ｊ〕. Transplant Proc, 1990; 22(2)∶823.
17. Mendola JF, FernndezAlvarez J, Saenz A, et al. Effect of hyperglycemia and of oneweek culture of islets on the revascularization of pancreatic islet isografts 〔Ｊ〕. Transplant Proc, 1994; 26(2)∶689.
18. Menger MD, Jger S, Walter P, et al. The influence of (±)15deoxyspergualin on revascularization of xenogeneic transplanted islets of Langerhans 〔Ｊ〕. Transplant Proc, 1990; 22(4)∶1631.
19. Vajkoczy P, Vollmar B, Wolf B, et al. Effects of cyclosporine A on the process of vascularization of freely transplanted islets of Langerhans 〔Ｊ〕. J Mol Med, 1999; 77(1)∶111.
20. Menger MD, Wolf B, Jger S, et al. The influence of prednisolone on revascularization of pancreatic islet grafts 〔Ｊ〕. Transplant Proc, 1990; 22(4)∶2042.
21. Menger MD, Vajkoczy P, Leiderer R, et al. Influence of experimental hyperglycemia on microvascular blood perfusion of pancreatic islet isografts 〔Ｊ〕. J Clin Invest, 1992; 90(4)∶1361.
22. Menger MD, Jger S, Walter P, et al. Influence of hyperglycemia on the process of angiogenesis and revascularization of freely transplanted islets of Langerhans 〔Ｊ〕. Transplant Proc, 1990; 22(2)∶821.
23. Stagner JI, Mokshagundam S, Samols E. Hormone secretion from transplanted islets is dependent upon changes in islet revascularization and architecture 〔Ｊ〕. Transplant Proc, 1995; 27(6)∶3251.
24. Stagner JI, Samols E. The vascular order of islet cellular perfusion in the human pancreas 〔Ｊ〕. Diabetes, 1992; 41(1)∶93.
25. Stagner JI, Samols E. Altered microcirculation and secretion in tranplanted islets 〔Ｊ〕. Transplant Proc, 1994; 26(3)∶1100.
26. Hayek A, Beattie GM, Lopez AD, et al. The use of digital image processing to quantitate angiogenesis induced by basic fibroblast growth factor and transplanted pancreatic islets 〔Ｊ〕. Microvasc Res, 1991; 41(2)∶203.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

STUDIES OF THE RELATIONSHIP BETWEEN ISLETS OF LANGERHANS TRANSPLANTATION AND MICROCIRCULATION

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