Effects of Exogenous Hydrogen Sulfide on Acute Necrotizing Pancreatitis_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

ZHANG Yong ¹ , LI Bin ¹ , LU Yan ² , CHENG Dongsheng ² , CHAI Chen ¹ , XIE Baoping ¹ ,  CAO Nong ¹

1. The First Department of General Surgery， The First Hospital of Lanzhou University， Lanzhou 730000， Gansu Province， China;;
2. Department of Clinical Laboratory， The First Hospital of Chinese People’s Liberation Army， Lanzhou 730030， Gansu Province， China;

Corresponding author：

CAO Nong, Email: caonong1955@yahoo.cn

Keywords：

Acute necrotizing pancreatitis; Hydrogen sulfide; Liver; Kidney; Pancreas

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Abstract Full text Figures/Tables Video References Cited by

Objective 　To explore the influences of hydrogen sulfide （H2S） on acute necrotizing pancreatitis （ANP）.
Methods 　Forty-three SD male rats were grouped by random number table， and divided into five groups：the sham group （n=4）， ANP group 〔n=21， which was divided into 3 subgroups：3， 6， and 12 hours group （n=7）〕， NaCl＋ANP group （n=4）， NaHS＋ANP group （n=7）， and PAG＋ANP group （n=7）. Models of ANP were formed by
retrograde cholangiopancreatography injection of 5% sodium taurocholate. The NaCl＋ANP group， NaHS＋ANP group， and PAG＋ANP group rats were given pretreatment of saline， NaHS， or PAG at 1 hour before modeling
respectively. The levels of serum amylase （AMY）， aspartate aminotransferase （AST）， alanine aminotransferase （ALT）， blood urea nitrogen （BUN）， and creatinine （Cr） were detected， and the pathological histological changes of pancreatic tissues were observed.
Results 　The levels of serum AMY， AST， ALT， BUN， and Cr were increased in ANP group. The levels of serum AMY， AST， ALT， BUN， and Cr in the NaHS＋ANP group were higher than those of NaCl＋ANP group （P＜0.05）， and the pathological damage of the pancreatic tissues was more serious in the NaHS＋ANP group. The levels of serum AMY， AST， ALT， BUN， and Cr in the PAG＋ANP group were lower than those of NaCl＋ANP
group （P＜0.05）， and the pathological damage of pancreatic tissues in the PAG＋ANP group was not so serious as in the NaCl＋ANP group.
Conclusions 　The impairment of liver， kidney， and pancreas function in ANP rats may be related to elevated H2S concentration. Prophylactic administration the PAG of H2S antagonist can improve the function of the liver， kidney， and pancreas， and have the effects of organ protection.

Citation： ZHANG Yong,LI Bin,LU Yan,CHENG Dongsheng,CHAI Chen,XIE Baoping,CAO Nong. Effects of Exogenous Hydrogen Sulfide on Acute Necrotizing Pancreatitis. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2013, 20(4): 385-388. doi: Copy

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14.	Zhang H， Zhi L， Moore PK， et al. Role of hydrogen sulfide in cecal ligation and puncture-induced sepsis in the mouse[J]. Am J Physiol Lung Cell Mol Physiol， 2006， 290(6)：L1193- L1201.
15.	Zhang J， Sio SW， Moochhala S， et al. Role of hydrogen sulfide in severe burn injury-induced inflammation in mice[J]. Mol Med， 2010， 16(9-10)：417-424.
16.	Li L， Bhatia M， Zhu YZ， et al. Hydrogen sulfide is a novel mediator of lipopolysaccharide-induced inflammation in the mouse[J]. FASEB J， 2005， 19(9)：1196-1198.
17.	Zhang Q， Ni Q， Cai D， et al. Mechanisms of multiple organ damages in acute necrotizing pancreatitis[J]. Chin Med J (Engl)， 2001， 114(7)：738-742.
18.	Yang R， Shaufl AL， Killeen ME， et al. Ethyl pyruvate amelioratesliver injury secondary to severe acute pancreatitis[J]. J Surg Res， 2009， 153(2)：302-309.
19.	李峥艳，张凌，金涛，等. CT评分与重症急性胰腺炎合并急性肾损伤的相关性分析[J]. 四川大学学报：医学版， 2010， 42(5)：695-698， 703.
20.	Bhatia M， Wong FL， Fu D， et al. Role of hydrogen sulfide in acute pancreatitis and associated lung injury[J]. FASEB J， 2005， 19(6)：623-625.
21.	Tamizhselvi R， Moore PK， Bhatia M. Hydrogen sulfide acts as a mediator of inflammation in acute pancreatitis：in vitro studies usingisolated mouse pancreatic acinar cells[J]. J Cell Mol Med， 2007， 11(2)：315-326.
22.	Tamizhselvi R， Shrivastava P， Koh YH， et al. Preprotachykinin-Agene deletion regulates hydrogen sulfide-induced toll-like receptor.
23.	Shanmugam MK， Bhatia M. The role of pro-inflammatory molec-ules and pharmacological agents in acute pancreatitis and sepsis[J]. Inflamm Allergy Drug Targets， 2010， 9(1)：20-31.
24.	陈尼维，沈伟琳，赵祥运，等. 血浆硫化氢在急性胰腺炎损伤中的作用[J]. 同济大学学报：医学版， 2012， 33(1)：94-95， 104.
25.	黄惊鸿，卢根林，斯伟宏，等. 硫化氢在重症急性胰腺炎患者血浆中的表达及意义[J]. 中国中西医结合外科杂志， 2012， 18(1)：25-27.
26.	饶春燕，付兰英，赵晓晏. 硫化氢对急性坏死性胰腺炎及相关肺损伤作用的初步探讨[J]. 中华胰腺病杂志， 2012， 12(2)：129-131.

1. Hajdu N， Belagyi T， Issekutz A， et al. Intravenous glutamine andearly nasojejunal nutrition in severe acute pancreatitis—a prosp-ective randomized clinical study[J]. Magy Seb， 2012， 65(2)：44-51.
2. Ni J， Hu G， Xiong J， et al. Involvement of interleukin-17A in pancreatic damage in rat experimental acute necrotizing pancreatitis[J]. Inflammation， 2013， 36(1)：53-65.
3. Wang R. Two’s company， three’s a crowd：can H2S be the third endogenous gaseous transmitter?[J]. FASEB J， 2002， 16(13)：1792-1798.
4. signaling pathway in cerulein-treated pancreatic acinar cells[J]. Pancreas， 2011， 40(3)：444-452.
5. Shibuya N， Tanaka M， Yoshida M， et al. 3-Mercaptopyruvate sulfurtransferase produces hydrogen sulfide and bound sulfane sulfur in the brain[J]. Antioxid Redox Signal， 2009， 11(4)：703-714.
6. Gadalla MM， Snyder SH. Hydrogen sulfide as a gasotransmitter[J]. J Neurochem， 2010， 113(1)：14-26.
7. Wang R. Physiological implications of hydrogen sulfide：a whiff exploration that blossomed[J]. Physiol Rev， 2012， 92(2)：791-896.
8. 乔玮，柴琛，杨磊，等. 硫化氢对脓毒症大鼠动脉压力反射的影响[J]. 中国普外基础与临床杂志， 2011， 18(4)：374-379.
9. Liu WQ， Chai C， Li XY， et al. The cardiovascular effects of central hydrogen sulfide are related to K(ATP) channels activation[J]. Physiol Res， 2011， 60(5)：729-738.
10. Collin M， Anuar FB， Murch O， et al. Inhibition of endogenous hydrogen sulfide formation reduces the organ injury caused by endotoxemia[J]. Br J Pharmacol， 2005， 146(4)：498-505.
11. 许永春，李兆申，屠振兴，等. 改良逆行胆胰管注射法制备轻重不同两种大鼠急性胰腺炎模型[J]. 第二军医大学学报， 2004， 25(11)：1251-1252.
12. Bhatia M， Zhi L， Zhang H， et al. Role of substance P in hydrogensulfide-induced pulmonary inflammation in mice[J]. Am J PhysiolLung Cell Mol Physiol， 2006， 291(5)：L896- L904.
13. Bhatia M， Sidhapuriwala J， Moochhala SM， et al. Hydrogen sulphide is a mediator of carrageenan-induced hindpaw oedema in the rat[J]. Br J Pharmacol， 2005， 145(2)：141-144.
14. Zhang H， Zhi L， Moore PK， et al. Role of hydrogen sulfide in cecal ligation and puncture-induced sepsis in the mouse[J]. Am J Physiol Lung Cell Mol Physiol， 2006， 290(6)：L1193- L1201.
15. Zhang J， Sio SW， Moochhala S， et al. Role of hydrogen sulfide in severe burn injury-induced inflammation in mice[J]. Mol Med， 2010， 16(9-10)：417-424.
16. Li L， Bhatia M， Zhu YZ， et al. Hydrogen sulfide is a novel mediator of lipopolysaccharide-induced inflammation in the mouse[J]. FASEB J， 2005， 19(9)：1196-1198.
17. Zhang Q， Ni Q， Cai D， et al. Mechanisms of multiple organ damages in acute necrotizing pancreatitis[J]. Chin Med J (Engl)， 2001， 114(7)：738-742.
18. Yang R， Shaufl AL， Killeen ME， et al. Ethyl pyruvate amelioratesliver injury secondary to severe acute pancreatitis[J]. J Surg Res， 2009， 153(2)：302-309.
19. 李峥艳，张凌，金涛，等. CT评分与重症急性胰腺炎合并急性肾损伤的相关性分析[J]. 四川大学学报：医学版， 2010， 42(5)：695-698， 703.
20. Bhatia M， Wong FL， Fu D， et al. Role of hydrogen sulfide in acute pancreatitis and associated lung injury[J]. FASEB J， 2005， 19(6)：623-625.
21. Tamizhselvi R， Moore PK， Bhatia M. Hydrogen sulfide acts as a mediator of inflammation in acute pancreatitis：in vitro studies usingisolated mouse pancreatic acinar cells[J]. J Cell Mol Med， 2007， 11(2)：315-326.
22. Tamizhselvi R， Shrivastava P， Koh YH， et al. Preprotachykinin-Agene deletion regulates hydrogen sulfide-induced toll-like receptor.
23. Shanmugam MK， Bhatia M. The role of pro-inflammatory molec-ules and pharmacological agents in acute pancreatitis and sepsis[J]. Inflamm Allergy Drug Targets， 2010， 9(1)：20-31.
24. 陈尼维，沈伟琳，赵祥运，等. 血浆硫化氢在急性胰腺炎损伤中的作用[J]. 同济大学学报：医学版， 2012， 33(1)：94-95， 104.
25. 黄惊鸿，卢根林，斯伟宏，等. 硫化氢在重症急性胰腺炎患者血浆中的表达及意义[J]. 中国中西医结合外科杂志， 2012， 18(1)：25-27.
26. 饶春燕，付兰英，赵晓晏. 硫化氢对急性坏死性胰腺炎及相关肺损伤作用的初步探讨[J]. 中华胰腺病杂志， 2012， 12(2)：129-131.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effects of Exogenous Hydrogen Sulfide on Acute Necrotizing Pancreatitis

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